{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T14:20:39Z","timestamp":1782915639668,"version":"3.54.5"},"reference-count":30,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T00:00:00Z","timestamp":1573171200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"DOI":"10.3389\/fmed.2019.00256","type":"journal-article","created":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T07:47:15Z","timestamp":1573199235000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":10,"title":["Identification of Trypanosoma cruzi Polyamine Transport Inhibitors by Computational Drug Repurposing"],"prefix":"10.3389","volume":"6","author":[{"given":"Chantal","family":"Reigada","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melisa","family":"Say\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"IV","given":"Otto","family":"Phanstiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Valera-Vera","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana R.","family":"Miranda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio A.","family":"Pereira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2019,11,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1590\/S0074-02761909000200008","article-title":"Nova Tripanosomiaze humana: estudos sobre amorfolojia e o ciclo evolutivo do Schizotrypanum cruzi n. gen., n. sp., ajente etiolojico de uma nova entidade m\u00f3rbida do homem","volume":"1","author":"Chagas","year":"1909","journal-title":"Mem Inst Oswaldo Cruz"},{"key":"B2","unstructured":"Chagas Disease (American Trypanosomiasis)2019"},{"key":"B3","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/S0140-6736(17)31612-4","article-title":"Chagas disease","volume":"391","author":"Perez-Molina","year":"2018","journal-title":"Lancet"},{"key":"B4","doi-asserted-by":"publisher","first-page":"1295","DOI":"10.1056\/NEJMoa1507574","article-title":"Randomized trial of benznidazole for chronic Chagas' Cardiomyopathy","volume":"373","author":"Morillo","year":"2015","journal-title":"N Engl J Med"},{"key":"B5","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1042\/BJ20110362","article-title":"Polyamine homoeostasis as a drug target in pathogenic protozoa: peculiarities and possibilities","volume":"438","author":"Birkholtz","year":"2011","journal-title":"Biochem J."},{"key":"B6","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1006\/bbrc.2000.2601","article-title":"Polyamines: mysterious modulators of cellular functions","volume":"271","author":"Igarashi","year":"2000","journal-title":"Biochem Biophys Res Commun"},{"key":"B7","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1016\/S0014-5793(99)00804-2","article-title":"Trypanosoma cruzi epimastigotes lack ornithine decarboxylase but can express a foreign gene encoding this enzyme","volume":"454","author":"Carrillo","year":"1999","journal-title":"FEBS Lett."},{"key":"B8","doi-asserted-by":"publisher","first-page":"312","DOI":"10.1111\/j.1550-7408.2003.tb00141.x","article-title":"Lack of arginine decarboxylase in Trypanosoma cruzi epimastigotes","volume":"50","author":"Carrillo","year":"2003","journal-title":"J Eukaryot Microbiol"},{"key":"B9","doi-asserted-by":"publisher","first-page":"936","DOI":"10.1016\/j.bbrc.2006.03.215","article-title":"Molecular and functional characterization of a spermidine transporter (TcPAT12) from Trypanosoma cruzi","volume":"344","author":"Carrillo","year":"2006","journal-title":"Biochem Biophys Res Commun."},{"key":"B10","doi-asserted-by":"publisher","first-page":"78","DOI":"10.1111\/j.1365-2958.2010.07081.x","article-title":"A high-affinity putrescine-cadaverine transporter from Trypanosoma cruzi","volume":"76","author":"Hasne","year":"2010","journal-title":"Mol Microbiol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"e0152715","DOI":"10.1371\/journal.pone.0152715","article-title":"The Trypanosoma cruzi diamine transporter is essential for robust infection of mammalian cells","volume":"11","author":"Hasne","year":"2016","journal-title":"PLoS ONE"},{"key":"B12","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1016\/j.bbrc.2004.07.002","article-title":"Post genomic analysis of permeases from the amino acid\/auxin family in protozoan parasites","volume":"321","author":"Bouvier","year":"2004","journal-title":"Biochem Biophys Res Commun."},{"key":"B13","doi-asserted-by":"publisher","first-page":"36","DOI":"10.3390\/pathogens7020036","article-title":"The uptake and metabolism of amino acids, and their unique role in the biology of pathogenic trypanosomatids","volume":"7","author":"Marchese","year":"2018","journal-title":"Pathogens"},{"key":"B14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.ejmech.2018.01.083","article-title":"Targeting polyamine transport in Trypanosoma cruzi","volume":"147","author":"Reigada","year":"2018","journal-title":"Eur J Med Chem"},{"key":"B15","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1007\/s00232-016-9888-z","article-title":"Trypanosoma cruzi polyamine transporter: its role on parasite growth and survival under stress