{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T12:39:26Z","timestamp":1792413566411,"version":"4.4.0"},"reference-count":19,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T00:00:00Z","timestamp":1789516800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med."],"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Corneal transparency is maintained by its avascular nature, and disruption following injury can lead to corneal neovascularization (CNV), resulting in visual impairment. Although current treatment such as corticosteroids, NSAIDs, anti-VEGF agents, and surgical interventions are available, their use is often limited by adverse effects, recurrence, and cost. Jatimukuladdiv arti Anjana (JMV), a classical ayurvedic ophthalmic formulation with reported anti-inflammatory and tissue-healing properties, was evaluated for its therapeutic efficacy in an alkali burn-induced CNV model in albino rats.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Eighteen Swiss albino rats (36 eyes) were used. Corneal injury was induced in albino rats using a standardized alkali burn model. Animals were allocated to control, standard treatment, and JMV-treated groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096 rats\/group). Clinical assessments were performed at predetermined intervals using validated scoring criteria for corneal opacity, neovascularization, vessel size, corneal haze, and wound healing on days 0, 7 and 14 in a blinded manner. Histopathological analysis was conducted on day 15. In addition, liquid chromatography\u2013mass spectrometry (LC\u2013MS) analysis was performed to characterize the phytochemical profile of JMV.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>JMV treatment was associated with significant improvement in corneal healing, characterized by reduced opacity, neovascularization, vascular enlargement, and corneal haze compared with the untreated control group. Histopathological findings supported these observations, revealing attenuation of inflammatory and fibrotic changes with improved tissue organization and epithelial restoration in treated animals.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>The present study evaluated the therapeutic potential of Jatimukuladi Varti Anjana (JMV) in an experimental model of alkali burn-induced corneal injury and neovascularization in albino rats. The findings demonstrated that JMV significantly improved corneal healing, as evidenced by reduced corneal opacity, neovascularization, vessel size, and corneal haze compared with the untreated control group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>JMV demonstrated significant anti-angiogenic and wound-healing potential in alkali-burn-induced CNV. These findings support its potential role as an integrative therapeutic option for corneal neovascularisation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.3389\/fmed.2026.1921154","type":"journal-article","created":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T04:29:41Z","timestamp":1789532981000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic potential of Jatimukuladi Varti Anjana in alkali burn-induced corneal injury and neovascularization in albino rats"],"prefix":"10.3389","volume":"13","author":[{"given":"Vishva S.","family":"Pandya","sequence":"first","affiliation":[{"name":"Department of Shalakya Tantra, Amrita School of Ayurveda, Amrita Vishwa Vidyapeetham","place":["Amritapuri, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. N.","family":"Ashwini","sequence":"additional","affiliation":[{"name":"Department of Shalakya Tantra, Amrita School of Ayurveda, Amrita Vishwa Vidyapeetham","place":["Amritapuri, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Sivabalaji","sequence":"additional","affiliation":[{"name":"Department of Shalakya Tantra, Amrita School of Ayurveda, Amrita Vishwa Vidyapeetham","place":["Amritapuri, India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,9,16]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"190","DOI":"10.4103\/ijo.IJO_646_17","article-title":"Anatomy of cornea and ocular surface","volume":"66","author":"Sridhar","year":"2018","journal-title":"Indian J Ophthalmol"},{"key":"B2","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/j.semcdb.2007.08.014","article-title":"Maintaining transparency: a review of the developmental physiology and pathophysiology of two avascular tissues","volume":"19","author":"Beebe","year":"2008","journal-title":"Semin Cell Dev Biol"},{"key":"B3","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.preteyeres.2010.01.002","article-title":"Novel