{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T17:19:24Z","timestamp":1758043164630,"version":"3.44.0"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p>The Nocardia genus is an aerobic, Gram positive, opportunistic pathogen that primarily affects cell-mediated immunosuppressed patients. The clinical manifestation of nocardiosis varies widely, making it challenging to diagnose. In this report, we describe a 48\u202fyear old woman with a muscular abscess caused by <jats:italic>Nocardia asteroides<\/jats:italic>. Venous blood, skin biopsy specimen, muscle tissue, and inguinal lymph node puncture tissue cultures yielded negative results. Using metagenomic next-generation sequencing (mNGS), the pathogen was identified as <jats:italic>Nocardia asteroides<\/jats:italic>. Whole-exome sequencing of the peripheral blood showed that the patient had a monoallelic mutation in the lipopolysaccharide-responsive beige-like anchor protein (LRBA) gene. The mNGS detected <jats:italic>Nocardia asteroides<\/jats:italic> in the patient, and the administration of accurate treatment led to her complete recovery.<\/jats:p>","DOI":"10.3389\/fmed.2025.1626685","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T04:16:06Z","timestamp":1757650566000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Case Report: Diagnosis of Nocardia asteroides infection using metagenomic next-generation sequencing of lymph node puncture tissue"],"prefix":"10.3389","volume":"12","author":[{"given":"Ya Min","family":"Yang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning 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