{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T05:03:49Z","timestamp":1785301429405,"version":"3.55.0"},"reference-count":24,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Platelet storage at room temperature (20\u201324\u00b0 C) with agitation facilitates bacterial proliferation. Despite global standards for culture-based screening, there is a lack of component-level contamination data within the Saudi Arabian context.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We conducted a retrospective review of 28,416 post-donation platelet components at the Riyadh regional laboratory (2020\u20132021) to determine the culture-confirmed bacterial contamination rate. Screening for bacterial contamination utilized the BacT\/ALERT\n                      <jats:sup>\u00ae<\/jats:sup>\n                      VIRTUO system at 24 \u00b1 2 h post-collection. The primary outcome was to measure the confirmed contamination rate per 1,000 components; secondary outcomes included the positive predictive value (PPV) of initial screening and organism distribution. Screen-positive units were quarantined and underwent a formal confirmation algorithm via repeat culture and subculture.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Out of 28,416 components, 49 (0.17%) were initially screen-positive. Twenty-five cases were culture-confirmed, yielding a contamination rate of 0.88 per 1,000 components (95% CI: 0.54\u20131.23) and a Positive Predictive Value (PPV) of 51.0%. Skin commensals predominated, led by\n                      <jats:italic>Staphylococcus epidermidis<\/jats:italic>\n                      (28.0%) and\n                      <jats:italic>Corynebacterium<\/jats:italic>\n                      spp. (16.0%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The confirmed contamination rate aligns with international benchmarks for primary culture programs. These findings suggest that implementing advanced strategies like Large-Volume Delayed Sampling (LVDS) or near-issue secondary testing could further reduce the residual risk posed by slow-growing skin flora.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmed.2026.1865609","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T04:47:13Z","timestamp":1785300433000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Low bacterial contamination rate in platelet components following primary culture screening: a Saudi Arabian experience"],"prefix":"10.3389","volume":"13","author":[{"given":"Mohammed Battah","family":"Alenazi","sequence":"first","affiliation":[{"name":"College of Medicine, Alfaisal University","place":["Riyadh, Saudi Arabia"]},{"name":"Riyadh Central Blood Bank, Blood Transfusion Service, Health Support Service Center, MOH","place":["Riyadh, Saudi 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University","place":["Riyadh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasha M.","family":"Khayata","sequence":"additional","affiliation":[{"name":"College of Medicine, Alfaisal University","place":["Riyadh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liyan","family":"Afaneh","sequence":"additional","affiliation":[{"name":"College of Medicine, Alfaisal University","place":["Riyadh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Badr","family":"Elwy","sequence":"additional","affiliation":[{"name":"College of Medicine, Alfaisal University","place":["Riyadh, Saudi Arabia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"000780.v3","DOI":"10.1099\/acmi.0.000780.v3","article-title":"Comparative virulome analysis of four Staphylococcus epidermidis 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