{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T15:06:46Z","timestamp":1785337606753,"version":"3.55.0"},"reference-count":54,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T00:00:00Z","timestamp":1581984000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"DOI":"10.3389\/fmed.2020.00043","type":"journal-article","created":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T05:43:58Z","timestamp":1582004638000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":16,"title":["Clinical Outcomes From Cultivated Allogenic Stem Cells vs. Oral Mucosa Epithelial Transplants in Total Bilateral Stem Cells Deficiency"],"prefix":"10.3389","volume":"7","author":[{"given":"Ovidiu","family":"Samoila","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Gocan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2020,2,18]]},"reference":[{"key":"B1","first-page":"516","article-title":"Pathological changes of the anatomical structure and markers of the limbal stem cell niche due to inflammation","volume":"19","author":"Nubile","year":"2013","journal-title":"Mol Vis"},{"key":"B2","volume-title":"Limbal Stem Cells: Identity, Developmental Origin, and Therapeutic Potential","author":"Gonzalez","year":"2018"},{"key":"B3","doi-asserted-by":"publisher","first-page":"593","DOI":"10.2147\/OPTH.S83676","article-title":"Limbal stem cell transplantation: current perspectives","volume":"10","author":"Atallah","year":"2016","journal-title":"Clin Ophthalmol"},{"key":"B4","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1097\/ICO.0000000000000002","article-title":"Cultivation and characterization of limbal epithelial stem cells in rabbits","volume":"55","author":"Samoil\u01ce","year":"2014","journal-title":"Rom J Morphol Embryol"},{"key":"B5","doi-asserted-by":"publisher","first-page":"1946","DOI":"10.1038\/eye.2008.379","article-title":"Limbal stem cell transplantation: new progresses and challenges","volume":"23","author":"Liang","year":"2009","journal-title":"Eye"},{"key":"B6","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1080\/08820538.2017.1353834","article-title":"Limbal stem cell transplantation and complications","volume":"33","author":"Yin","year":"2018","journal-title":"Semin Ophthalmol"},{"key":"B7","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.survophthal.2007.06.013","article-title":"Transplantation of ex vivo cultured limbal epithelial stem cells: a review of techniques and clinical results","volume":"52","author":"Shortt","year":"2007","journal-title":"Surv Ophthalmol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1016\/S0161-6420(89)32833-8","article-title":"Limbal autograft transplantation for ocular surface disorders","volume":"96","author":"Kenyon","year":"1989","journal-title":"Ophthalmology"},{"key":"B9","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1097\/ICO.0000000000000534","article-title":"Management of limbal stem cell deficiency : a historical perspective, past, present, and future","volume":"34","author":"Holland","year":"2015","journal-title":"Cornea"},{"key":"B10","first-page":"489","article-title":"Cultivated limbal stem cell transplantation for ocular surface reconstruction","volume":"2","author":"Burman","year":"2008","journal-title":"Clin Ophthalmol"},{"key":"B11","doi-asserted-by":"publisher","first-page":"990","DOI":"10.1016\/S0140-6736(96)11188-0","article-title":"Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium","volume":"349","author":"Pellegrini","year":"1997","journal-title":"Lancet"},{"key":"B12","first-page":"810","article-title":"Corneal regeneration by induced human buccal mucosa cultivated on an amniotic membrane following alkaline injury","volume":"23","author":"Man","year":"2017","journal-title":"Mol Vis"},{"key":"B13","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1167\/iovs.11-7744","article-title":"Cultured autologous oral mucosal epithelial cell sheet (CAOMECS) transplantation for the treatment of corneal limbal epithelial stem cell deficiency","volume":"53","author":"Burillon","year":"2012","journal-title":"Investig Opthalmology Vis Sci"},{"key":"B14","doi-asserted-by":"publisher","first-page":"181","DOI":"10.4172\/2157-7633.1000181","article-title":"Long-term results of cultured autologous oral mucosa epithelial