{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T08:34:14Z","timestamp":1782894854660,"version":"3.54.5"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Abdominal aortic aneurysm (AAA) is a life-threatening vascular disease characterized by immune cell infiltration and vascular remodeling. B cells have been implicated in AAA pathogenesis, yet their specific roles and molecular mediators remain incompletely understood. This study aimed to investigate the immune microenvironment of AAA and elucidate the functional role of LUM in B cells using integrated multi-omics and experimental approaches.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We integrated single-cell RNA sequencing (scRNA-seq) and bulk transcriptome data from GEO datasets (GSE183464, GSE226492). Key computational analyses included cell clustering, trajectory inference (CytoTRACE2, Monocle2), cell communication (CellChat), and machine learning-based feature selection (LASSO and SVM). Experimentally, primary human B cells were isolated and subjected to lentivirus-mediated LUM knockdown or overexpression, followed by Transwell co-culture with primary human aortic vascular smooth muscle cells (VSMCs). LUM expression in B cells and plasma was further validated in clinical samples, and its correlation with aneurysm diameter was analyzed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Single-cell analysis identified B cells as a significantly altered immune population in AAA with enhanced communication to VSMCs. CD79A and LUM were identified as key signature genes in B cells. LUM was upregulated at both mRNA and protein levels in AAA tissues and specifically enriched in B cells. Functional experiments demonstrated that LUM expression in B cells promoted VSMC phenotypic switching toward a synthetic phenotype (upregulated OPN and downregulated contractile markers). Knockdown of LUM in B cells attenuated this effect, whereas overexpression enhanced it. Clinically, LUM protein levels in B cells and plasma increased with larger aneurysm diameter and positively correlated with maximum AAA diameter.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study reveals that LUM\n                      <jats:sup>+<\/jats:sup>\n                      B cells play a critical role in AAA by promoting VSMC phenotypic switching. The combination of single-cell transcriptomics, functional validation, and clinical correlation establishes LUM in B cells as both a mechanistic contributor and a potential biomarker for AAA severity. These findings provide new insights into B cell-mediated vascular remodeling and highlight LUM as a promising therapeutic target.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1836487","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T06:35:32Z","timestamp":1782887732000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Single-cell sequencing combined with transcriptome analysis unravels LUM+ B cells as key drivers in abdominal aortic aneurysm"],"prefix":"10.3389","volume":"17","author":[{"given":"Hanqing","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Surgery, Zhongnan Hospital of Wuhan University","place":["Wuhan, China"]},{"name":"Hubei Provincial Engineering Research Center of Minimally Invasive Cardiovascular Surgery","place":["Wuhan, China"]},{"name":"Wuhan Clinical Research Center for Minimally Invasive Treatment of Structural Heart Disease","place":["Wuhan, China"]},{"name":"State Key Laboratory of Metabolism and Regulation inComplex Organisms","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqi","family":"Peng","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Zhongnan Hospital of Wuhan University","place":["Wuhan, China"]},{"name":"Hubei Provincial Engineering Research Center of Minimally Invasive Cardiovascular Surgery","place":["Wuhan, China"]},{"name":"Wuhan Clinical Research Center for Minimally Invasive Treatment of Structural Heart Disease","place":["Wuhan, China"]},{"name":"State Key Laboratory of Metabolism and Regulation inComplex Organisms","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liman","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, West China Hospital of Sichuan University","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Zhongnan Hospital of Wuhan University","place":["Wuhan, China"]},{"name":"Hubei Provincial Engineering Research Center of Minimally Invasive Cardiovascular Surgery","place":["Wuhan, China"]},{"name":"Wuhan Clinical Research Center for Minimally Invasive Treatment of Structural Heart Disease","place":["Wuhan, China"]},{"name":"State Key Laboratory of Metabolism and Regulation inComplex Organisms","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Taikang Tongji (Wuhan) Hospital","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Zhongnan Hospital of Wuhan University","place":["Wuhan, China"]},{"name":"Hubei Provincial Engineering Research Center of Minimally Invasive Cardiovascular Surgery","place":["Wuhan, China"]},{"name":"Wuhan Clinical Research Center for Minimally Invasive Treatment of Structural Heart Disease","place":["Wuhan, China"]},{"name":"State Key Laboratory of Metabolism and Regulation inComplex Organisms","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengwei","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery, Zhongnan Hospital of Wuhan University","place":["Wuhan, China"]},{"name":"Hubei Provincial Engineering Research Center of Minimally Invasive Cardiovascular Surgery","place":["Wuhan, China"]},{"name":"Wuhan Clinical Research Center for Minimally Invasive Treatment of Structural Heart Disease","place":["Wuhan, China"]},{"name":"State Key Laboratory of Metabolism and Regulation 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