{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T06:13:23Z","timestamp":1778739203781,"version":"3.51.4"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T00:00:00Z","timestamp":1778716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Immunometabolic remodeling is central to sepsis, yet robust glycolysis-associated biomarkers and their cell-type context remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We integrated peripheral blood scRNA-seq (GSE175453) and a whole-blood microarray cohort (GSE100159). Glycolysis activity was scored by AUCell (HALLMARK_GLYCOLYSIS), hub genes were prioritized by LASSO\/random forest\/Boruta, communication was inferred by ligand\u2013receptor analysis, and qRT-PCR was performed in CLP vs control mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Sepsis showed myeloid predominance and increased glycolysis scores, most evident in monocytes and plasma cells. Five candidates (GLRX, MDH1, MDH2, TGFBI, COPB2) displayed good discriminatory performance in bulk data; TGFBI was monocyte-enriched and centrally positioned in a dense communication network with B cells\/plasma cells\/neutrophils. qRT-PCR confirmed a significant between-group difference for TGFBI in the CLP model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings link enhanced glycolysis-associated programs to a monocyte-centered TGFBI communication pattern and prioritize TGFBI as a candidate biomarker for further validation in sepsis.<\/jats:p>\n                  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