cell-sheet (CAOMECS) graft for the treatment of blindness due to bilateral limbal stem cell deficiency","volume":"4","author":"Kocaba","year":"2014","journal-title":"J Stem Cell Res Ther"},{"key":"B15","doi-asserted-by":"publisher","first-page":"1253","DOI":"10.1007\/s00417-019-04316-z","article-title":"Comparison of the efficacy of different cell sources for transplantation in total limbal stem cell deficiency","volume":"257","author":"Wang","year":"2019","journal-title":"Graefe's Arch Clin Exp Ophthalmol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1331","DOI":"10.5966\/sctm.2014-0120","article-title":"Spatial distribution of niche and stem cells in ex vivo human limbal cultures","volume":"3","author":"Mariappan","year":"2014","journal-title":"Stem Cells Transl Med"},{"key":"B17","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1007\/s10792-016-0415-0","article-title":"Allogenic cultivated limbal stem cell transplantation versus cadaveric keratolimbal allograft in ocular surface disorder: 1-year outcome","volume":"37","author":"Parihar","year":"2017","journal-title":"Int Ophthalmol"},{"key":"B18","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1016\/j.ophtha.2004.09.023","article-title":"Outcomes and DNA analysis of ex vivo expanded stem cell allograft for ocular surface reconstruction","volume":"112","author":"Daya","year":"2005","journal-title":"Ophthalmology"},{"key":"B19","doi-asserted-by":"publisher","first-page":"945","DOI":"10.1016\/j.ajo.2007.03.005","article-title":"Factors Influencing outcomes in cultivated limbal epithelial transplantation for chronic cicatricial ocular surface disorders","volume":"143","author":"Shimazaki","year":"2007","journal-title":"Am J Ophthalmol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"1337","DOI":"10.1001\/archopht.125.10.1337","article-title":"Phenotypic study after cultivated limbal epithelial transplantation for limbal stem cell deficiency","volume":"125","author":"Kawashima","year":"2007","journal-title":"Arch Ophthalmol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1989","DOI":"10.1016\/j.ophtha.2008.04.039","article-title":"Ex vivo expansion and transplantation of limbal epithelial stem cells","volume":"115","author":"Shortt","year":"2008","journal-title":"Ophthalmology"},{"key":"B22","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1159\/000315020","article-title":"Midterm results of cultivated autologous and allogeneic limbal epithelial transplantation in limbal stem cell deficiency","volume":"45","author":"Pauklin","year":"2010","journal-title":"Res Proj Dry Eye Syndr"},{"key":"B23","doi-asserted-by":"publisher","first-page":"1504","DOI":"10.1136\/bjophthalmol-2012-301869","article-title":"Clinical outcomes of xeno-free allogeneic cultivated limbal epithelial transplantation for bilateral limbal stem cell deficiency","volume":"96","author":"Basu","year":"2012","journal-title":"Br J Ophthalmol"},{"key":"B24","doi-asserted-by":"publisher","first-page":"1483","DOI":"10.2147\/OPTH.S33951","article-title":"Efficacy of cultivated corneal epithelial stem cells for ocular surface reconstruction","volume":"6","author":"Prabhasawat","year":"2012","journal-title":"Clin Ophthalmol"},{"key":"B25","doi-asserted-by":"publisher","first-page":"931","DOI":"10.1016\/j.ophtha.2012.11.001","article-title":"Characteristics of immune rejection after allogeneic cultivated limbal epithelial transplantation","volume":"120","author":"Qi","year":"2013","journal-title":"Ophthalmology"},{"key":"B26","doi-asserted-by":"publisher","first-page":"265","DOI":"10.5966\/sctm.2013-0025","article-title":"Three-year outcomes of cultured limbal epithelial allografts in aniridia and stevens-johnson syndrome evaluated using the clinical outcome assessment in surgical trials assessment tool","volume":"3","author":"Shortt","year":"2014","journal-title":"Stem Cells Transl Med"},{"key":"B27","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1186\/1479-5876-12-58","article-title":"Results of a phase I\/II clinical trial: standardized, non-xenogenic, cultivated limbal stem cell transplantation","volume":"12","author":"Zakaria","year":"2014","journal-title":"J Transl Med."