{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:37:40Z","timestamp":1780447060376,"version":"3.54.1"},"reference-count":28,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T00:00:00Z","timestamp":1638403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010270","name":"Capital Health Research and Development of Special Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100010270","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p><jats:bold>Purpose:<\/jats:bold> To evaluate the feasibility of <jats:italic>in-vivo<\/jats:italic> imaging of the retina and choroid using spectral domain optical coherence tomography (OCT) in guinea pigs.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> The study included 19 pigmented guinea pigs (age: 3\u20134 weeks) which underwent sonographic axial length measurements and OCT imaging. At study end, the animals were sacrificed and histomorphometric examinations of the retina and choroid were performed. We assessed the reproducibility of the OCT measurements and compared <jats:italic>in-vivo<\/jats:italic> measurements to histomorphometric data.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> The mean thickness of the retina and choroid near the optic nerve head was 175.6 \u00b1 25.8 and 63.4 \u00b1 16.5 \u03bcm, respectively, and mean Bruch's membrane opening (BMO) diameter was 831 \u00b1 121 \u03bcm. The intra-observer comparison of measurements of retinal thickness (intraclass correlation coefficient (ICC) = 0.92, 95% CI: 0.86\u20130.96; <jats:italic>P<\/jats:italic> &amp;lt; 0.001), choroidal thickness (ICC = 0.92, 95% CI: 0.86\u20130.96; <jats:italic>P<\/jats:italic> &amp;lt; 0.001), and BMO diameter (ICC = 0.92, 95% CI: 0.86\u20130.96; <jats:italic>P<\/jats:italic> &amp;lt; 0.001) showed a high correlation. A high agreement was present also for the inter-observer reproducibility of the measurements of retinal thickness (Pearson correlation coefficient (<jats:italic>R<\/jats:italic>) = 0.98; <jats:italic>P<\/jats:italic> &amp;lt; 0.001), choroidal thickness (<jats:italic>R<\/jats:italic> = 0.96; <jats:italic>P<\/jats:italic> &amp;lt; 0.001), and BMO diameter (<jats:italic>R<\/jats:italic> = 0.98; <jats:italic>P<\/jats:italic> &amp;lt; 0.001). The Bland-Altman plots showed that 2.6% (1\/38), 5.3% (2\/38), and 7.9% (3\/38) of the measurement points of retinal thickness, choroidal thickness and BMO diameter, respectively, were located outside of the 95% limits of agreement. The OCT-based thickness measurements of retina and choroid were significantly higher than those measured by histomorphometry (both <jats:italic>P<\/jats:italic>-values &amp;lt;0.01).<\/jats:p><jats:p><jats:bold>Conclusion:<\/jats:bold> OCT-based <jats:italic>in-vivo<\/jats:italic> morphometric imaging of the retina and choroid in guinea pigs is feasible with an acceptable intra-observer repeatability and inter-observer reproducibility.<\/jats:p>","DOI":"10.3389\/fmed.2021.730494","type":"journal-article","created":{"date-parts":[[2021,12,2]],"date-time":"2021-12-02T09:16:21Z","timestamp":1638436581000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":7,"title":["In vivo Imaging of Retina and Choroid in Guinea Pigs"],"prefix":"10.3389","volume":"8","author":[{"given":"Li","family":"Dong","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi 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