{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:17:08Z","timestamp":1778566628517,"version":"3.51.4"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T00:00:00Z","timestamp":1778544000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004587","name":"Instituto de Salud Carlos III","doi-asserted-by":"publisher","award":["PI22\/01489"],"award-info":[{"award-number":["PI22\/01489"]}],"id":[{"id":"10.13039\/501100004587","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Immune thrombocytopenia is an autoimmune bleeding disorder that is more prevalent among older adults. Ageing itself reduces B-cell counts, a change also observed in patients with ITP. This study examined the B cell profiles of patients with ITP aged over 65 (ITP&amp;gt;65) and aged 65 or under (ITP \u2264 65). These ITP groups were compared with age-matched healthy controls to determine whether the observed differences were due to the disease itself or the effects of ageing.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Blood samples were processed and stained using the EuroFlow 8-colour PIDOT and pre-germinal centre B-cell tubes, following the EuroFlow SOPs for staining cell surface membrane markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Patients with ITP&amp;gt;65, compared with those \u226465, showed reduced immature\/transitional B cell subsets, an increased population of CD21-CD24- na\u00efve B cells, and higher plasma B-cell activating factor levels. Comparison of the ITP \u2264 65 group with the HC \u2264 65 group showed that patients with ITP had a lower B-cell count, but a significant increase in the CD21-CD24- na\u00efve B-cell subset. Patients with ITP&amp;gt;65 had expanded CD21-CD24- and CD21-CD24++ na\u00efve and memory IgMD+ B-cell populations compared to\n                      <jats:italic>the<\/jats:italic>\n                      HC&amp;gt;65 group.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings suggest that ageing induces modifications to the B-cell phenotype that are similar to those observed in patients with ITP, except for the expansion of the CD21-CD24- na\u00efve B-cell subset,\n                      <jats:italic>which appears to be a characteristic of ITP shared with other autoimmune diseases.<\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1791460","type":"journal-article","created":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:45:30Z","timestamp":1778564730000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Age-related and disease-specific changes in B-cell profiles in older adults with immune thrombocytopenia"],"prefix":"10.3389","volume":"17","author":[{"given":"E.","family":"Monz\u00f3n Manzano","sequence":"first","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Herrero Carrasco","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, Severo Ochoa University Hospital","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Acu\u00f1a","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"del Pino Molina","sequence":"additional","affiliation":[{"name":"Clinical Immunology Department, La Paz University Hospital, and Center for Biomedical Network Research on Rare Diseases (CIBERER U767)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. G.","family":"Arias-Salgado","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Mendoza","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Kapur","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Foundation, Research Department, and Amsterdam University Medical Center (UMC) location, Landsteiner Laboratory","place":["Amsterdam, Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Porcelijn","sequence":"additional","affiliation":[{"name":"Sanquin Diagnostic Services, Department of Immunohematology Diagnostics","place":["Sanquin, Amsterdam, Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Mart\u00edn Salces","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. I.","family":"Rivas Pollmar","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"L\u00f3pez-Granados","sequence":"additional","affiliation":[{"name":"Clinical Immunology Department, La Paz University Hospital, and Center for Biomedical Network Research on Rare Diseases (CIBERER U767)","place":["Madrid, Spain"]},{"name":"Lymphocyte Pathophysiology in Immunodeficiencies Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Jim\u00e9nez Yuste","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]},{"name":"Faculty of Medicine, Universidad Aut\u00f3noma de Madrid","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Gasior Kabat","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Ram\u00edrez Mart\u00edn","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Universidad Aut\u00f3noma de Madrid","place":["Madrid, Spain"]},{"name":"Geriatrics Department, La Paz University Hospital \u2013Ageing and Fragility in Elderly Persons Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Butta","sequence":"additional","affiliation":[{"name":"Haematology and Haemotherapy Department, La Paz University Hospital, Bleeding and Haemostasis Disorders Group-Health Research Institute of the La Paz University Hospital (IdiPAZ)","place":["Madrid, Spain"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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