{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T05:28:11Z","timestamp":1780464491823,"version":"3.54.1"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T00:00:00Z","timestamp":1704672000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Assessment of kidney function in emergency settings is essential across all medical subspecialties. Daily assessment of patient creatinine results from emergency medical services showed that some deviated from expected values, implying drug-related interference.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Real-time clinical evaluation of an enzyme method (Roche CREP2) in comparison with the Jaff\u00e9 gen. 2 method (Roche CREJ2) was performed. During the period of December 2022 and January 2023, we analyzed 8,498 patient samples, where 5,524 were heavily medicated STAT patient specimens, 500 were pediatric specimens, and 2,474 were from a distant general population in a different region using the same methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In 109 out of 5,524 hospital specimens (1.97%, <jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.001), the CREP2 value was apparently (25% or more) lower than CREJ2. Suspect interfering medication was found in a sample of 43 out of 46 reviewed patients where medication data were available. This phenomenon was not observed in the general population.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In a polymedicated urgent care hospital population, a creatinine enzyme method produces unreliable results, apparently due to multiple drug-related interferences.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmed.2023.1236948","type":"journal-article","created":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T06:32:12Z","timestamp":1704695532000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":2,"title":["Emergency medicine pharmacotherapy compromises accuracy of plasma creatinine determination by enzyme-based methods: real-world clinical evidence and implications for clinical practice"],"prefix":"10.3389","volume":"10","author":[{"given":"Regina","family":"Demlova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarka","family":"Kozakova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Rihacek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Buckova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Horska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ondrej","family":"Wiewiorka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lubos","family":"Boucek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iveta","family":"Selingerova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martina","family":"Podborska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Korberova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Mikuskova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiri","family":"Starha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miroslava","family":"Benovska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Radina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Richter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenka","family":"Zdrazilova Dubska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dalibor","family":"Valik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2024,1,8]]},"reference":[{"key":"ref1","first-page":"391","article-title":"Ueber den Niederschlag, welchen Pikrins\u00e4ure in normalem Harn erzeugt und \u00fcber eine neue Reaction des Kreatinins","volume":"10","author":"Jaffe","year":"1886","journal-title":"Z Physiol Chem"},{"key":"ref2","first-page":"889","article-title":"Multicentre evaluation of an enzymatic method for creatinine determination using a sensitive colour reagent","volume":"24","author":"Guder","year":"1986","journal-title":"J Clin Chem Clin Biochem"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"449","DOI":"10.3109\/00365513.2013.794300","article-title":"Strong negative interference of ethamsylate (Dicynone\u00ae) in serum creatinine quantification via enzymatic assay using Trinder reaction","volume":"73","author":"Wiewiorka","year":"2013","journal-title":"Scand J Clin Lab Invest"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"474","DOI":"10.1177\/00045632211020029","article-title":"Drug dosing using estimated glomerular filtration rate: misclassification due to metamizole interference in a creatinine assay","volume":"58","author":"Bojko","year":"2021","journal-title":"Ann Clin Biochem"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1016\/j.clinbiochem.2015.01.007","article-title":"In vitro interference by acetaminophen, aspirin, and metamizole in serum