{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T22:07:45Z","timestamp":1792102065583,"version":"4.4.0"},"reference-count":27,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:00:00Z","timestamp":1684454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p>Clinical case of a patient with a <jats:italic>Pseudomonas aeruginosa<\/jats:italic> multidrug-resistant prosthetic vascular graft infection which was treated with a cocktail of phages (PT07, 14\/01, and PNM) in combination with ceftazidime-avibactam (CZA). After the application of the phage treatment and in absence of antimicrobial therapy, a new <jats:italic>P. aeruginosa<\/jats:italic> bloodstream infection (BSI) with a septic residual limb metastasis occurred, now involving a wild-type strain being susceptible to \u00df-lactams and quinolones. Clinical strains were analyzed by microbiology and whole genome sequencing techniques. In relation with phage administration, the clinical isolates of <jats:italic>P. aeruginosa<\/jats:italic> before phage therapy (HE2011471) and post phage therapy (HE2105886) showed a clonal relationship but with important genomic changes which could be involved in the resistance to this therapy. Finally, phenotypic studies showed a decrease in Minimum Inhibitory Concentration (MIC) to \u00df-lactams and quinolones as well as an increase of the biofilm production and phage resistant mutants in the clinical isolate of <jats:italic>P. aeruginosa<\/jats:italic> post phage therapy.<\/jats:p>","DOI":"10.3389\/fmed.2023.1199657","type":"journal-article","created":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T05:58:17Z","timestamp":1684475897000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":62,"title":["Case report: Analysis of phage therapy failure in a patient with a Pseudomonas aeruginosa prosthetic vascular graft infection"],"prefix":"10.3389","volume":"10","author":[{"given":"Lucia","family":"Blasco","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada","family":"L\u00f3pez-Hern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Rodr\u00edguez-Fern\u00e1ndez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"P\u00e9rez-Florido","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos S.","family":"Casimiro-Soriguer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Djebara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Merabishvili","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Paul","family":"Pirnay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jes\u00fas","family":"Rodr\u00edguez-Ba\u00f1o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda","family":"Tom\u00e1s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis Eduardo","family":"L\u00f3pez Cort\u00e9s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,5,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1111\/j.1469-0691.2011.03618.x","article-title":"Characteristics and prognosis in patients with prosthetic vascular graft infection: a prospective observational cohort study","volume":"18","author":"Legout","year":"2012","journal-title":"Clin Microbiol Infect."},{"key":"B2","article-title":"Infections and Antibiotics in Vascular Surgery","volume-title":"Basic Science and Clinical Correlations","author":"SR","year":"1994"},{"key":"B3","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/S1473-3099(17)30753-3","article-title":"Discovery, research, and development of new antibiotics: the WHO priority list of antibiotic-resistant bacteria and tuberculosis","volume":"18","author":"Tacconelli","year":"2018","journal-title":"Lancet Infect Dis."},{"key":"B4","doi-asserted-by":"publisher","first-page":"1906","DOI":"10.1128\/AAC.01645-10","article-title":"Spanish Network for Research in Infectious Diseases (REIPI). 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