{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T07:00:14Z","timestamp":1780470014295,"version":"3.54.1"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:00:00Z","timestamp":1780444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The adequacy of neck dissection following neoadjuvant immunotherapy combined with chemotherapy (NICT) for locally advanced oral squamous cell carcinoma (OSCC) lacks evidence-based benchmarks. This study aimed to define and validate extent-specific lymph node dissection (LND) adequacy thresholds associated with survival outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In a development and validation study, the training cohort comprised 256 consecutive patients with locally advanced OSCC treated with NICT and surgery at Henan Cancer Hospital (2019-2022). An independent cohort of 199 patients from the First Affiliated Hospital of Zhengzhou University (2020-2022) served for validation. Patients were stratified by surgical extent: unilateral (Group Un) or bilateral (Group Bi) neck dissection. Optimal LND thresholds predictive of 3-year overall survival (OS) were determined using restricted cubic splines and maximally selected rank statistics. For Group Un, the threshold (LND-Un) was based on total lymph node yield; for Group Bi, the threshold (LND-Bi) was based on the average yield per side. Survival and safety outcomes were compared between patients meeting (\u201cAdequate\u201d) or not meeting (\u201cInadequate\u201d) these thresholds.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The optimal thresholds were 20 total lymph nodes for unilateral dissections (LND-Un) and 18 nodes per side on average for bilateral dissections (LND-Bi). In the training cohort, inadequate LND was independently associated with worse OS (Group Un: adjusted hazard ratio [aHR] 2.42, 95% CI 1.47-3.99, p&amp;lt;0.001; Group Bi: aHR 2.29, 95% CI 1.20-4.37, p=0.012) and disease-free survival (DFS). Inadequate LND was also linked to a higher risk of major complications in Group Un (adjusted odds ratio 2.15, 95% CI 1.06-4.38, p=0.034). These findings were robustly validated in the external cohort, where the LND-based model demonstrated good discrimination (C-index for OS: 0.71), excellent calibration, and positive net benefit on decision curve analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study establishes and validates stratified, surgical extent-specific thresholds for lymph node dissection adequacy after NICT in OSCC. Achieving a yield of \u226520 nodes in unilateral dissection or an average of \u226518 nodes per side in bilateral dissection is independently associated with significantly improved survival and an acceptable safety profile. These benchmarks provide a tangible, evidence-based guide for surgical quality assessment and decision-making in the post-NICT setting.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1782877","type":"journal-article","created":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T06:00:44Z","timestamp":1780466444000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Stratified lymph node yield thresholds after neoadjuvant immunochemotherapy: a surgical benchmark for survival in oral squamous cell carcinoma"],"prefix":"10.3389","volume":"17","author":[{"given":"Shuai","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Stomatology, The First Affiliated Hospital of Zhengzhou University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunshuang","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Stomatology, The First Affiliated Hospital of Zhengzhou University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yalin","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Stomatology, The First Affiliated Hospital of Zhengzhou University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunzhe","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Stomatology, The First Affiliated Hospital of Zhengzhou University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qigen","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Head and Neck, The Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Stomatology, The First Affiliated Hospital of Zhengzhou University","place":["Zhengzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,3]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2024.155489","article-title":"Comprehensive insights into oral squamous cell carcinoma: Diagnosis, pathogenesis, and therapeutic advances","volume":"261","author":"Jagadeesan","year":"2024","journal-title":"Pathol Res Pract"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.2217\/imt-2023-0174","article-title":"Immunotherapy for head and neck squamous cell carcinoma: current status and perspectives","volume":"16","author":"Daste","year":"2024","journal-title":"Immunotherapy"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1669368","article-title":"Anti-EGFR enhanced neoadjuvant immunotherapy versus neoadjuvant immunochemotherapy for locally advanced oral squamous cell 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