{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T06:25:12Z","timestamp":1787034312235,"version":"build-2736575974"},"reference-count":51,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:00:00Z","timestamp":1787011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005206","name":"Chinese Academy of Tropical Agricultural Sciences","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005206","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Insect Sci."],"abstract":"<jats:p>\n                    RNA interference (RNAi) is an eco-friendly strategy for pest management, with double-stranded RNA (dsRNA) as the core functional component. In this study, three RNAi target genes (\n                    <jats:italic>Ubx<\/jats:italic>\n                    ,\n                    <jats:italic>wupA<\/jats:italic>\n                    and\n                    <jats:italic>Dpp<\/jats:italic>\n                    ) with strong lethal effects on\n                    <jats:italic>Apolygus lucorum<\/jats:italic>\n                    were screened via microinjection. The 7-day cumulative mortalities were 56.67 \u00b1 3.33% for ds\n                    <jats:italic>Ubx<\/jats:italic>\n                    , 94.44 \u00b1 1.11% for ds\n                    <jats:italic>wupA<\/jats:italic>\n                    and 92.22 \u00b1 1.11% for ds\n                    <jats:italic>Dpp<\/jats:italic>\n                    . We optimized dsRNA sequences by removing conserved sequences in non-target organisms based on homology alignment and off-target risk analysis. The optimized fragments\n                    <jats:italic>dswupA-OTE<\/jats:italic>\n                    and\n                    <jats:italic>dsDpp-OTE<\/jats:italic>\n                    still exhibited high insecticidal activity, with 7-day cumulative mortalities of 77.78 \u00b1 2.94% and 70.00 \u00b1 1.93%, respectively. We also evaluated the effects of dsRNA length and target sites on RNAi efficiency and screened potent short dsRNA fragments. Novel artificially recombinant dsRNAs were constructed by assembling effective short fragments from different genes, which retained strong insecticidal activity despite shorter sequence length. This study verifies the feasibility of multi-target recombinant dsRNA for pest control and provides a theoretical basis for developing multi-gene RNAi technologies against\n                    <jats:italic>A. lucorum<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.3389\/finsc.2026.1912427","type":"journal-article","created":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:36:29Z","timestamp":1787031389000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening, optimization and artificial recombination of dsRNA fragments for RNAi-mediated pest resistance in Apolygus lucorum"],"prefix":"10.3389","volume":"6","author":[{"given":"Juan","family":"Luo","sequence":"first","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Guo","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]},{"name":"Anhui Province Key Laboratory of Crop Integrated Pest Management\/Key Laboratory of Biology and Sustainable Management of Plant Diseases and Pests of Anhui Higher Education Institutes, College of Plant Protection, Anhui Agricultural University","place":["Hefei, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruyi","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengjun","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guirong","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]},{"name":"Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences","place":["Beijing, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,18]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201301201","article-title":"Fluorescent nanoparticle delivered dsRNA toward genetic control of insect pests","volume":"25","author":"He","year":"2013","journal-title":"Adv Mater"},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4554","article-title":"Evaluation of SmartStax and SmartStax\u2009PRO maize against western corn rootworm and northern corn rootworm: efficacy and resistance management","volume":"73","author":"Head","year":"2017","journal-title":"Pest Manag. 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