{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T20:26:34Z","timestamp":1784751994260,"version":"3.55.0"},"reference-count":55,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T00:00:00Z","timestamp":1694131200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Little is known about the prognostic ability of nCD64 in critically ill patients. This study aimed to assess the prognostic values of nCD64 in adult ICU patients with sepsis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A prospective cohort study was conducted at the ICU of Cho Ray Hospital in Vietnam between January 2019 to September 2020. All newly admitted 86 septic patients diagnosed based on sepsis-3 criteria were included. An evaluation of nCD64 was performed at admission (T0) and 48\u2009h thereafter (T48). Delta nCD64 (nCD64 T48 \u2013 nCD64 T0), %delta nCD64 [(nCD64 T48 \u2013 nCD64 T0)\/nCD64 T0 x 100%], APACHE II and SOFA scores were calculated and examined. Serum procalcitonin levels and white blood cell counts were documented. Spearman\u2019s rank correlation coefficient was used to test the correlation between nCD64 and severity scores. Receiver-operating characteristic (ROC) curve was performed to evaluate the predictive efficacy of the sepsis parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Patients with septic shock had significantly higher nCD64 levels than septic patients [3,568 (2,589; 5,999) vs. 1,514 (1,416;2,542) molecules\/cell, <jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.001]. nCD64 T0 and SOFA scores had a moderately positive linear correlation (<jats:italic>R<\/jats:italic>\u2009=\u20090.31, <jats:italic>p<\/jats:italic>\u2009=\u20090.004). In the survivor group, nCD64 levels significantly decreased within the first 48\u2009h of admission (<jats:italic>p<\/jats:italic>\u2009&amp;lt;\u20090.001), while this trend was not statistically significant in the non-survivor group (<jats:italic>p<\/jats:italic>\u2009=\u20090.866). The area under the ROC curve (AUC) value of %delta nCD64 combined with APACHE II score (0.81) was higher than that of any other parameter alone or in combination with each other.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The nCD64 index may serve as a valuable biomarker for predicting the course of sepsis. Monitoring changes in nCD64 during the initial 48\u2009h of admission can aid in predicting the prognosis of septic patients. The use of a combination of the trends of nCD64 index in the first 48\u2009h with APACHE II score would further enhance the predictive accuracy. More studies with longer follow-ups are needed to fully understand the implications of serial trend and kinetics of nCD64 in septic patients.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmed.2023.1251221","type":"journal-article","created":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T10:17:37Z","timestamp":1694168257000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":14,"title":["Neutrophil CD64\u2013a prognostic marker of sepsis in intensive care unit: a prospective cohort study"],"prefix":"10.3389","volume":"10","author":[{"given":"Huy Minh","family":"Pham","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duy Ly Minh","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minh Cuong","family":"Duong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuan Thi","family":"Phan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linh Thanh","family":"Tran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duong Hong Thuy","family":"Trang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thao Thi Ngoc","family":"Pham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2023,9,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1001\/jama.2016.0287","article-title":"The third international consensus definitions for sepsis and septic shock (sepsis-3)","volume":"315","author":"Singer","year":"2016","journal-title":"JAMA"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/S0140-6736(19)32989-7","article-title":"Global, regional, and national sepsis incidence and mortality, 1990-2017: analysis for the global burden of disease study","volume":"395","author":"Rudd","year":"2020","journal-title":"Lancet"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1186\/s13054-020-02950-2","article-title":"Mortality in sepsis and septic shock in Europe, North America and Australia between 2009 and 2019\u2013results from a systematic review and