{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T15:16:34Z","timestamp":1783350994197,"version":"3.54.6"},"reference-count":46,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:00:00Z","timestamp":1782950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p>\n                    Renal ischemia-reperfusion injury (IRI) is a leading cause of acute kidney injury, with limited effective therapeutic options. While microRNAs (miRNAs) play crucial roles in regulating various diseases, including renal IRI, their specific targets and mechanisms remain elusive. In this study, hypoxia\/reoxygenation (H\/R) significantly promoted apoptosis in both NRK-52E and HK-2 cells, accompanied by a marked downregulation of miR-144-3p in both H\/R-treated cells and the kidneys of mice subjected to ischemia\/reperfusion (I\/R) injury. Moreover, miR-144-3p overexpression enhanced cell viability under H\/R conditions, as evidenced by the results of MTT assays. Dual-luciferase reporter assays identified\n                    <jats:italic>EZH2<\/jats:italic>\n                    as a direct target of miR-144-3p, while Western blot analysis revealed that miR-144-3p suppressed EZH2 protein expression. Furthermore,\n                    <jats:italic>EZH2<\/jats:italic>\n                    knockdown reduced\n                    <jats:italic>Bax<\/jats:italic>\n                    and cleaved Caspase-3 expression, highlighting its role in apoptosis regulation. Finally, miR-144-3p protected against renal IRI by inhibiting apoptosis and promoting cell proliferation by modulating EZH2, offering novel insights into its protective mechanisms. These findings position EZH2 as a potential therapeutic target for mitigating renal IRI.\n                  <\/jats:p>","DOI":"10.3389\/fmed.2026.1745179","type":"journal-article","created":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T11:10:15Z","timestamp":1782990615000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["MiR-144-3p regulates cell proliferation and apoptosis in renal ischemia-reperfusion injury by targeting EZH2"],"prefix":"10.3389","volume":"13","author":[{"given":"Lifeng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Urology, Ezhou Centre Hospital","place":["Ezhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuxin","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Urology, Wuhan No.1 Hospital (Traditional Chinese and Western Medicine Hospital of Wuhan), Tongji Medical College, Huazhong University of Science and Technology","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology, Wuhan No.1 Hospital (Traditional Chinese and Western Medicine Hospital of Wuhan), Tongji Medical College, Huazhong University of Science and Technology","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Urology, Wuhan No.1 Hospital (Traditional Chinese and Western Medicine Hospital of Wuhan), Tongji Medical College, Huazhong University of Science and Technology","place":["Wuhan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaping","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Urology, Ezhou Centre Hospital","place":["Ezhou, 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