{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:54:52Z","timestamp":1791179692711,"version":"4.1.0"},"reference-count":34,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:p>\n                    This study describes a rare case of pneumonia induced by coinfection with\n                    <jats:italic>Chlamydia psittaci<\/jats:italic>\n                    (\n                    <jats:italic>C. psittaci<\/jats:italic>\n                    ) and\n                    <jats:italic>Haemophilus influenzae (H. influenzae)<\/jats:italic>\n                    . The patient had a history of chronic nephritis and long-term pigeon breeding. The initial manifestation was slurred speech, followed by rapid progression to high fever and respiratory failure. Chest computed tomography revealed bilateral interstitial pneumonia, predominantly in the right lung. Pathogen-targeted quick multiplex polymerase chain reaction (pt-qPCR) confirmed the pathogens as\n                    <jats:italic>C. psittaci<\/jats:italic>\n                    and\n                    <jats:italic>H. influenzae<\/jats:italic>\n                    , and metagenomic next-generation sequencing (mNGS) further verified consistent results. After 7-days of combination therapy with doxycycline and azithromycin, the treatment was switched to doxycycline combined with moxifloxacin for an additional 8-days. The patient achieved complete recovery and was discharged from the hospital. This case suggests that pt-qPCR may facilitate early identification of mixed infections in patients with severe pneumonia and a history of avian exposure, thereby providing valuable information to guide clinical decision-making.\n                  <\/jats:p>","DOI":"10.3389\/fmed.2026.1890644","type":"journal-article","created":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T05:31:13Z","timestamp":1791178273000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Pathogen-targeted quick multiplex PCR identification of Chlamydia psittaci and Haemophilus influenzae coinfection in pneumonia: a case report"],"prefix":"10.3389","volume":"13","author":[{"given":"Ying","family":"Yuan","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, Liaocheng Third People\u2019s Hospital","place":["Liaocheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Liaocheng Third People\u2019s Hospital","place":["Liaocheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Liaocheng Third People\u2019s Hospital","place":["Liaocheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Liaocheng Third People\u2019s Hospital","place":["Liaocheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Critical Care Medicine, Liaocheng Third People\u2019s Hospital","place":["Liaocheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youxin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Liaocheng Third People\u2019s Hospital","place":["Liaocheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunfeng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory, Liaocheng Third People\u2019s Hospital","place":["Liaocheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingzhao","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, Liaocheng Tumor Hospital","place":["Liaocheng, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,10,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"1249225","DOI":"10.3389\/fcimb.2023.1249225","article-title":"Case report: metagenomic next-generation sequencing applied in diagnosing psittacosis caused by chlamydia psittaci infection","volume":"13","author":"Xu","year":"2023","journal-title":"Front Cell Infect Microbiol"},{"key":"B2","doi-asserted-by":"publisher","first-page":"414","DOI":"10.1007\/s11250-021-02859-0","article-title":"A comprehensive review on avian chlamydiosis: a neglected zoonotic disease","volume":"53","author":"Ravichandran","year":"2021","journal-title":"Trop Anim Health Prod"},{"key":"B3","doi-asserted-by":"publisher","first-page":"3096","DOI":"10.1017\/s0950268817002060","article-title":"Chlamydia psittaci (psittacosis) as a cause of community-acquired pneumonia: a systematic review and meta-analysis","volume":"145","author":"Hogerwerf","year":"2017","journal-title":"Epidemiol Infect"},{"key":"B4","doi-asserted-by":"publisher","first-page":"191","DOI":"10.3760\/cma.j.issn.1674-2397.2024.03.002","article-title":"(2024). 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