{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:55:25Z","timestamp":1790916925762,"version":"4.1.0"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>5-Aminosalicylic acid (5-ASA) is a first-line therapy for ulcerative colitis, but whether dosing timing influences its therapeutic efficacy remains unclear. This study aims to investigate the chronopharmacological effects of 5-ASA in experimental colitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A dextran sulfate sodium (DSS)-induced colitis model was used to compare the therapeutic effects of 5-ASA administered at different Zeitgeber times. Colonic proteomic profiling was performed to identify circadian-responsive proteins associated with differential drug efficacy. Intestinal epithelial-specific\n                      <jats:italic>Bmal1<\/jats:italic>\n                      knockout mice were used to determine whether the circadian clock regulates the chronopharmacological response to 5-ASA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      5-ASA showed marked time-dependent efficacy in DSS-induced colitis, with greater protection at ZT14 than at ZT2. Proteomic analysis identified ferritin heavy chain 1 (FTH1) as a circadian-responsive protein associated with differential treatment outcomes. 5-ASA suppressed FTH1 expression, whereas FTH1 overexpression attenuated anti-inflammatory effects of 5-ASA. Moreover, intestinal epithelial-specific deletion of\n                      <jats:italic>Bmal1<\/jats:italic>\n                      largely abolished diurnal variation in both FTH1 expression and 5-ASA efficacy, indicating that the intestinal clock coordinates the time-dependent response to 5-ASA.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings identify FTH1 as a circadian-responsive factor linked to the time-dependent efficacy of 5-ASA and support circadian-guided optimization of 5-ASA administration as a potential strategy for improving UC treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fimmu.2026.1936952","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:37:57Z","timestamp":1790915877000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Chronopharmacological effects of 5-aminosalicylic acid in the treatment of experimental colitis in mice"],"prefix":"10.3389","volume":"17","author":[{"given":"Yulin","family":"Wen","sequence":"first","affiliation":[{"name":"Chinese Medicine Guangdong Laboratory (Hengqin), State Key Laboratory of Traditional Chinese Medicine Syndrome, Key Laboratory of Chinese Medicinal Resource from Lingnan, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuangye","family":"Li","sequence":"additional","affiliation":[{"name":"Chinese Medicine Guangdong Laboratory (Hengqin), State Key Laboratory of Traditional Chinese Medicine Syndrome, Key Laboratory of Chinese Medicinal Resource from Lingnan, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jincan","family":"Jian","sequence":"additional","affiliation":[{"name":"Chinese Medicine Guangdong Laboratory (Hengqin), State Key Laboratory of Traditional Chinese Medicine Syndrome, Key Laboratory of Chinese Medicinal Resource from Lingnan, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Liu","sequence":"additional","affiliation":[{"name":"Chinese Medicine Guangdong Laboratory (Hengqin), State Key Laboratory of Traditional Chinese Medicine Syndrome, Key Laboratory of Chinese Medicinal Resource from Lingnan, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuai","family":"Wang","sequence":"additional","affiliation":[{"name":"Chinese Medicine Guangdong Laboratory (Hengqin), State Key Laboratory of Traditional Chinese Medicine Syndrome, Key Laboratory of Chinese Medicinal Resource from Lingnan, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haomeng","family":"Wu","sequence":"additional","affiliation":[{"name":"Chinese Medicine Guangdong Laboratory (Hengqin), State Key Laboratory of Traditional Chinese Medicine Syndrome, Key Laboratory of Chinese Medicinal Resource from Lingnan, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"B1","article-title":"\u201cChronotherapy\u201d","volume-title":"Handbook of Clinical Neurology. 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