{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T07:03:25Z","timestamp":1772435005385,"version":"3.50.1"},"reference-count":50,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T00:00:00Z","timestamp":1772409600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>This study aimed to evaluate the potential of microRNA (miRNA)-210 as a biomarker for distinguishing iron deficiency anemia (IDA) from functional iron deficiency (FID) in hemodialysis (HD) patients. The diagnostic performance of miRNA-210 was also compared with conventional biochemical markers, including hemoglobin (Hb), ferritin, transferrin saturation (TSAT), and zinc protoporphyrin (ZnPP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Fifty HD patients were classified into control, IDA, and FID groups according to Hb, ferritin, and TSAT criteria. Pre-dialysis blood samples were collected, and plasma miRNA-210 levels were measured using reverse transcription quantitative polymerase chain reaction (RT\n                      <jats:sup>2<\/jats:sup>\n                      -PCR). Diagnostic performance was assessed through receiver operating characteristic (ROC) analysis alongside traditional biomarkers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Plasma miRNA-210 levels were significantly higher in the IDA group compared to both the control (\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.0010) and FID (\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.0007) groups. A significant negative correlation was observed between miRNA-210 and Hb (\n                      <jats:italic>\u03c1<\/jats:italic>\n                      \u202f=\u202f\u22120.363,\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.0155). ROC analysis showed that miRNA-210 had moderate diagnostic discriminatory ability for differentiating IDA (AUC\u202f=\u202f0.711,\n                      <jats:italic>p<\/jats:italic>\n                      \u202f=\u202f0.0186). Its performance was comparable to ZnPP and exceeded to ferritin and TSAT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>miRNA-210 may serve as a supportive biomarker, reflecting the interaction between hypoxia and iron metabolism in distinguishing IDA from FID among HD patients. These findings indicate that miRNA-210 could provide additional value in understanding anemia pathophysiology and enhance diagnostic evaluation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Limitations<\/jats:title>\n                    <jats:p>Key limitations include the small sample size, single-center, cross-sectional design, absence of a healthy control group, and lack of molecular-level functional validation. Larger multicenter studies are needed to confirm these findings and determine clinically relevant cut-off values for miRNA-210.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmed.2026.1668328","type":"journal-article","created":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T06:35:21Z","timestamp":1772433321000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["miRNA-210 expression is associated with iron deficiency and biochemical parameters in hemodialysis patients"],"prefix":"10.3389","volume":"13","author":[{"given":"Merve","family":"K\u0131l\u0131\u00e7","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University","place":["Istanbul, T\u00fcrkiye"]},{"name":"Department of Anesthesia Program, Advanced Vocational School, Dogus University","place":["Istanbul, T\u00fcrkiye"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hamad","family":"Dheir","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Sakarya University Faculty of Medicine","place":["Sakarya, T\u00fcrkiye"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmud","family":"\u0130slam","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Sakarya University Faculty of Medicine","place":["Sakarya, T\u00fcrkiye"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zafer","family":"Ercan","sequence":"additional","affiliation":[{"name":"Department of Nephrology, Sakarya University Training and Research Hospital","place":["Sakarya, T\u00fcrkiye"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhittin Abdulkadir","family":"Serdar","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Graduate School of Health Sciences, Acibadem Mehmet Ali Aydinlar University","place":["Istanbul, T\u00fcrkiye"]},{"name":"Department of Medical Biochemistry, School of Medicine, Acibadem Mehmet Ali Aydinlar University","place":["Istanbul, T\u00fcrkiye"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,3,2]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"44","DOI":"10.1159\/000496492","article-title":"Iron deficiency anemia in chronic kidney disease","volume":"142","author":"Gafter-Gvili","year":"2019","journal-title":"Acta Haematol"},{"key":"ref2","doi-asserted-by":"crossref","DOI":"10.7759\/cureus.77598","article-title":"Iron-deficiency anemia in chronic kidney disease: a literature review of its pathophysiology, diagnosis, and management","author":"Kazmi","year":"2025","journal-title":"Cureus"},{"key":"ref3","first-page":"1","article-title":"Clinical practice guideline for the evaluation and management of chronic kidney 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