{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:20:36Z","timestamp":1781846436747,"version":"3.54.5"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    Ovarian cancer is characterized by a profoundly immunosuppressive tumor microenvironment, which has limited the clinical success of immunotherapy approaches. Antigen-based nanovaccines represent a promising strategy to enhance antitumor immunity by improving antigen delivery and immune activation. In this study, we evaluated carbon nanotubes (CNTs) bioconjugated with a peptide derived from tumor-associated antigen fucosyltransferase 4 (FUT4) as an immunomodulating platform in an immunocompetent ovarian cancer mouse model. FUT4 expression was confirmed in the ID8-Defb29\/Vegf-a (ID8DVLuc) ovarian cancer model using flow cytometry and confocal microscopy. C57BL\/6 mice were immunized with free peptide combined with a conventional adjuvant (PEP37 Adj), FUT4 peptide\u2013conjugated CNTs (\n                    <jats:italic>f<\/jats:italic>\n                    -CNTs), or\n                    <jats:italic>f<\/jats:italic>\n                    -CNTs combined with adjuvant (\n                    <jats:italic>f<\/jats:italic>\n                    -CNTs Adj), and compared with nonimmunized controls. Immune profiling revealed increased leukocyte infiltration and enrichment of M1-like macrophages, dendritic cells, and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, along with a reduction in regulatory T cells.\n                    <jats:italic>f<\/jats:italic>\n                    -CNT immunization also induced enhanced humoral responses, increased splenocyte proliferation upon\n                    <jats:italic>ex vivo<\/jats:italic>\n                    peptide restimulation, and increased\n                    <jats:italic>ex vivo<\/jats:italic>\n                    CD8\n                    <jats:sup>+<\/jats:sup>\n                    T-cell\u2013mediated cytotoxicity. Exploratory RNA sequencing of ascites-derived cells from\n                    <jats:italic>f<\/jats:italic>\n                    -CNT\u2013immunized mice suggested the upregulation of genes associated with antigen processing and presentation, CD8\n                    <jats:sup>+<\/jats:sup>\n                    T-cell activation, and Th1-type immune responses. Immunized mice exhibited reduced ascites accumulation, delayed disease progression, as assessed by bioluminescence imaging, and prolonged survival, with the most pronounced effects observed in the\n                    <jats:italic>f<\/jats:italic>\n                    -CNT group. Based on these findings, we propose that FUT4 peptide\u2013conjugated CNTs function as an antigen-based nanoplatform that enhances antitumor immune responses in a murine ovarian cancer model. While additional studies are needed to further validate antigen specificity and therapeutic robustness, this work supports the potential of CNT-based nanocarriers as immune-modulating platforms for ovarian cancer immunotherapy.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2026.1821727","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T05:43:29Z","timestamp":1779169409000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Fucosyltransferase 4-derived peptide bioconjugates on carbon nanotubes enhance antitumor immunity in an ovarian cancer mouse model"],"prefix":"10.3389","volume":"17","author":[{"given":"J. J.","family":"Guzm\u00e1n-Mendoza","sequence":"first","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular. Centro de Investigaci\u00f3n y de Estudios Avanzados","place":["Ciudad de M\u00e9xico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"S\u00e1nchez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Facultad de Ciencias Qu\u00edmicas, Universidad Aut\u00f3noma de Chihuahua","place":["Chihuahua, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Velarde-Calder\u00f3n","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular. Centro de Investigaci\u00f3n y de Estudios Avanzados","place":["Ciudad de M\u00e9xico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. M.","family":"Gonz\u00e1lez-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Nano y Biomateriales Dentales, Facultad de Estudios Superiores Iztacala, Universidad Nacional Aut\u00f3noma de M\u00e9xico","place":["Ciudad de M\u00e9xico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Garc\u00eda-Campuzano","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular. Centro de Investigaci\u00f3n y de Estudios Avanzados","place":["Ciudad de M\u00e9xico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Jim\u00e9nez-Bernal","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular. Centro de Investigaci\u00f3n y de Estudios Avanzados","place":["Ciudad de M\u00e9xico, Mexico"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. U.","family":"L\u00f3pez-Bail\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Integrative Immunobiology, Duke University School of Medicine","place":["Durham, NC, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Talam\u00e1s-Rohana","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular. 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