{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:00:09Z","timestamp":1753891209742,"version":"3.41.2"},"reference-count":32,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T00:00:00Z","timestamp":1643155200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82002186","82073611"],"award-info":[{"award-number":["82002186","82073611"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p><jats:italic>Acinetobacter baumannii<\/jats:italic> is an important, opportunistic nosocomial pathogen that causes a variety of nosocomial infections, and whose drug resistance rate has increased in recent years. The CRISPR-Cas system exists in several bacteria, providing adaptive immunity to foreign nucleic acid invasion. This study explores whether CRISPR-Cas is related to drug resistance. Antibiotics were used to treat strains ATCC19606 and AB43, and the expression of CRISPR-related genes was found to be changed. The Csy proteins (Csy1\u20134) were previously detected to promote target recognition; however, the potential function of <jats:italic>csy1<\/jats:italic> gene is still unknown. Thus, the Rec<jats:sub>Ab<\/jats:sub> homologous recombination system was utilized to knock out the <jats:italic>csy1<\/jats:italic> gene from <jats:italic>A. baumannii<\/jats:italic> AB43, which carries the Type I-Fb CRISPR-Cas system, and to observe the drug resistance changes in wild-type and <jats:italic>csy1<\/jats:italic>-deleted strains. The AB43\u0394<jats:italic>csy1<\/jats:italic> mutant strain was found to become resistant to antibiotics, while the wild-type strain was sensitive to antibiotics. Moreover, transcriptome analysis revealed that the <jats:italic>csy1<\/jats:italic> gene regulates genes encoding CRISPR-Cas-related proteins, drug-resistant efflux pumps, membrane proteins, and oxidative phosphorylation-related proteins, inhibiting antimicrobial resistance in <jats:italic>A. baumannii<\/jats:italic>. The <jats:italic>in vitro<\/jats:italic> resistance development assay revealed that the complete CRISPR-Cas system could inhibit the development of bacterial resistance. Our findings expand our understanding of the role of CRISPR-Cas <jats:italic>csy1<\/jats:italic> gene in <jats:italic>A. baumannii<\/jats:italic> and link the CRISPR-Cas system to the biogenesis of bacterial drug-resistant structures.<\/jats:p>","DOI":"10.3389\/fmed.2022.797104","type":"journal-article","created":{"date-parts":[[2022,1,26]],"date-time":"2022-01-26T05:22:40Z","timestamp":1643174560000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":11,"title":["The Involvement of the csy1 Gene in the Antimicrobial Resistance of Acinetobacter 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