{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T13:45:37Z","timestamp":1784641537966,"version":"3.55.0"},"reference-count":41,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T00:00:00Z","timestamp":1632355200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p>mNGS(metagenomics Next Generation Sequencing), as a novel culture-independent approach, demonstrated the capability of rapid, sensitive, and accurate pathogen identification. At present, there have been many case reports about the use of mNGS to assist in the diagnosis of bacterial, fungal, viral and parasitic infections and to guide clinicians to determine appropriate treatment. However, the clinical understanding of this technique is not comprehensive, and the experience of using it is relatively limited. We reported a 53-year-old man who was admitted to hospital with a high fever and headache. His inflammatory biomarkers were markedly elevated. Based on the clinical presentation, He was initially diagnosed as having an intracranial infection of unknown etiology and received empirical antibiotics and systemic supportive treatment. But these did not relieve his symptoms. Both the blood and CSF specimens were examined using traditional culture, serological testing, and mNGS. Traditional culture and serological testing produced negative results, while the mNGS revealed the presence of a potential pathogen, <jats:italic>mucor<\/jats:italic>, in the CSF specimen. Then targeted antifungal treatment was selected quickly and his temperature gradually returned to normal. Thus, we report the case in which mNGS was an auxiliary method to diagnose <jats:italic>mucormycosis<\/jats:italic>, and discuss this case in combination with relevant literature, in order to improve the clinical cognition of this technology.<\/jats:p>","DOI":"10.3389\/fmed.2021.682758","type":"journal-article","created":{"date-parts":[[2021,9,23]],"date-time":"2021-09-23T05:26:26Z","timestamp":1632374786000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":18,"title":["Case Report: Diagnostic Value of Metagenomics Next Generation Sequencing in Intracranial Infection Caused by Mucor"],"prefix":"10.3389","volume":"8","author":[{"given":"YuChen","family":"Liu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Han","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"LiJuan","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ZhaoYang","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"ChunCheng","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YongGang","family":"Luo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2021,9,23]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1111\/1469-0691.12371","article-title":"ESCMID and ECMM joint clinical guidelines for the diagnosis and management of mucormycosis 2013","volume":"20","author":"Cornely","year":"2014","journal-title":"Clin Microbiol Infect."},{"key":"B2","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1086\/432579","article-title":"Epidemiology and outcome of zygomycosis: a review of 929 reported cases","volume":"41","author":"Roden","year":"2005","journal-title":"Clin Infect Dis."},{"key":"B3","doi-asserted-by":"publisher","first-page":"686","DOI":"10.3760\/j.issn:0578-1426.2004.09.015","article-title":"The clinical and pathological features of rhinocerebral mucormycosis","volume":"43","author":"Hong","year":"2004","journal-title":"Chin J Internal Med."},{"key":"B4","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1097\/00000637-199909000-00016","article-title":"Rhinocerebral mucormycosis: report of two cases","volume":"43","author":"Akoz","year":"1999","journal-title":"Ann Plast Surg."},{"key":"B5","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0039-6257(05)80041-4","article-title":"Survival factors in rhino-orbital-cerebral mucormycosis","volume":"39","author":"Yohai","year":"1994","journal-title":"Surv Ophthalmol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1097\/MAJ.0b013e3181f0aad1","article-title":"Rhinocerebral mucormycosis complicated by bacterial brain abscess","volume":"340","author":"Cano","year":"2010","journal-title":"Am J Med Sci."},{"key":"B7","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/S2213-8587(15)00522-7","article-title":"Rhino-orbital mucormycosis in a patient with diabetes","volume":"5","author":"Bakshi","year":"2017","journal-title":"Lancet Diabetes Endocrinol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"1054","DOI":"10.3892\/etm.2017.4074","article-title":"Rapidly progressive rhino-orbito-cerebral mucormycosis in a patient with type 2 diabetes: a case report","volume":"13","author":"Chen","year":"2017","journal-title":"Exp Ther Med."},{"key":"B9","doi-asserted-by":"publisher","first-page":"503","DOI":"10.1086\/590004","article-title":"Delaying amphotericin B-based frontline therapy significantly increases mortality among patients with hematologic malignancy who have zygomycosis","volume":"47","author":"Chamilos","year":"2008","journal-title":"Clin Infect Dis."},{"key":"B10","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1288\/00005537-199206000-00011","article-title":"Griswold JA. 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