{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T08:20:31Z","timestamp":1782894031220,"version":"3.54.5"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"\n Introduction<\/jats:title>\n Platelets promote hemostasis and modulate different inflammatory and immunological processes. Recently, it was reported that platelets interact with pancreatic beta cells to increase insulin secretion. Inflammatory conditions and a lack of sufficient insulin secretion drive the pathogenesis of type 2 diabetes (T2D) in the context of obesity. However, the specific role of platelets during the development of obesity-related T2D has not yet been investigated.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Thrombopoietin receptor knockout (\n \n MPL\n -\/-<\/jats:sup>\n <\/jats:italic>\n ) mice displaying constant thrombocytopenia (approx. 90 % reduction in platelet count) or wild type (WT) mice were fed either a high-fat diet (HFD) or a control diet (CD) for 19 weeks, starting at 8 weeks of age. The mice were metabolically characterized at the end of the feeding period (metabolic caging, glucose and insulin tolerance testing, body and organ weight measurements, and gene expression analyses). Pancreatic tissue was histologically assessed via immunofluorescence, and circulating platelets were characterized by flow cytometry.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n After HFD feeding, obese\n \n MPL\n -\/-<\/jats:sup>\n <\/jats:italic>\n mice showed a significantly improved glucose tolerance compared to WT mice. At the same time,\n \n MPL\n -\/-<\/jats:sup>\n <\/jats:italic>\n mice tended to exhibit a more pronounced insulin resistance. The weight gain of\n \n MPL\n -\/-<\/jats:sup>\n <\/jats:italic>\n and WT mice did not differ significantly after 19 weeks of feeding, but body weight was higher in\n \n MPL\n -\/-<\/jats:sup>\n <\/jats:italic>\n between weeks 4 and 12. Likewise, the weight of adipose tissue depots was similar between both genotypes at the end of the study. In metabolic cages, obese\n \n MPL\n -\/-<\/jats:sup>\n <\/jats:italic>\n mice showed less physical activity than WT, while other parameters like O\n 2<\/jats:sub>\n -consumption and heat dissipation were similar between the two groups. The expression of genes involved in glucose metabolism in muscle, liver, and epididymal adipose tissue did not show relevant differences between\n \n MPL\n -\/-<\/jats:sup>\n <\/jats:italic>\n and WT mice. At the end of the study, a significantly higher insulin concentration was measured in the circulation of obese\n \n MPL\n -\/-<\/jats:sup>\n <\/jats:italic>\n mice. This was accompanied by a higher proportion of pancreatic beta cells in the total analyzed tissue area.\n <\/jats:p>\n <\/jats:sec>\n \n Discussion<\/jats:title>\n These results suggests that thrombocytopenia protects pancreatic beta cells in an obesity mouse model of T2D, thereby preserving insulin secretion and improving glucose tolerance.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1854992","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T06:19:17Z","timestamp":1782886757000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Reduced platelet count in mice protects against glucose intolerance and beta cell loss during a long-term high-fat diet"],"prefix":"10.3389","volume":"17","author":[{"given":"Niklas","family":"Burkhard","sequence":"first","affiliation":[{"name":"Cardiology and Angiology, Medical Center \u2013 University of Freiburg, Faculty of Medicine, University 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AngioScience (ECAS), German Centre for Cardiovascular Research (DZHK) partner site Heidelberg\/Mannheim, and Centre for Cardiovascular Acute Medicine Mannheim (ZKAM), Medical Centre Mannheim and Medical Faculty Mannheim, Heidelberg University","place":["Mannheim, Germany"]},{"name":"Department of Pediatrics, Harvard Medical School","place":["Boston, MA, United States"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolin","family":"Mogler","sequence":"additional","affiliation":[{"name":"Institute of Pathology, School of Medicine and Health, Technical University Munich","place":["Munich, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Stallmann","sequence":"additional","affiliation":[{"name":"Cardiology and Angiology, Medical Center \u2013 University of Freiburg, Faculty of Medicine, University of Freiburg","place":["Freiburg, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Mangin","sequence":"additional","affiliation":[{"name":"Universit\u00e9 de Strasbourg, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Etablissement Fran\u00e7ais du Sang Grand-Est, Unit\u00e9 Mixte de Recherche-S1255, F\u00e9d\u00e9ration de Medecine Translationnelle de Strasbourg","place":["Strasbourg, France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krystin","family":"Krauel","sequence":"additional","affiliation":[{"name":"Cardiology, Haemostaseology, and Medical Intensive Care, Medical Centre Mannheim, Medical Faculty Mannheim, Heidelberg University","place":["Mannheim, Germany"]},{"name":"European Center for AngioScience (ECAS), German Centre for Cardiovascular Research (DZHK) partner site Heidelberg\/Mannheim, and Centre for Cardiovascular Acute Medicine Mannheim (ZKAM), Medical Centre Mannheim and Medical Faculty Mannheim, Heidelberg University","place":["Mannheim, Germany"]},{"name":"Helmholtz-Institute for Translational AngioCardioScience (HI-TAC) of the Max Delbr\u00fcck Center for Molecular Medicine in the Helmholtz Association (MDC) at Heidelberg University","place":["Mannheim, Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Duerschmied","sequence":"additional","affiliation":[{"name":"Cardiology, Haemostaseology, and Medical Intensive Care, Medical Centre Mannheim, Medical Faculty Mannheim, Heidelberg University","place":["Mannheim, Germany"]},{"name":"European Center for AngioScience (ECAS), German Centre for Cardiovascular Research (DZHK) partner site Heidelberg\/Mannheim, and Centre for Cardiovascular Acute Medicine Mannheim (ZKAM), Medical Centre Mannheim and Medical Faculty Mannheim, Heidelberg University","place":["Mannheim, Germany"]},{"name":"Helmholtz-Institute for Translational AngioCardioScience (HI-TAC) of the Max Delbr\u00fcck Center for Molecular 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