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Immunol."],"abstract":"<jats:p>\n                    The study of cancer in dogs can provide valuable insights into the basic biology and the development of novel cancer treatments as veterinary cases develop spontaneously, exhibit heterogeneity and closely match the biology of human cancers. Here we have isolated and characterized cell lines derived from spontaneous oral melanomas from companion dogs and subjected these cells to adenovirus-mediated gene transfer both\n                    <jats:italic>in vitro<\/jats:italic>\n                    and\n                    <jats:italic>in vivo<\/jats:italic>\n                    . Two newly established cell lines, BAN and TIG, were confirmed to be oncogenically transformed, as they bypassed G1 arrest under serum starvation and were tumorigenic in nude mice. These cell lines harbor wild-type p53, which, in response to treatment with doxorubicin or Nutlin-3, promoted the expression of well-known p53 target genes (CDKN1A, MDM2). The established cell lines are permissive to transduction with RGD-modified non-replicating adenoviral vectors and allowed reporter gene activity from p53-responsive promoter vectors controlled. Treatment with adenoviral vectors encoding canine p14\n                    <jats:sup>ARF<\/jats:sup>\n                    and interferon-\u03b2 (IFN\u03b2) resulted in cell death with liberation of immunogenic cell death markers\n                    <jats:italic>in vitro<\/jats:italic>\n                    and reduction of tumor progression when subcutaneous tumors in nude mice were treated with\n                    <jats:italic>in situ<\/jats:italic>\n                    gene therapy. These results indicate that adenovirus-mediated delivery of p14ARF and IFN\u03b2 is effective in a canine model of oral melanoma, supporting the feasibility of applying comparative oncology approaches to the development of this gene therapy strategy.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2026.1835389","type":"journal-article","created":{"date-parts":[[2026,5,28]],"date-time":"2026-05-28T06:17:38Z","timestamp":1779949058000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":1,"title":["Adenovirus mediated gene therapy in cell lines derived from canine oral melanoma"],"prefix":"10.3389","volume":"17","author":[{"given":"Otavio Augusto","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Vetores Virais (LVV), Centro de Investiga\u00e7\u00e3o Translacional em Oncologia (CTO)\/LIM24, Instituto do C\u00e2ncer do Estado de S\u00e3o Paulo (ICESP), Faculdade de Medicina, Universidade de S\u00e3o Paulo (FMUSP); Comprehensive Center for Precision Oncology (C2PO), Universidade de S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda","family":"Antunes","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Vetores Virais (LVV), Centro de Investiga\u00e7\u00e3o Translacional em Oncologia (CTO)\/LIM24, Instituto do C\u00e2ncer do Estado de S\u00e3o Paulo (ICESP), Faculdade de Medicina, Universidade de S\u00e3o Paulo (FMUSP); Comprehensive Center for Precision Oncology (C2PO), Universidade de S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gissele Rolemberg Oliveira","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Vetores Virais (LVV), Centro de Investiga\u00e7\u00e3o Translacional em Oncologia (CTO)\/LIM24, Instituto do C\u00e2ncer do Estado de S\u00e3o Paulo (ICESP), Faculdade de Medicina, Universidade de S\u00e3o Paulo (FMUSP); Comprehensive Center for Precision Oncology (C2PO), Universidade de S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianca Naomi","family":"Niitsuma","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Vetores Virais (LVV), Centro de Investiga\u00e7\u00e3o Translacional em Oncologia (CTO)\/LIM24, Instituto do C\u00e2ncer do Estado de S\u00e3o Paulo (ICESP), Faculdade de Medicina, Universidade de S\u00e3o Paulo (FMUSP); Comprehensive Center for Precision Oncology (C2PO), Universidade de S\u00e3o Paulo","place":["S\u00e3o Paulo, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Carlos dos Santos","family":"da Luz","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Vetores Virais (LVV), Centro de Investiga\u00e7\u00e3o Translacional em Oncologia (CTO)\/LIM24, Instituto do C\u00e2ncer do Estado de S\u00e3o Paulo 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