{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T18:28:35Z","timestamp":1793039315334,"version":"4.5.0"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,9,29]],"date-time":"2022-09-29T00:00:00Z","timestamp":1664409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000360","name":"Scottish Funding Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000360","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008895","name":"Tertiary Education Trust Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008895","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Trop. Dis"],"abstract":"<jats:p>Novel ideas for control of mosquito-borne disease include the use of bacterial symbionts to reduce transmission. Bacteria belonging to the family Enterobacteriaceae isolated from mosquito midgut have shown promise in limiting <jats:italic>Plasmodium<\/jats:italic> intensity in the <jats:italic>Anopheles<\/jats:italic> vector. However, the mechanism of interaction between bacteria and parasite remains unclear. This study aimed at screening bio-products of two bacteria candidates for their anti-Plasmodial effects on mosquito stages of <jats:italic>P. falciparum. Enterobacter cloacae<\/jats:italic> and <jats:italic>Serratia marcescens<\/jats:italic> were isolated from field-caught <jats:italic>Anopheles gambiae<\/jats:italic> s.l. Spent media from liquid cultures of these bacteria were filtered, lyophilized and dissolved in sterile phosphate buffered saline (PBS). The re-dissolved bacterial products were added to gametocytaemic blood meals and fed to <jats:italic>An. gambiae<\/jats:italic> mosquitoes <jats:italic>via<\/jats:italic> membrane feeders. Control groups were fed on infected blood with or without lyophilized LB medium. The effect of the products on the infection prevalence and intensity of <jats:italic>P. falciparum<\/jats:italic> in mosquitoes was assessed by dissecting mosquito midguts and counting oocysts 10-11 days post-infection. <jats:italic>S. marcescens<\/jats:italic> bio-products elicited significant reduction in the number of mosquitoes infected (<jats:italic>P<\/jats:italic>=4.02 x10<jats:sup>-5<\/jats:sup>) with <jats:italic>P. falciparum<\/jats:italic> and the oocyst intensity (<jats:italic>P<\/jats:italic>&amp;lt;2 x 10<jats:sup>-16<\/jats:sup>) than <jats:italic>E. cloacae<\/jats:italic> products (<jats:italic>P<\/jats:italic>&amp;gt;0.05 for both prevalence and intensity) compared to the control (lyophilized LB medium). These data support the use of bioproducts released by <jats:italic>S. marcescens<\/jats:italic> for malaria control based on transmission blocking in the vector.<\/jats:p>","DOI":"10.3389\/fitd.2022.979615","type":"journal-article","created":{"date-parts":[[2022,9,30]],"date-time":"2022-09-30T12:16:49Z","timestamp":1664540209000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Bio-products from Serratia marcescens isolated from Ghanaian Anopheles gambiae reduce Plasmodium falciparum burden in vector mosquitoes"],"prefix":"10.3389","volume":"3","author":[{"given":"Esinam Abla","family":"Akorli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prince 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