{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T06:06:43Z","timestamp":1784786803122,"version":"3.55.0"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T00:00:00Z","timestamp":1784764800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Zika virus (ZIKV) causes congenital disease and neurological complications, yet no approved vaccines or antivirals are available. Integrin \u03b24 (ITGB4) has been identified as an entry receptor for ZIKV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Recombinant human ITGB4 ectodomain was expressed using a baculovirus-insect cell system and used to immunize BALB\/c mice. Hybridoma screening identified monoclonal antibody 7C3. Binding affinity to ITGB4 was determined by biolayer interferometry. Epitope relationships between 7C3 and the previously reported anti-ITGB4 antibody 13H10 were assessed by competitive binding assay. Antiviral activity was evaluated in four cell lines by RT-qPCR quantification of cell-associated ZIKV RNA after antibody pretreatment and viral challenge.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      7C3 bound ITGB4 with picomolar affinity (K\n                      <jats:sub>D<\/jats:sub>\n                      =30.9 pM) and high specificity. Competitive binding showed that 7C3 and 13H10 (K\n                      <jats:sub>D<\/jats:sub>\n                      =7.98 pM) recognize non-overlapping epitopes on ITGB4. Pretreatment with 7C3 significantly reduced cell-associated ZIKV RNA in all four cell lines compared with PBS and isotype controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>\n                      A second, independently generated anti-ITGB4 antibody with picomolar affinity can robustly block ZIKV entry across multiple cell types, including SY5Y neuroblastoma cells relevant to congenital neuropathology. The non-overlapping epitopes of 7C3 and 13H10 suggest potential for combination strategies to enhance ITGB4 blockade. High germline identity of the 7C3 variable regions (V\n                      <jats:sub>H<\/jats:sub>\n                      =94.46%, V\n                      <jats:sub>L<\/jats:sub>\n                      =95.82%) supports future humanization. These findings establish 7C3 as a host-directed antiviral candidate and provide a basis for ITGB4-targeting approaches against congenital ZIKV disease.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1796913","type":"journal-article","created":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T05:40:31Z","timestamp":1784785231000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Generation and characterization of an anti ITGB4 monoclonal antibody that blocks Zika virus entry"],"prefix":"10.3389","volume":"17","author":[{"given":"Xiaodong","family":"Han","sequence":"first","affiliation":[{"name":"College of Life Sciences, Inner Mongolia Agriculture University","place":["Hohhot, Inner Mongolia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Inner Mongolia Agriculture University","place":["Hohhot, Inner Mongolia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuqian","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Inner Mongolia Agriculture University","place":["Hohhot, Inner Mongolia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yancun","family":"Gong","sequence":"additional","affiliation":[{"name":"Inner Mongolia Animal Disease Prevention and Control Center","place":["Hohhot, Inner Mongolia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Shen","sequence":"additional","affiliation":[{"name":"Inner Mongolia Animal Disease Prevention and Control Center","place":["Hohhot, Inner Mongolia, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Song","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Chinese Academy of Sciences","place":["Beijing, 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