{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:15:45Z","timestamp":1788419745662,"version":"build-2803163510"},"reference-count":66,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T00:00:00Z","timestamp":1788393600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    causes severe, often multidrug-resistant lung infections, especially in individuals with cystic fibrosis, highlighting vaccination as a promising preventive approach. This study evaluated intranasal and intradermal immunization with a D-glutamate auxotrophic\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    strain (PAO1 \u0394\n                    <jats:italic>murI<\/jats:italic>\n                    ) and two of its derivatives, one with additional D-alanine auxotrophy and another exhibiting reduced lipopolysaccharide-associated toxicity, in C57BL\/6 mice using both acute and chronic lung infection models. Mice received two or three doses at various intervals, and safety was assessed through clinical and weight monitoring. A high-dose, 2-administration intranasal regimen protected against acute infection but induced transient weight loss, while a lower-dose, 3-administration regimen minimized toxicity. Immunized mice exhibited robust IL-17A production and increased IFN-\u03b3 responses. Following challenge with mucoid strains, vaccinated mice showed reduced lung bacterial loads, suggesting a partial protection against chronic infection. These findings support the potential of auxotrophic live vaccines to mitigate disease severity in patients with cystic fibrosis.\n                  <\/jats:p>","DOI":"10.3389\/fimmu.2026.1862061","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:43:35Z","timestamp":1788417815000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Live auxotrophic vaccines contribute to protection against chronic lung infections caused by Pseudomonas aeruginosa in C57BL\/6 mice"],"prefix":"10.3389","volume":"17","author":[{"given":"V\u00edctor","family":"Fuentes-Valverde","sequence":"first","affiliation":[{"name":"Servicio de Microbiolog\u00eda Cl\u00ednica and Grupo de Investigaci\u00f3n en Microbiolog\u00eda, Instituto de Investigaci\u00f3n Biom\u00e9dica de A Coru\u00f1a (INIBIC), Complexo Hospitalario Universitario de A Coru\u00f1a (CHUAC), Servizo Galego de Sa\u00fade (SERGAS), Universidade 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