{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:57:16Z","timestamp":1790949436535,"version":"4.1.0"},"reference-count":38,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Immunol."],"abstract":"<jats:p>Hedysarum polysaccharide (HPS), the major bioactive constituent of Radix Hedysari, possesses anti-inflammatory activity; however, few studies have explored its application for ulcerative colitis (UC). In this study, HPS-80 was extracted using combined complex-enzyme hydrolysis and ultrasonication. We further evaluated its preventive effects and underlying mechanisms in a dextran sulfate sodium (DSS)-induced UC model in C57BL\/6 mice. Results showed HPS-80 significantly improved DSS-induced UC symptoms (weight loss, hematochezia, colon shortening) and reduced Disease Activity Index (DAI). Mechanistically, it inhibited pro-inflammatory factors Tumor Necrosis Factor-alpha(TNF-\u03b1),Interleukin-6, (IL-6), alleviated oxidative stress, upregulated tight junction proteins occludin, claudin-1(ZO-1), and modulated intestinal microbiota (suppressed harmful bacteria like Bacteroidota, enriched beneficial ones like Firmicutes and Akkermansia). Conclusion: HPS-80 exerts protective effects against UC via anti-inflammatory, antioxidant and gut-microbiota-regulating capacities. It is a promising candidate agent for UC prevention.<\/jats:p>","DOI":"10.3389\/fimmu.2026.1944924","type":"journal-article","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T13:31:36Z","timestamp":1790947896000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Polysaccharides from Radix Hedysari exhibited preventive effects against DSS-induced ulcerative colitis in C57BL\/6 mice"],"prefix":"10.3389","volume":"17","author":[{"given":"Lujie","family":"Ma","sequence":"first","affiliation":[{"name":"Key Laboratory of Structure-Specific Small Molecule Drugs at Chengdu Medical College of Sichuan Province, School of Pharmacy, Chengdu Medical College","place":["Chengdu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Han","sequence":"additional","affiliation":[{"name":"Key Laboratory of Structure-Specific Small Molecule Drugs at Chengdu Medical College of Sichuan Province, 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