{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:44:27Z","timestamp":1790700267656,"version":"4.1.0"},"reference-count":18,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T00:00:00Z","timestamp":1766016000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100016308","name":"Wuxi Health and Family Planning Commission","doi-asserted-by":"publisher","award":["Z202301"],"award-info":[{"award-number":["Z202301"]}],"id":[{"id":"10.13039\/501100016308","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The cervicovaginal microbiome\u2014spanning from the vagina to endometrium\u2014remains undercharacterized in endometriosis-associated infertility. Objective: To determine whether combined vaginal and cervical microbial profiles predict frozen embryo transfer (FET) outcomes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In 22 endometriosis patients undergoing FET, paired vaginal and cervical samples were collected on transfer day. 16S rDNA sequencing quantified microbial composition; alpha\/beta diversity, PCoA, LEfSe, and PICRUSt analyses identified taxonomic and functional signatures linked to implantation success. Conduct a differential analysis of microorganisms in different body parts through DMI.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Microbial profiles associated with successful pregnancies featured a higher relative abundance of\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      and\n                      <jats:italic>Bifidobacterium<\/jats:italic>\n                      , whereas\n                      <jats:italic>Gardnerella, Streptococcus<\/jats:italic>\n                      , and\n                      <jats:italic>Atopobiu<\/jats:italic>\n                      m were more enriched in failures. Cervical alpha diversity was significantly lower in successful transfers. LEfSe highlighted differential taxa including\n                      <jats:italic>Peptostreptococcales<\/jats:italic>\n                      in successes and\n                      <jats:italic>Pseudomonadaceae<\/jats:italic>\n                      in failures. Functional inference predicted dysregulated metabolic pathways in failure-associated communities. Furthermore, the cervical microbiota exhibited higher DMI, indicating greater individual specificity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our pilot findings suggest that a continuous cervicovaginal microbial ecosystem presents distinct taxonomic and functional patterns associated with FET success in endometriosis. Specifically, cervical microbiota profiling emerges as a promising, minimally invasive approach worthy of further investigation to potentially personalize ART strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmed.2025.1642770","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T06:41:22Z","timestamp":1766040082000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":4,"title":["A pilot study of cervicovaginal microbiome patterns associated with embryo implantation outcomes in endometriosis-associated 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