{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:28:43Z","timestamp":1792664923388,"version":"4.5.0"},"reference-count":36,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T00:00:00Z","timestamp":1753056000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Androgenic alopecia (AGA) is the most common hair loss disease, caused by a combination of genetic factors and hormonal imbalances. Platelet-rich plasma (PRP) therapy has gained significant recognition in treating AGA, but optimal treatment protocol regarding centrifugation techniques has yet to reach a standardized consensus. We aim to conduct a systematic review and meta-analysis of randomized controlled trials (RCTs) to compare the efficacy of single-spin versus double-spin centrifugation protocols for PRP in patients with AGA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We searched PubMed, Embase, and Cochrane for RCTs comparing single-spin to double-spin PRP in AGA patients. The primary endpoint was the final platelets count after centrifugation. Secondary endpoints included improvement in hair density, as well as terminal and vellus hair rate. Statistical analysis was performed using RStudio software. Heterogeneity was assessed with <jats:italic>I<\/jats:italic><jats:sup>2<\/jats:sup> statistics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We included 90 participants with AGA from three RCTs. The pooled mean difference (MD) for platelets concentration was 66.14 in single-spin compared to double-spin PRP (95% CI: \u2212372.96 to 505.25; <jats:italic>p<\/jats:italic> = 0.77; <jats:italic>I<\/jats:italic><jats:sup>2<\/jats:sup> = 83.5%). The MD for change in hair density was 4.10% in single versus double-spin (95% CI: \u22124.74 to 12.93; <jats:italic>p<\/jats:italic> = 0.36; <jats:italic>I<\/jats:italic><jats:sup>2<\/jats:sup> = 0%). Moreover, the MD for terminal and vellus hair after single-spin versus double-spin PRP was 1.16 (95% CI: \u22123.34 to 5.66; <jats:italic>p<\/jats:italic> = 0.61; <jats:italic>I<\/jats:italic><jats:sup>2<\/jats:sup> = 0%), and \u22123.59 (95% CI: \u221212.49 to 5.30; <jats:italic>p<\/jats:italic> = 0.45; <jats:italic>I<\/jats:italic><jats:sup>2<\/jats:sup> = 4.8%), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study suggests that single-spin PRP centrifugation is better in treating AGA patients compared to double-spin PRP.<\/jats:p><\/jats:sec><jats:sec><jats:title>Systematic review registration<\/jats:title><jats:p><jats:ext-link>https:\/\/www.crd.york.ac.uk\/PROSPERO\/view\/CRD42025643296<\/jats:ext-link>, identifier CRD42025643296.<\/jats:p><\/jats:sec>","DOI":"10.3389\/fmed.2025.1631087","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T04:10:37Z","timestamp":1753071037000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":9,"title":["Comparison of single-spin to double-spin platelet-rich plasma centrifugation methods in the treatment of androgenic alopecia: a systematic review and meta-analysis of randomized controlled trials"],"prefix":"10.3389","volume":"12","author":[{"given":"Laura","family":"Ghanem","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najwaa","family":"Kirmani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Paula","family":"Palacios-Ortiz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Cevallos-Cueva","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela Luc\u00eda","family":"Mendoza-Mill\u00e1n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel Victor","family":"Almeida Nascimento","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Virginia","family":"Velasco-Tamariz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2025,7,21]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1433962","DOI":"10.3389\/fnut.2024.1433962","article-title":"Androgenic alopecia is associated with higher dietary inflammatory index and lower antioxidant index scores.","volume":"11","author":"Bazmi","year":"2024","journal-title":"Front Nutr."},{"key":"B2","doi-asserted-by":"publisher","first-page":"158","DOI":"10.1016\/j.tibtech.2008.11.009","article-title":"Classification of platelet concentrates: From pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF).","volume":"27","author":"Dohan Ehrenfest","year":"2009","journal-title":"Trends Biotechnol."},{"key":"B3","doi-asserted-by":"publisher","first-page":"1040","DOI":"10.1111\/j.1524-4725.2012.02394.x","article-title":"Autologous platelet-rich plasma: A potential therapeutic tool for promoting hair growth.","volume":"38","author":"Li","year":"2012","journal-title":"Dermatol Surg."},{"key":"B4","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1016\/j.abd.2024.01.002","article-title":"Is autologous platelet-rich plasma capable of increasing hair density in patients with androgenic alopecia? 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