{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T10:07:47Z","timestamp":1771927667816,"version":"3.50.1"},"reference-count":49,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T00:00:00Z","timestamp":1769558400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"\n Introduction<\/jats:title>\n Infectious mononucleosis (IM) is a childhood infectious disease caused by the Epstein\u2013Barr virus (EBV). Its pathogenesis is associated with immune and gut microbiota disorders. Exploring the regulatory factors of immune function and gut microbiota in IM may provide novel strategies for the prophylaxis and management of this condition. This study explored the association between 25-hydroxyvitamin D (25(OH)D) level and gut microbiota abundance in children with IM.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Individuals meeting the inclusion criteria for IM were enrolled, and their blood samples were collected. The clinical manifestations, 25(OH)D levels, cytokines, and lymphocyte subsets were selected for correlation analysis. A total of 99 participants were divided into three groups, based on the level of 25(OH)D. Sixty participants completed fecal collection, including those with suboptimal 25(OH)D (\n n<\/jats:italic>\n \u202f=\u202f29) and optimal 25(OH)D (\n n<\/jats:italic>\n \u202f=\u202f31). The V3\u2013V4 region of the 16S rRNA gene of fecal microbiota was sequenced, bioinformatics analysis was performed, and the association between the relative abundances and 25(OH)D level was analyzed.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The 25(OH)D level was positively correlated with the percentages of NK cells and B cells, and negatively correlated with IL-1\n \u03b2<\/jats:italic>\n , IL-12, IL-17, TNF-\n \u03b1<\/jats:italic>\n , T cells, and CTL cells (all\n p<\/jats:italic>\n \u202f&lt;\u202f0.01). Compared with the optimal 25(OH)D group, the 25(OH)D suboptimal group showed higher relative abundances of\n Bacillota<\/jats:italic>\n and\n Proteobacteria,<\/jats:italic>\n and lower relative abundances of\n Bacteroidetes<\/jats:italic>\n and\n Actinobacteria<\/jats:italic>\n r. Compared with the optimal group, the relative abundances of\n Acinetobacter<\/jats:italic>\n (LDA\u202f=\u202f2.53,\n p<\/jats:italic>\n \u202f=\u202f0.02),\n Epulopiscium<\/jats:italic>\n (LDA\u202f=\u202f2.76,\n p<\/jats:italic>\n \u202f=\u202f0.02), and\n Collinsella<\/jats:italic>\n (LDA\u202f=\u202f3.16,\n p<\/jats:italic>\n \u202f=\u202f0.04) increased, while the relative abundances of\n Anaerostipes<\/jats:italic>\n (LDA\u202f=\u202f2.76,\n p<\/jats:italic>\n \u202f=\u202f0.042),\n Helicobacter<\/jats:italic>\n (LDA\u202f=\u202f2.79,\n p<\/jats:italic>\n \u202f=\u202f0.041), and\n Desulfovibrio<\/jats:italic>\n (LDA\u202f=\u202f2.76,\n p<\/jats:italic>\n \u202f=\u202f0.02) decreased in suboptimal group.\n Anaerostipes<\/jats:italic>\n was positively correlated with 25(OH)D level (\n r<\/jats:italic>\n \u202f=\u202f0.335,\n p<\/jats:italic>\n \u202f&lt;\u202f0.001), whereas\n Acinetobacter<\/jats:italic>\n was negatively correlated (\n r<\/jats:italic>\n \u202f=\u202f\u22120.303,\n p<\/jats:italic>\n \u202f&lt;\u202f0.001).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n The level of 25(OH)D was associated with gut microbiota and immune function in children with IM. Improving vitamin D deficiency may help maintain normal gut microbiota, ameliorate immune dysregulation, and reduce IM-related complications.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fmed.2026.1755236","type":"journal-article","created":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T06:45:45Z","timestamp":1769582745000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Gut microbiota interactions with the immunomodulatory role of 25-hydroxyvitamin D in children with infectious mononucleosis"],"prefix":"10.3389","volume":"13","author":[{"given":"Xiangyu","family":"Chen","sequence":"first","affiliation":[{"name":"The Ward I of Pediatric, Jinhua Maternal and Child Health Care Hospital","place":["Zhejiang, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Huang","sequence":"additional","affiliation":[{"name":"The Affiliated Jinhua Hospital of Wenzhou Medical University","place":["Zhejiang, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiandong","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Hematology, Yuyao People\u2019s Hospital of Zhejiang Province","place":["Ningbo, Zhejiang, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,1,28]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"e70421","DOI":"10.1002\/jmv.70421","article-title":"Development of a diagnostic algorithm for Epstein-Barr virus-related diseases: a retrospective observational study","volume":"97","author":"Li","year":"2025","journal-title":"J Med Virol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1007\/s10875-024-01701-0","article-title":"Single-cell transcriptomic analysis of Epstein-Barr virus-associated hemophagocytic 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