{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:52:50Z","timestamp":1785909170619,"version":"3.56.0"},"reference-count":26,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T00:00:00Z","timestamp":1785888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p>\n                    Extrapulmonary tuberculosis (EPTB) is characterized by low bacterial load and complex matrix in lesion samples. Therefore, current tuberculosis (TB) diagnostic methods often lose sensitivity to detect\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    (\n                    <jats:italic>Mtb<\/jats:italic>\n                    ), leading to missed diagnosis and delayed treatment. Herein, we presented a single-cell metabolic labeling probe for tuberculosis (SCMLP-TB), which exhibited supporting investigative evidence for diagnosis of renal TB case, and enabled investigative monitoring of therapeutic efficacy of anti-TB regimens. A 51-year-old patient presented with fever, generalized weakness and muscle soreness on admission. Chest computed tomography (CT) revealed calcified mediastinal lymph nodes, and relevant renal function indicators were abnormal. Conventional TB diagnostic methods, including Xpert MTB\/RIF, TB-DNA testing, and culture, yielded negative results using sputum, blood, and urine samples. Notably, SCMLP-TB assay detected\n                    <jats:italic>Mtb<\/jats:italic>\n                    in urine samples, suspecting renal TB. SCMLP-TB assay further detected gradually decreased bacterial viability along with anti-TB treatment (isoniazid, rifapentine, ethambutol, and pyrazinamide), revealing the efficacy of current regimen. The patient\u2019s symptom resolution and anemia\/renal function improvement provided auxiliary evidence of antimicrobial efficacy. Ultimately, SCMLP-TB showed great potential for non-invasive, sensitive detection of suspected renal TB, and enabled investigative monitoring of the therapeutic efficacy.\n                  <\/jats:p>","DOI":"10.3389\/fmed.2026.1849446","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:41:39Z","timestamp":1785908499000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Case Report: Single-cell metabolic labeling probe for diagnosing renal tuberculosis"],"prefix":"10.3389","volume":"13","author":[{"given":"Yuanyuan","family":"Zou","sequence":"first","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiqin","family":"Dai","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiling","family":"Liu","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Lin","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deliang","family":"Liu","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengli","family":"Xu","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqiang","family":"Lin","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiafei","family":"Dai","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]},{"name":"Molecular Biology Research Center and Center for Medical Genetics, School of Life Sciences, Central South University","place":["Changsha, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingbin","family":"Zheng","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]},{"name":"The Affiliated Dongguan Songshan Lake Central Hospital, Guangdong Medical University","place":["Dongguan, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhou","family":"Lu","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]},{"name":"Shenzhen Key Laboratory for Infectious Diseases, Shenzhen Third People\u2019s Hospital","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Clinical Research Center for Infectious Disease, Shenzhen Third People\u2019s Hospital, Southern University of Science and Technology","place":["Shenzhen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,8,5]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","first-page":"250174","DOI":"10.1183\/16000617.0174-2025","article-title":"Host- and pathogen-related determinants of pulmonary versus extrapulmonary tuberculosis","volume":"35","author":"Tulu","year":"2026","journal-title":"Eur Respir Rev"},{"key":"ref2","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/j.cmi.2025.09.002","article-title":"Diagnostic performance of Xpert MTB\/RIF ultra assay with pulmonary and extrapulmonary specimens: a retrospective evaluation in a low-incidence setting in Finland","volume":"32","author":"Luukinen","year":"2026","journal-title":"Clin Microbiol Infect"},{"key":"ref3","doi-asserted-by":"publisher","first-page":"103001","DOI":"10.1016\/j.eucr.2025.103001","article-title":"Renal tuberculosis mimicking cystic renal cell carcinoma: a case 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