aspects of corneal angiogenic and lymphangiogenic privilege","volume":"29","author":"Ellenberg","year":"2010","journal-title":"Prog Retin Eye Res"},{"key":"B4","first-page":"264","article-title":"Corneal angiogenic privilege: angiogenic and antiangiogenic factors in corneal avascularity, vasculogenesis, and wound healing (an American Ophthalmological Society thesis)","volume":"104","author":"Azar","year":"2006","journal-title":"Trans Am Ophthalmol Soc"},{"key":"B5","author":"Srikanta Murthy","year":"2016"},{"key":"B6","author":"Sharma","year":"2019"},{"key":"B7","author":"Srikanta Murthy","year":"2016"},{"key":"B8","doi-asserted-by":"publisher","first-page":"e0122951","DOI":"10.1371\/journal.pone.0122951","article-title":"Therapeutic effects of topical netrin-4 inhibits corneal neovascularization in alkali-burn rats","volume":"10","author":"Han","year":"2015","journal-title":"PLoS One"},{"key":"B9","doi-asserted-by":"publisher","first-page":"51159","DOI":"10.3791\/51159","article-title":"An alkali-burn injury model of corneal neovascularization in the mouse","volume":"86","author":"Anderson","year":"2014","journal-title":"J Vis Exp"},{"key":"B10","doi-asserted-by":"publisher","first-page":"2628","DOI":"10.3390\/molecules13102628","article-title":"Anti-carcinogenic effects of the flavonoid luteolin","volume":"13","author":"Seelinger","year":"2008","journal-title":"Molecules"},{"key":"B11","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1006\/exer.2000.0900","article-title":"Treatment of corneal neovascularization with dietary isoflavonoids and flavonoids","volume":"71","author":"Joussen","year":"2000","journal-title":"Exp Eye Res"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1336","DOI":"10.3389\/fphar.2019.01336","article-title":"Molecular mechanisms of action of genistein in cancer: recent advances","volume":"10","author":"Tuli","year":"2019","journal-title":"Front Pharmacol"},{"key":"B13","doi-asserted-by":"publisher","first-page":"226","DOI":"10.1016\/j.canlet.2008.03.052","article-title":"Multi-targeted therapy of cancer by genistein","volume":"269","author":"Banerjee","year":"2008","journal-title":"Cancer Lett"},{"key":"B14","doi-asserted-by":"publisher","first-page":"408","DOI":"10.3945\/an.114.008052","article-title":"Genistein and cancer: current status, challenges, and future directions","volume":"6","author":"Spagnuolo","year":"2015","journal-title":"Advances in Nutrition (Bethesda, Md.)"},{"key":"B15","doi-asserted-by":"publisher","first-page":"2327","DOI":"10.3390\/cells10092327","article-title":"Sinapic acid controls inflammation by suppressing NLRP3 inflammasome activation","volume":"10","author":"Lee","year":"2021","journal-title":"Cells"},{"key":"B16","doi-asserted-by":"publisher","first-page":"e0205338","DOI":"10.1371\/journal.pone.0205338","article-title":"Topical anti-inflammatory activity of palmitoleic acid improves wound healing","volume":"13","author":"Weimann","year":"2018","journal-title":"PLoS One"},{"key":"B17","doi-asserted-by":"publisher","first-page":"987","DOI":"10.1038\/193987a0","article-title":"Fusidic acid: a new antibiotic","volume":"193","author":"Godtfredsen","year":"1962","journal-title":"Nature"},{"key":"B18","doi-asserted-by":"publisher","first-page":"3592","DOI":"10.3390\/molecules30173592","article-title":"The role of nutraceuticals in age-related ocular diseases","volume":"30","author":"Starvaggi","year":"2025","journal-title":"Molecules"},{"key":"B19","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1016\/j.imbio.2010.06.007","article-title":"Oleic acid modulation of the immune response in wound healing: a new approach for skin repair","volume":"216","author":"Cardoso","year":"2011","journal-title":"Immunobiology"}],"updated-by":[{"DOI":"10.3389\/fmed.2026.2023065","type":"correction","label":"Correction","source":"publisher","updated":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T00:00:00Z","timestamp":1792368000000}}],"container-title":["Frontiers in Medicine"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmed.2026.1921154\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T11:39:32Z","timestamp":1792409972000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmed.2026.1921154\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,16]]},"references-count":19,"alternative-id":["10.3389\/fmed.2026.1921154"],"URL":"https:\/\/doi.org\/10.3389\/fmed.2026.1921154","relation":{},"ISSN":["2296-858X"],"issn-type":[{"value":"2296-858X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,16]]},"article-number":"1921154"}}