{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T14:36:43Z","timestamp":1783953403218,"version":"3.55.0"},"reference-count":37,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T00:00:00Z","timestamp":1654732800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p>This study has focused on determining the association of m6A methyltransferase [methyltransferase-like 3 (METTL3), methyltransferase-like 14 (METTL14), and Wilms tumor 1-associating protein (WTAP)], demethylase [fat mass and obesity-associated protein (FTO) and alkylation repair homolog protein 5 (ALKBH5)], RNA-binding proteins [YT521-B homology domains 2 (YTHDF2)], and ankylosing spondylitis (AS). A total of 154 specimens, containing 79 patients with new-onset AS and 75 healthy controls (HCs), participated in the study. The mRNA expressions of these m6A methyltransferase, demethylase, and RNA-binding protein in peripheral blood mononuclear cells (PBMCs) were detected by quantitative real-time PCR (qRT-PCR). The data showed that the mRNA expressions of <jats:italic>YTHDF2<\/jats:italic> and <jats:italic>ALKBH5<\/jats:italic> in PBMC from patients with new-onset AS were significantly decreased, and there was a positive correlation between RNA-binding proteins (<jats:italic>YTHDF2<\/jats:italic>) and demethylase (<jats:italic>ALKBH5<\/jats:italic>) in patients with new-onset AS. Logistic regression analysis demonstrated that the expression of <jats:italic>YTHDF2<\/jats:italic> mRNA in PBMC is a risk factor of AS. Receiver operating characteristic (ROC) analysis of the area under the curve (AUC) for mRNA <jats:italic>YTHDF2<\/jats:italic> in new-onset AS and HC was 0.692, with a cutoff value of &amp;lt;0.8724, a sensitivity of 67%, and a specificity of 63%. Moreover, we constructed a novel predictive model based on a combination of mRNA <jats:italic>YTHDF2<\/jats:italic> and systemic immune-inflammation index (SII) for AS diagnosis (AUC = 0.865, sensitivity = 79.45%, specificity = 84.00%), and the predictive model correlated with the activity and severity of AS. This study indicates that the mRNA expression of <jats:italic>YTHDF2<\/jats:italic> in PBMC may be involved in AS pathogenesis and a predictive model based on a combination of mRNA <jats:italic>YTHDF2<\/jats:italic> and SII acts as a marker for diagnosis and progression of diseases.<\/jats:p>","DOI":"10.3389\/fmed.2022.922219","type":"journal-article","created":{"date-parts":[[2022,6,9]],"date-time":"2022-06-09T06:25:29Z","timestamp":1654755929000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Expression and Clinical Significance of the m6A RNA-Binding Proteins YTHDF2 in Peripheral Blood Mononuclear Cells From New-Onset Ankylosing 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