{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:22:45Z","timestamp":1771928565626,"version":"3.50.1"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pyroptosis, an inflammatory form of programmed cell death, contributes to intestinal inflammation in inflammatory bowel disease (IBD), but the key cell types and regulatory genes remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We analyzed single-cell RNA-seq data from intestinal mucosa to assess pyroptosis-related gene expression signatures using AUCell, AddModuleScore, and GSVA. High- and low-pyroptosis cells were compared to identify core genes. Findings were validated in bulk peripheral blood transcriptomic data. Machine learning (LASSO, GBM, SVM, Boruta, Random Forest) identified optimal diagnostic genes, and functional enrichment explored biological roles.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Pyroptosis was most active in macrophages, epithelial cells, and neutrophils. Six cell subsets consistently exhibited high pyroptosis. Nineteen core pyroptosis-related genes were identified, 16 of which were upregulated in both mucosal and blood samples. Functional analysis linked these genes to apoptosis and reactive oxygen species pathways. Machine learning highlighted six key diagnostic genes\u2014BASP1, LITAF, NAMPT, PHACTR1, PLAUR, PPIF\u2014with BASP1 showing the strongest performance (AUC = 0.935).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Pyroptosis is highly active in specific immune and epithelial cells in IBD. Six identified genes show potential as non-invasive diagnostic biomarkers, offering insights into disease mechanisms and therapeutic targets.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1761476","type":"journal-article","created":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T06:48:59Z","timestamp":1770187739000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated single-cell and transcriptomic profiling identifies machine-learning\u2013based pyroptosis biomarkers in IBD"],"prefix":"10.3389","volume":"17","author":[{"given":"Yao","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, The People\u2019s Hospital of Wenshan Prefecture, Wenshan","place":["Yunnan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yahui","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal and Hernia Surgery, The First Affiliated Hospital of Kunming Medical University","place":["Kunming, Yunnan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuansen","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal and Hernia Surgery, The First Affiliated Hospital of Kunming Medical University","place":["Kunming, Yunnan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huan","family":"Sai","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, The People\u2019s Hospital of Wenshan Prefecture, Wenshan","place":["Yunnan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal and Hernia Surgery, The First Affiliated Hospital of Kunming Medical University","place":["Kunming, Yunnan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinjiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Breast, Thyroid and Burn Surgery, The People\u2019s Hospital of Wenshan Prefecture","place":["Wenshan, Yunnan, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,2,4]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2015.150","article-title":"The global burden of IBD: from 2015 to 2025","volume":"12","author":"Kaplan","year":"2015","journal-title":"Nat Rev Gastroenterol Hepatol"},{"key":"B2","doi-asserted-by":"publisher","first-page":"S5","DOI":"10.1093\/ibd\/izae081","article-title":"Challenges in IBD Research 2024: Preclinical Human IBD Mechanisms","volume":"30","author":"Ciorba","year":"2024","journal-title":"Inflammation Bowel Dis"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.7150\/thno.71086","article-title":"Pyroptosis 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