conditions","volume":"249","author":"Reigada","year":"2016","journal-title":"J Membr Biol"},{"key":"B16","doi-asserted-by":"publisher","first-page":"e0005472","DOI":"10.1371\/journal.pntd.0005472","article-title":"Trypanocidal effect of isotretinoin through the inhibition of polyamine and amino acid transporters in Trypanosoma cruzi","volume":"11","author":"Reigada","year":"2017","journal-title":"PLoS Negl Trop Dis"},{"key":"B17","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1042\/BJ20090815","article-title":"A putrescine-anthracene conjugate: a paradigm for selective drug delivery","volume":"424","author":"Palmer","year":"2009","journal-title":"Biochem J"},{"key":"B18","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1021\/jm401174a","article-title":"Polyamine transport inhibitors: design, synthesis, and combination therapies with difluoromethylornithine","volume":"57","author":"Muth","year":"2014","journal-title":"J Med Chem"},{"key":"B19","doi-asserted-by":"publisher","first-page":"305","DOI":"10.1007\/s00726-007-0527-y","article-title":"Structure-activity investigations of polyamine-anthracene conjugates and their uptake via the polyamine transporter","volume":"33","author":"Phanstiel","year":"2007","journal-title":"Amino Acids"},{"key":"B20","doi-asserted-by":"publisher","first-page":"2874","DOI":"10.1128\/AAC.00106-13","article-title":"Anthracene-polyamine conjugates inhibit in vitro proliferation of intraerythrocytic Plasmodium falciparum parasites","volume":"57","author":"Niemand","year":"2013","journal-title":"Antimicrob Agents Chemother"},{"key":"B21","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1111\/j.1550-7408.1982.tb04024.x","article-title":"Adhesion and interiorization of Trypanosoma cruzi in mammalian cells","volume":"29","author":"Andrews","year":"1982","journal-title":"J Protozool"},{"key":"B22","doi-asserted-by":"publisher","first-page":"566","DOI":"10.1111\/j.1550-7408.1999.tb05132.x","article-title":"L-arginine uptake and L-phosphoarginine synthesis in Trypanosoma cruzi","volume":"46","author":"Pereira","year":"1999","journal-title":"J Eukaryot Microbiol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"e79568","DOI":"10.1371\/journal.pone.0079568","article-title":"SWEETLEAD: an in silico database of approved drugs, regulated chemicals, and herbal isolates for computer-aided drug discovery","volume":"8","author":"Novick","year":"2013","journal-title":"PLoS ONE"},{"key":"B24","doi-asserted-by":"publisher","first-page":"1521","DOI":"10.1021\/acs.jcim.5b00136","article-title":"LiSiCA: a Software for ligand-based virtual screening and its application for the discovery of butyrylcholinesterase inhibitors","volume":"55","author":"Lesnik","year":"2015","journal-title":"J Chem Inf Model"},{"key":"B25","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1021\/ci049885e","article-title":"LigandScout: 3-D pharmacophores derived from protein-bound ligands and their use as virtual screening filters","volume":"45","author":"Wolber","year":"2005","journal-title":"J Chem Inf Model"},{"key":"B26","doi-asserted-by":"publisher","first-page":"2785","DOI":"10.1002\/jcc.21256","article-title":"AutoDock4 and AutoDockTools4: automated docking with selective receptor flexibility","volume":"30","author":"Morris","year":"2009","journal-title":"J Comput Chem"},{"key":"B27","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1038\/319199a0","article-title":"Solvation energy in protein folding and binding","volume":"319","author":"Eisenberg","year":"1986","journal-title":"Nature"},{"key":"B28","doi-asserted-by":"publisher","first-page":"222","DOI":"10.4103\/0975-7406.142943","article-title":"Mini review on tricyclic compounds as an inhibitor of trypanothione reductase","volume":"6","author":"Kumar","year":"2014","journal-title":"J Pharm Bioallied Sci"},{"key":"B29","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/j.exppara.2018.04.016","article-title":"In vitro and in vivo drug combination for the treatment of Trypanosoma cruzi infection: a multivariate approach","volume":"189","author":"Strauss","year":"2018","journal-title":"Exp Parasitol"},{"key":"B30","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1080\/1071576021000046622","article-title":"Phenothiazine radicals inactivate Trypanosoma cruzi dihydrolipoamide dehydrogenase: enzyme protection by radical scavengers","volume":"37","author":"Gutierrez-Correa","year":"2003","journal-title":"Free Radic Res"}],"container-title":["Frontiers in Medicine"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmed.2019.00256\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,8]],"date-time":"2019-11-08T07:47:18Z","timestamp":1573199238000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmed.2019.00256\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,11,8]]},"references-count":30,"alternative-id":["10.3389\/fmed.2019.00256"],"URL":"https:\/\/doi.org\/10.3389\/fmed.2019.00256","relation":{},"ISSN":["2296-858X"],"issn-type":[{"value":"2296-858X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11,8]]},"article-number":"256"}}