{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T11:58:50Z","timestamp":1788350330143,"version":"build-2803163510"},"reference-count":39,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T00:00:00Z","timestamp":1642032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p><jats:bold>Background:<\/jats:bold> Breast cancer is the most common malignancy and a leading cause of death among women. The majority of patients require surgery, and retrospective studies have revealed an association between anaesthetic techniques during surgery and clinical outcomes. Local anaesthetics (LAs) influence carcinogenesis by interacting with non-coding RNAs (ncRNAs). However, the detailed mechanisms underlying the association between LAs and ncRNAs remain unclear.<\/jats:p><jats:p><jats:bold>Methods:<\/jats:bold> In this study, the effects of two commonly used LAs, lidocaine and bupivacaine, on the malignancy of MCF-7 breast cancer cells were investigated. The expression profiles of the microRNAs (miRNAs) that responded to treatment with LAs were determined through next-generation sequencing.<\/jats:p><jats:p><jats:bold>Results:<\/jats:bold> Data from the functional assay revealed that the LAs suppressed the proliferation of MCF-7 cells. The result of next-generation sequencing revealed that 131 miRNAs were upregulated, following treatment with the LAs. Validation using polymerase chain reaction (PCR) identified <jats:italic>miR-187-5p<\/jats:italic> as a potential biomarker, and it was selected for further analyses. Prediction with bioinformatics tools and luciferase reporter assays revealed that <jats:italic>MYB<\/jats:italic> is a direct target gene of <jats:italic>miR-187-5p<\/jats:italic>. Based on the hypothesis that lncRNAs acts as miRNA sponges, the target lncRNA, <jats:italic>DANCR<\/jats:italic>, of <jats:italic>miR-187-5p<\/jats:italic> was predicted using DIANA-LncBase v2 and validated using luciferase reporter assays. In addition, the reciprocal suppressive effect between <jats:italic>DANCR<\/jats:italic> and <jats:italic>miR-187-5p<\/jats:italic> was determined.<\/jats:p><jats:p><jats:bold>Conclusions:<\/jats:bold> This study suggests that one of the anti-tumour mechanisms of lidocaine and bupivacaine is mediated through the <jats:italic>DANCR-miR-187-5p-MYB<\/jats:italic> axis. This may provide a novel molecular mechanism of tumour suppression in breast cancer.<\/jats:p>","DOI":"10.3389\/fmed.2021.732817","type":"journal-article","created":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T05:13:39Z","timestamp":1642050819000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":5,"title":["Lidocaine and Bupivacaine Downregulate MYB and DANCR lncRNA by Upregulating miR-187-5p in MCF-7 Cells"],"prefix":"10.3389","volume":"8","author":[{"given":"Chiao-Yi","family":"Lin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Ting","family":"Tseng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao-Yin","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mong-Hsun","family":"Tsai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric Y.","family":"Chuang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu-Pin","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Chuan","family":"Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,1,13]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1807","DOI":"10.1016\/S0140-6736(19)32313-X","article-title":"Recurrence of breast cancer after regional or general anaesthesia: a randomised controlled trial","volume":"394","author":"Sessler","year":"2019","journal-title":"Lancet."},{"key":"B2","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.bja.2019.04.062","article-title":"Influence of perioperative anaesthetic and analgesic interventions on oncological outcomes: a narrative review","volume":"123","author":"Wall","year":"2019","journal-title":"Br J Anaesth."},{"key":"B3","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1097\/ACO.0000000000000360","article-title":"Opioids and tumour metastasis: does the choice of the anesthetic-analgesic technique influence outcome after cancer surgery?","volume":"29","author":"Connolly","year":"2016","journal-title":"Curr Opin Anaesthesiol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1111\/anae.15243","article-title":"The role of regional anaesthesia in the emerging subspecialty of onco-anaesthesia: a state-of-the-art review","volume":"76","author":"Dockrell","year":"2021","journal-title":"Anaesthesia."},{"key":"B5","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1186\/s12885-018-4576-2","article-title":"Effects of local anesthetics on breast cancer cell viability and migration","volume":"18","author":"Li","year":"2018","journal-title":"BMC Cancer."},{"key":"B6","doi-asserted-by":"publisher","first-page":"26277","DOI":"10.1038\/srep26277","article-title":"Local anesthetic bupivacaine induced ovarian and prostate cancer apoptotic cell death and underlying mechanisms in vitro","volume":"6","author":"Xuan","year":"2016","journal-title":"Sci Rep."},{"key":"B7","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.bpa.2019.03.003","article-title":"Breast surgery and regional anaesthesia","volume":"33","author":"FitzGerald","year":"2019","journal-title":"Best Pract Res Clin Anaesthesiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"962","DOI":"10.1016\/j.bja.2018.07.015","article-title":"Lidocaine inhibits cytoskeletal remodelling and human breast cancer cell migration","volume":"121","author":"D'Agostino","year":"2018","journal-title":"Br J Anaesth."},{"key":"B9","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1097\/ALN.0b013e31824babfe","article-title":"Antiproliferative effects of local anesthetics on mesenchymal stem cells: potential implications for tumor spreading and wound healing","volume":"116","author":"Lucchinetti","year":"2012","journal-title":"Anesthesiology."},{"key":"B10","doi-asserted-by":"publisher","first-page":"235","DOI":"10.3389\/fmed.2017.00235","article-title":"The amide local anesthetic lidocaine in cancer surgery-potential antimetastatic effects and preservation of immune cell function? 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