{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:22:17Z","timestamp":1771928537938,"version":"3.50.1"},"reference-count":60,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Ionizing radiation causes severe gastrointestinal injury. B-1a cells, predominantly located in the peritoneal cavity (PerC), play a critical role in maintaining tissue homeostasis through the secretion of cytokines and natural antibodies. We aim to investigate the status of B-1a cells after irradiation, and their role in ameliorating radiation-induced intestinal injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      C57BL\/6 mice were exposed to 12-Gy partial body irradiation (PBI) and B-1a cells in the PerC, spleen and bone marrow were determined by flow cytometry. After 24 hours of PBI, 5\u00d710\n                      <jats:sup>5<\/jats:sup>\n                      B-1a cells were intraperitoneally administered in additional animals. Gut histology, intestinal barrier function, tissue injury markers, and TGF-\u03b2 levels in PerC and gut tissue were assessed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Irradiation induced apoptosis in B-1a cells, resulting in depletion of B-1a cell numbers. Irradiation increased apoptotic cells in the crypts, decreased tight junction protein expression, and enhanced intestinal permeability. Adoptive transfer of B-1a cells significantly ameliorated these changes. The number of TGF-\u03b2-positive B-1a cells in PerC increased after B-1a cell transfer, accompanied by elevated TGF-\u03b2 levels in both PerC and gut tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We demonstrated that B-1a cell numbers are significantly decreased following PBI and that B-1a cell treatment alleviates radiation-induced intestinal injury possibly via the increase in TGF-\u03b2 production.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1761007","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T05:34:23Z","timestamp":1770183263000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["B-1a cells mitigate radiation injury by protecting intestinal barrier 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