{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T16:00:36Z","timestamp":1753891236216,"version":"3.41.2"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T00:00:00Z","timestamp":1728604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p>Anthrax is an acute zoonotic infectious disease caused by <jats:italic>Bacillus anthracis<\/jats:italic>. It is categorized as a Class B (reported within 24 h of onset, including pulmonary anthrax, which is managed as a Class A infectious disease and reported within 2 h of onset) infectious disease in China. Human anthrax infection primarily occurs through direct or indirect contact with infected animals. This study reports a case of cutaneous anthrax where typical anthrax colonies were observed in conventional microbial cultures, and large Gram-positive rods with squared ends were visible under the microscope. The results from metagenomic next-generation sequencing (mNGS) suggested the presence of <jats:italic>Bacillus anthracis<\/jats:italic>. This research explores the value of combining traditional microbiology with mNGS technology for the early diagnosis and therapy of infectious diseases.<\/jats:p>","DOI":"10.3389\/fmed.2024.1440130","type":"journal-article","created":{"date-parts":[[2024,10,11]],"date-time":"2024-10-11T04:28:17Z","timestamp":1728620897000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Case report: A case of cutaneous anthrax guided by metagenomic next-generation sequencing 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