{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:01:25Z","timestamp":1790834485437,"version":"4.1.0"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To investigate the effects of\n                      <jats:italic>Lycium barbarum<\/jats:italic>\n                      polysaccharides (LBPs) on the methylation of the Suppressor of Cytokine Signalling-1 (SOCS-1) gene and the maturation and migration of human monocyte-derived dendritic cells (DCs)\n                      <jats:italic>in vitro<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Human monocyte-derived DCs were generated using recombinant human GM-CSF and interleukin-4 (IL-4) and treated with various concentrations of LBPs (50, 100, and 200 mg\/L). SOCS-1 methylation and relative gene expression were analyzed by methylation-specific quantitative PCR (qMSP) and quantitative real-time PCR, respectively. DC surface markers were assessed by flow cytometry. Cytokine (IL-12 and IL-10) secretion was measured by enzyme-linked immunosorbent assay (ELISA). JAK2, STAT3 and STAT5 phosphorylation were assessed by Western Blot Analysis. The ability of LBPs-treated DCs to stimulate lymphocyte proliferation was evaluated in a mixed lymphocyte reaction (MLR). DC migration was tested using a Transwell assay toward MIP-3\u03b2, and CCR7 expression was quantified by real-time PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>LBPs treatment upregulated SOCS-1 gene methylation and increased the expression of maturation markers (CD80, CD83, HLA-DR) and IL-12 production in a dose-dependent manner. Conversely, SOCS-1 expression and IL-10 secretion were downregulated. Furthermore, LBPs-treated DCs enhanced allogeneic lymphocyte proliferation. A significant increase in DC migration toward MIP-3\u03b2 and CCR7 expression was observed in the 200 mg\/L LBPs group compared to the control.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These findings indicate that LBPs are associated with increased SOCS-1 methylation and enhanced maturation and migratory capacity of human monocyte-derived DCs\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Notably, to our knowledge, this is the first report demonstrating that a plant-derived polysaccharide can modulate SOCS-1 gene methylation, suggesting a potential epigenetic mechanism underlying the immunomodulatory activity of LBPs.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1921722","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T05:41:40Z","timestamp":1790833300000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Lycium barbarum polysaccharides modulate SOCS-1 gene methylation and promote the maturation and migration of human monocyte-derived dendritic cells in vitro"],"prefix":"10.3389","volume":"17","author":[{"given":"Wei","family":"Pan","sequence":"first","affiliation":[{"name":"Division of Education and Research, Changzhou Traditional Chinese Medicine Hospital Affiliated to Nanjing University of Chinese 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