{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T06:08:41Z","timestamp":1775628521184,"version":"3.50.1"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Red blood cell (RBC) alloimmunization remains a significant challenge in patients requiring repeated transfusions, complicating transfusion procedures and increasing healthcare resource utilization. While extended Rh antigen matching has been proposed to mitigate alloimmunization, prospective data on its impact on transfusion-related outcomes and clinical management remain limited.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this prospective, randomized controlled study, patients requiring multiple RBC transfusions were randomly assigned to receive either conventional antigen matching or Rh five-antigen (D, C, c, E, e)\u2013matched transfusions. The primary endpoint was the incidence of RBC alloantibody formation. Secondary endpoints included transfusion-related adverse reactions, as well as transfusion management outcomes such as RBC utilization, transfusion intervals, and length of hospital stay. Multivariable analyses were performed to adjust for potential confounders.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Patients in the Rh five-antigen\u2013matched group demonstrated a significantly lower incidence of alloantibody formation and fewer transfusion-related adverse reactions compared to the conventional matching group. Additionally, Rh five-antigen matching was associated with reduced RBC utilization, longer transfusion intervals, and shorter hospital stays. After adjusting for relevant clinical variables, Rh five-antigen\u2013matched transfusion remained independently associated with lower RBC utilization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In patients requiring repeated RBC transfusions, Rh five-antigen\u2013matched transfusion was associated with a reduced risk of alloantibody formation and more favorable transfusion management outcomes. This strategy represents a viable transfusion management approach for patients at high immunological risk, and its long-term clinical value and cost-effectiveness should be further explored in multicenter studies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmed.2026.1795865","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T05:31:48Z","timestamp":1775626308000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of Rh five-antigen\u2013matched transfusion on alloimmunization and clinical outcomes in patients requiring repeated red blood cell transfusions: a prospective randomized controlled study"],"prefix":"10.3389","volume":"13","author":[{"given":"Xiaolu","family":"Xie","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine, Affiliated Hospital of Yangzhou University","place":["Yangzhou, Jiangsu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ye","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Affiliated Hospital of Yangzhou University","place":["Yangzhou, Jiangsu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanke","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Affiliated Hospital of Yangzhou University","place":["Yangzhou, Jiangsu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine, Affiliated Hospital of Yangzhou University","place":["Yangzhou, Jiangsu, China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2026,4,8]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1016\/j.tracli.2006.02.011","article-title":"Molecular genetics of RH and its clinical application","volume":"13","author":"Flegel","year":"2006","journal-title":"Transfus Clin Biol"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1111\/bjh.15182","article-title":"Risk factors for red blood cell alloimmunization in the recipient epidemiology and donor evaluation study (REDS-III) database","volume":"181","author":"Karafin","year":"2018","journal-title":"Br J Haematol"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"1821","DOI":"10.1182\/blood-2018-08-833962","article-title":"Transfusion-related red blood cell alloantibodies: induction and 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