meta-analysis","volume":"24","author":"Bauer","year":"2020","journal-title":"Crit Care"},{"key":"ref4","doi-asserted-by":"publisher","first-page":"1107","DOI":"10.1164\/rccm.202112-2743OC","article-title":"Epidemiology, management, and outcomes of Sepsis in intensive care units among countries of differing National Wealth across Asia","volume":"206","author":"Li","year":"2022","journal-title":"Am J Respir Crit Care Med"},{"key":"ref5","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1097\/CCM.0000000000005357","article-title":"Surviving Sepsis 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European working group on clinical cell analysis","volume":"33","author":"Gratama","year":"1998","journal-title":"Cytometry"},{"key":"ref35","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1002\/cyto.b.20359","article-title":"Performance of calibration standards for antigen quantitation with flow cytometry in chronic lymphocytic leukemia","volume":"72","author":"Rossmann","year":"2007","journal-title":"Cytometry B Clin Cytom"},{"key":"ref36","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1148\/radiology.143.1.7063747","article-title":"The meaning and use of the area under a receiver operating characteristic (ROC) curve","volume":"143","author":"Hanley","year":"1982","journal-title":"Radiology"},{"key":"ref37","author":"Nguyen","year":"2017"},{"key":"ref38","year":"2020"},{"key":"ref39","doi-asserted-by":"publisher","first-page":"13961","DOI":"10.1111\/jcmm.16004","article-title":"Differential expression of CD64 in patients with Mycobacterium tuberculosis infection: a potential biomarker for clinical diagnosis and prognosis","volume":"24","author":"Liu","year":"2020","journal-title":"J Cell Mol Med"},{"key":"ref40","doi-asserted-by":"publisher","first-page":"6214363","DOI":"10.1155\/2020\/6214363","article-title":"Valuable role of neutrophil CD64 and highly sensitive CRP biomarkers for diagnostic, monitoring, and prognostic evaluations of Sepsis patients in neonatal ICUs","volume":"2020","author":"Hashem","year":"2020","journal-title":"BioMed Res Int"},{"key":"ref41","doi-asserted-by":"publisher","first-page":"2776","DOI":"10.1177\/1178633719862776","article-title":"Lactate predicts both Short-and long-term mortality in patients with and without Sepsis","volume":"12","author":"Villar","year":"2019","journal-title":"Infect Dis"},{"key":"ref42","doi-asserted-by":"publisher","first-page":"591","DOI":"10.5005\/jp-journals-10071-23935","article-title":"Study of significance of serum lactate kinetics in Sepsis as mortality predictor","volume":"26","author":"Chaudhari","year":"2022","journal-title":"Indian J Crit Care Med"},{"key":"ref43","doi-asserted-by":"publisher","first-page":"e0252206","DOI":"10.1371\/journal.pone.0252206","article-title":"Evaluation of leukopenia during sepsis as a marker of sepsis-defining organ dysfunction","volume":"16","author":"Belok","year":"2021","journal-title":"PLoS One"},{"key":"ref44","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1186\/s13054-015-0972-z","article-title":"Neutrophil CD64 expression as a diagnostic marker for sepsis in adult patients: a meta-analysis","volume":"19","author":"Wang","year":"2015","journal-title":"Crit Care"},{"key":"ref45","doi-asserted-by":"publisher","first-page":"1427","DOI":"10.3390\/diagnostics13081427","article-title":"Diagnostic value of neutrophil CD64 in Sepsis patients in the intensive care unit: a cross-sectional study","volume":"13","author":"Pham","year":"2023","journal-title":"Diagnostics"},{"key":"ref46","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1111\/j.1440-1843.2010.01876.x","article-title":"Comparison of Fcgamma receptor expression on neutrophils with procalcitonin for the diagnosis of sepsis in critically ill patients","volume":"16","author":"Hsu","year":"2011","journal-title":"Respirology"},{"key":"ref47","doi-asserted-by":"publisher","first-page":"569","DOI":"10.4103\/ijccm.IJCCM_228_18","article-title":"Correlation of neutrophil CD64 with clinical profile and outcome of Sepsis patients during intensive care unit stay","volume":"22","author":"Ghosh","year":"2018","journal-title":"Indian J Crit Care Med"},{"key":"ref48","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/s13613-015-0058-8","article-title":"Timing and causes of death in septic shock","volume":"5","author":"Daviaud","year":"2015","journal-title":"Ann Intensive 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