measurements of glucose, urea, and creatinine","volume":"48","author":"Luna-Z\u00e1izar","year":"2015","journal-title":"Clin Biochem"},{"key":"ref6","doi-asserted-by":"publisher","first-page":"1973","DOI":"10.1016\/j.ekir.2021.04.006","article-title":"N-acetylcysteine interference with creatinine measurement: an in vitro analysis","volume":"6","author":"McCudden","year":"2021","journal-title":"Kidney Int Rep"},{"key":"ref7","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.cca.2021.12.001","article-title":"Plasma creatinine below limit of quantification in a patient with acute kidney injury","volume":"524","author":"Orieux","year":"2022","journal-title":"Clin Chim Acta"},{"key":"ref8","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.cca.2006.05.012","article-title":"Mechanism of ascorbic acid interference in biochemical tests that use peroxide and peroxidase to generate chromophore","volume":"373","author":"Martinello","year":"2006","journal-title":"Clin Chim Acta"},{"key":"ref9","doi-asserted-by":"publisher","first-page":"e23928","DOI":"10.1002\/jcla.23928","article-title":"A new method for anti-negative interference of calcium dobesilate in serum creatinine enzymatic analysis","volume":"35","author":"Shen","year":"2021","journal-title":"J Clin Lab Anal"},{"key":"ref10","doi-asserted-by":"publisher","first-page":"1732","DOI":"10.1373\/clinchem.2009.127373","article-title":"Catecholamine interference in enzymatic creatinine assays","volume":"55","author":"Saenger","year":"2009","journal-title":"Clin Chem"},{"key":"ref11","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1177\/00045632221074867","article-title":"Negative interference from immunoglobulin M paraproteinaemia on the Roche enzymatic creatinine method","volume":"59","author":"Flowers","year":"2022","journal-title":"Ann Clin Biochem"},{"key":"ref12","doi-asserted-by":"publisher","first-page":"bcr2016217946","DOI":"10.1136\/bcr-2016-217946","article-title":"Deceiving proteins! A case of lymphoma and high creatinine","volume":"2017","author":"Metraiah","year":"2017","journal-title":"BMJ Case Rep"},{"key":"ref13","year":"2022"},{"key":"ref14","year":"2022"},{"key":"ref15","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","volume-title":"ggplot2: Elegant graphics for data analysis","author":"Wickham","year":"2016"},{"key":"ref16","author":"Rudis","year":"2020"},{"key":"ref17","doi-asserted-by":"publisher","first-page":"416059","DOI":"10.1155\/2015\/416059","article-title":"Serum cystatin C for the diagnosis of acute kidney injury in patients admitted in the emergency department","volume":"2015","author":"Bongiovanni","year":"2015","journal-title":"Dis Markers"},{"key":"ref18","doi-asserted-by":"publisher","first-page":"1737","DOI":"10.1056\/NEJMoa2102953","article-title":"New creatinine-and cystatin C-based equations to estimate GFR without race","volume":"385","author":"Inker","year":"2021","journal-title":"N Engl J Med"},{"key":"ref19","year":""},{"key":"ref20","year":""},{"key":"ref21","year":""},{"key":"ref22","year":"2022"},{"key":"ref23","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1681\/ASN.2008030287","article-title":"New equations to estimate GFR in children with CKD","volume":"20","author":"Schwartz","year":"2009","journal-title":"J Am Soc Nephrol"},{"key":"ref24","doi-asserted-by":"publisher","first-page":"c179","DOI":"10.1159\/000339789","article-title":"KDIGO clinical practice guidelines for acute kidney injury","volume":"120","author":"Khwaja","year":"2012","journal-title":"Nephron Clin Pract"},{"key":"ref25","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1038\/s41572-021-00284-z","article-title":"Acute kidney injury","volume":"7","author":"Kellum","year":"2021","journal-title":"Nat Rev Dis Primers"},{"key":"ref26","doi-asserted-by":"publisher","first-page":"103692","DOI":"10.1016\/j.bioorg.2020.103692","article-title":"Mechanisms of interference of p-diphenols with the Trinder reaction","volume":"97","author":"Tarasek","year":"2020","journal-title":"Bioorg Chem"}],"container-title":["Frontiers in Medicine"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmed.2023.1236948\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,8]],"date-time":"2024-01-08T06:32:15Z","timestamp":1704695535000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmed.2023.1236948\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,8]]},"references-count":26,"alternative-id":["10.3389\/fmed.2023.1236948"],"URL":"https:\/\/doi.org\/10.3389\/fmed.2023.1236948","relation":{},"ISSN":["2296-858X"],"issn-type":[{"value":"2296-858X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,8]]},"article-number":"1236948"}}