{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T21:05:52Z","timestamp":1779915952473,"version":"3.53.1"},"reference-count":35,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T00:00:00Z","timestamp":1779926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:title>Objective<\/jats:title>\n                  <jats:p>\n                    BufeiJianpi Mixture (BFJPM) is a modified herbal formula developed from long-term clinical practice for the management of allergic constitution in children. To evaluate the therapeutic efficacy of BFJPM in an OVA-induced murine model of food allergy and to investigate its effects on the gut microbiota through integrated animal experiments, 16S rRNA gene sequencing, and network pharmacology. Methods: Sixty 3-week-old female BALB\/c mice were randomly assigned to six groups: negative control (NC), FA model, cetirizine, BFJPM-L, BFJPM-M, and BFJPM-H. An OVA-induced food allergy model was established. Therapeutic efficacy was assessed using allergy symptom scores, the V\/C ratio of the small intestinal mucosa, and serum total IgE levels. Gut microbiota profiles were analyzed by 16S rRNA sequencing of intestinal contents. PICRUSt2 was used to predict microbial functional profiles, and network pharmacology was applied to identify key targets and pathways involved in BFJPM-mediated intervention. Results: BFJPM reduced allergy symptom scores and serum total IgE levels and improved the V\/C ratio in FA mice. NMDS analysis showed significant differences in gut microbiota composition among groups (stress = 0.141). BFJPM increased microbial richness and altered community structure, with enrichment of\n                    <jats:italic>Actinobacteria<\/jats:italic>\n                    and\n                    <jats:italic>Candidatus Arthromitus<\/jats:italic>\n                    . Among the differential taxa,\n                    <jats:italic>Saccharopolyspora<\/jats:italic>\n                    emerged as the most promising genus associated with BFJPM intervention and was negatively correlated with serum IgE levels. Integrated network pharmacology and microbial functional prediction identified IL6, TNF, and IL10 as major targets of BFJPM in FA. The African trypanosomiasis pathway was identified as a shared pathway linking BFJPM, FA, and gut microbiota-associated functional alterations. Conclusion: BFJPM alleviated food allergy symptoms, reduced serum IgE levels, and improved intestinal mucosal integrity in OVA-induced FA mice. These effects were accompanied by remodeling of the gut microbiota.\n                    <jats:italic>Saccharopolyspora<\/jats:italic>\n                    may represent a microbial feature associated with BFJPM treatment. The African trypanosomiasis pathway may indicate a potential point of convergence between BFJPM-regulated host pathways and microbiota-associated functional changes. However, the causal relationship between microbiota remodeling and therapeutic efficacy remains to be established.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2026.1827925","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T20:10:20Z","timestamp":1779912620000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["BFJPM ameliorates OVA-induced food allergy in a murine model accompanied by gut microbiota remodeling"],"prefix":"10.3389","volume":"17","author":[{"given":"Xue","family":"Yang","sequence":"first","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine","place":["Changsha, China"]},{"name":"Hunan University of Chinese Medicine","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyi","family":"Peng","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine","place":["Changsha, China"]},{"name":"Hunan University of Chinese Medicine","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rao","family":"Hu","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine","place":["Changsha, China"]},{"name":"Hunan University of Chinese Medicine","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Li","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine","place":["Changsha, China"]},{"name":"Hunan University of Chinese Medicine","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Jiang","sequence":"additional","affiliation":[{"name":"The First Hospital of Hunan University of Chinese Medicine","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,5,28]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1673-8705.2022.06.012","article-title":"Mechanism of immune sensitization and immunotherapy of food allergy","volume":"16","author":"Tong","year":"2022","journal-title":"Chin J Allergy Clin Immunol"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1111\/pai.13490","article-title":"Time trends of childhood food allergy in China: three cross-sectional surveys in 1999, 2009, and 2019","volume":"32","author":"Ma","year":"2021","journal-title":"Pediatr Allergy Immunol Off Publ Eur Soc Pediatr Allergy Immunol"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3390\/nu14235155","article-title":"Microbiome therapeutics for food allergy","volume":"14","author":"Chernikova","year":"2022","journal-title":"Nutrients"},{"key":"B4","doi-asserted-by":"publisher","DOI":"10.13381\/j.cnki.cjm.2006.02.014","article-title":"The analysis of intestinal microflora of infants with food allergy and healthy infants","volume":"18","author":"Wang","year":"2006","journal-title":"Chin J Microecol"},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1016\/j.aller.2015.01.013","article-title":"Intestinal microbiota and allergic diseases: a systematic review","volume":"44","author":"Melli","year":"2016","journal-title":"Allergol Immunopathol (Madr)"},{"key":"B6","doi-asserted-by":"publisher","DOI":"10.13343\/j.cnki.wsxb.20230802","article-title":"Research progress in the relationship between gut microbiota and food allergy in children","volume":"64","author":"Yang","year":"2024","journal-title":"Acta Microbiol Sin"},{"key":"B7","doi-asserted-by":"publisher","first-page":"58","DOI":"10.3969\/j.issn.1674-070X.2012.11.017.058.03","article-title":"Effects of staged TCM differentiation and treatment on the serum IgE and ECP of cough variant asthma children","volume":"32","author":"Shu","year":"2012","journal-title":"J TCM Univ Hunan"},{"key":"B8","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2020.109777","article-title":"Polysaccharides from Atractylodes macrocephala koidz. 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