},{"key":"B28","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1155\/2015\/408495","article-title":"Stem cell therapy for corneal epithelium regeneration following good manufacturing and clinical procedures","volume":"2015","author":"Ram\u00edrez","year":"2015","journal-title":"Biomed Res Int"},{"key":"B29","doi-asserted-by":"publisher","first-page":"480983","DOI":"10.1155\/2015\/480983","article-title":"Long-term outcomes of cultivated limbal epithelial transplantation: evaluation and comparison of results in children and adults","volume":"2015","author":"Ganger","year":"2015","journal-title":"Biomed Res Int"},{"key":"B30","doi-asserted-by":"publisher","first-page":"1765","DOI":"10.1007\/s00417-016-3410-2","article-title":"Characterization of the corneal surface in limbal stem cell deficiency and after transplantation of cultured allogeneic limbal epithelial cells","volume":"254","author":"Chen","year":"2016","journal-title":"Graefe's Arch Clin Exp Ophthalmol"},{"key":"B31","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1186\/s12886-017-0403-9","article-title":"Long-term outcome of allogeneic cultivated limbal epithelial transplantation for symblepharon caused by severe ocular burns","volume":"17","author":"Cheng","year":"2017","journal-title":"BMC Ophthalmol"},{"key":"B32","doi-asserted-by":"publisher","first-page":"1543","DOI":"10.1097\/ICO.0000000000002153","article-title":"Short- and long-term results of xenogeneic-free cultivated autologous and allogeneic limbal epithelial stem cell transplantations","volume":"38","author":"Behaegel","year":"2019","journal-title":"Cornea."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1230","DOI":"10.1002\/sctm.19-0021","article-title":"Long-term results of cultured limbal stem cell versus limbal tissue transplantation in stage iii limbal deficiency","volume":"8","author":"Borderie","year":"2019","journal-title":"Stem Cells Transl Med"},{"key":"B34","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1002\/sctm.18-0140","article-title":"Allogeneic ex vivo expanded corneal epithelial stem cell transplantation: a randomized controlled clinical trial","volume":"8","author":"Campbell","year":"2019","journal-title":"Stem Cells Transl Med"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.1056\/NEJMoa040455","article-title":"Corneal reconstruction with tissue-engineered cell sheets composed of autologous oral mucosal epithelium","volume":"351","author":"Nishida","year":"2004","journal-title":"N Engl J Med."},{"key":"B36","doi-asserted-by":"publisher","first-page":"4660","DOI":"10.1167\/iovs.09-3377","article-title":"Persistence of transplanted oral mucosal epithelial cells in human cornea","volume":"50","author":"Chen","year":"2009","journal-title":"Investig Opthalmol Vis Sci"},{"key":"B37","doi-asserted-by":"publisher","first-page":"942","DOI":"10.1136\/bjo.2010.188714","article-title":"Long-term results of autologous cultivated oral mucosal epithelial transplantation in the scar phase of severe ocular surface disorders","volume":"95","author":"Nakamura","year":"2011","journal-title":"Br J Ophthalmol"},{"key":"B38","doi-asserted-by":"publisher","first-page":"1524","DOI":"10.1016\/j.ophtha.2011.01.039","article-title":"Long-term Outcome of cultivated oral mucosal epithelial sheet transplantation in treatment of total limbal stem cell deficiency","volume":"118","author":"Satake","year":"2011","journal-title":"Ophthalmology"},{"key":"B39","doi-asserted-by":"publisher","first-page":"1641","DOI":"10.1038\/eye.2011.230","article-title":"Adult human buccal epithelial stem cells: Identification, ex-vivo expansion, and transplantation for corneal surface reconstruction","volume":"25","author":"Priya","year":"2011","journal-title":"Eye."},{"key":"B40","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/j.ophtha.2012.07.053","article-title":"Visual Improvement after cultivated oral mucosal epithelial transplantation","volume":"120","author":"Sotozono","year":"2013","journal-title":"Ophthalmology."},{"key":"B41","doi-asserted-by":"publisher","first-page":"2135","DOI":"10.1002\/stem.1694","article-title":"Successful application of ex vivo expanded human autologous oral mucosal epithelium for the treatment of total bilateral limbal stem cell deficiency","volume":"32","author":"Kolli","year":"2014","journal-title":"Stem Cells"},{"key":"B42","doi-asserted-by":"publisher","first-page":"281870","DOI":"10.1155\/2015\/281870","article-title":"Cultivated oral mucosa epithelium in ocular surface reconstruction in aniridia patients","volume":"2015","author":"Dobrowolski","year":"2015","journal-title":"Biomed Res Int."},{"key":"B43","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1097\/ICO.0000000000001409","article-title":"Prospective clinical trial of corneal reconstruction with sheets","volume":"37","author":"Kim","year":"2017","journal-title":"Cornea"},{"key":"B44","doi-asserted-by":"publisher","first-page":"345","DOI":"10.1089\/ten.teb.2014.0547","article-title":"Feeder layer cell actions and applications","volume":"21","author":"Llames","year":"2015","journal-title":"Tissue Eng Part B Rev"},{"key":"B45","doi-asserted-by":"publisher","first-page":"806","DOI":"10.4252\/wjsc.v7.i5.806","article-title":"Corneal stem cells and tissue engineering: current advances and future perspectives","volume":"7","author":"de Araujo","year":"2015","journal-title":"World J Stem Cells"},{"key":"B46","doi-asserted-by":"publisher","first-page":"139","DOI":"10.15252\/emmm.201708213","article-title":"Regenerating human epithelia with cultured stem cells: feeder cells, organoids and beyond","volume":"10","author":"Hynds","year":"2018","journal-title":"EMBO Mol Med"},{"key":"B47","doi-asserted-by":"publisher","first-page":"76","DOI":"10.1097\/ICO.0000000000001409","article-title":"Prospective clinical trial of corneal reconstruction with biomaterial-free cultured oral mucosal epithelial cell sheets","volume":"37","author":"Kim","year":"2018","journal-title":"Cornea"},{"key":"B48","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1167\/iovs.06-0514","article-title":"The fate of limbal epithelial progenitor cells during explant culture on intact amniotic membrane","volume":"48","author":"Li","year":"2007","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"B49","doi-asserted-by":"publisher","first-page":"2685","DOI":"10.1007\/s10792-019-01092-x","article-title":"Systematic review and meta-analysis investigating autograft versus allograft cultivated limbal epithelial transplantation in limbal stem cell deficiency","volume":"39","author":"Mishan","year":"2019","journal-title":"Int Ophthalmol."},{"key":"B50","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.clae.2019.04.006","article-title":"Recovering vision in corneal epithelial stem cell deficient eyes","volume":"42","author":"Bains","year":"2019","journal-title":"Contact Lens Anterior Eye"},{"key":"B51","doi-asserted-by":"publisher","first-page":"1160","DOI":"10.5966\/sctm.2014-0064","article-title":"Concise review: the coming of age of stem cell treatment for corneal surface damage","volume":"3","author":"Ramachandran","year":"2014","journal-title":"Stem Cells Transl Med."},{"key":"B52","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.trsl.2018.11.003","article-title":"A proof-of-concept clinical trial using mesenchymal stem cells for the treatment of corneal epithelial stem cell deficiency","volume":"206","author":"Calonge","year":"2019","journal-title":"Transl Res"},{"key":"B53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1186\/s13287-018-1036-5","article-title":"Corneal cell therapy: with iPSCs, it is no more a far-sight","volume":"9","author":"Chakrabarty","year":"2018","journal-title":"Stem Cell Res Ther."},{"key":"B54","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1038\/d41586-019-02597-2","article-title":"Woman is first to receive cornea made from reprogrammed stem cells","volume":"573","author":"Cyranoski","year":"2019","journal-title":"Nature"}],"container-title":["Frontiers in Medicine"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmed.2020.00043\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,18]],"date-time":"2020-02-18T05:44:00Z","timestamp":1582004640000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmed.2020.00043\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,2,18]]},"references-count":54,"alternative-id":["10.3389\/fmed.2020.00043"],"URL":"https:\/\/doi.org\/10.3389\/fmed.2020.00043","relation":{},"ISSN":["2296-858X"],"issn-type":[{"value":"2296-858X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,18]]},"article-number":"43"}}