{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T06:02:50Z","timestamp":1781071370952,"version":"3.54.1"},"reference-count":10,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Med. Technol."],"abstract":"\n Background<\/jats:title>\n \n Listeria monocytogenes<\/jats:italic>\n is an opportunistic foodborne pathogen that causes severe invasive infections, such as meningitis, primarily in immunocompromised individuals, the elderly, and pregnant women. Diagnosis is often challenging due to nonspecific early symptoms.\n <\/jats:p>\n <\/jats:sec>\n \n Case description<\/jats:title>\n \n A 67-year-old male with a history of chronic obstructive pulmonary disease (COPD) presented with a 4-day history of persistent high-grade fever and altered mental status. Initial empirical antibiotic therapy (meropenem) proved ineffective.Metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) definitively identified\n L.monocytogenes<\/jats:italic>\n . The patient was diagnosed with \u201cListeria monocytogenes meningitis complicated by an acute exacerbation of chronic obstructive pulmonary disease\u201d. Patients with pathogenic bacterial infections completed a 21-day course of ampicillin and sulbactam sodium and a 14-day course of gentamicin, resulting in a rapid improvement in clinical symptoms and biochemical parameters.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n This case underscores the critical role of mNGS in the aetiological diagnosis of central nervous system infections, especially when conventional methods are inconclusive. It highlights the need for a high index of suspicion for listeriosis in elderly patients with comorbidities presenting with unexplained fever and neurological decline.<\/jats:p>\n <\/jats:sec>","DOI":"10.3389\/fmedt.2026.1801483","type":"journal-article","created":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T05:37:57Z","timestamp":1781069877000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":0,"title":["Case Report: Listeria monocytogenes meningitis complicated by an acute exacerbation of chronic obstructive pulmonary disease: the key diagnostic role of metagenomic high-throughput sequencing"],"prefix":"10.3389","volume":"8","author":[{"given":"Yiwei","family":"Chen","sequence":"first","affiliation":[{"name":"School of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine","place":["Lanzhou, Gansu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dacheng","family":"Tian","sequence":"additional","affiliation":[{"name":"School of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine","place":["Lanzhou, Gansu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yixuan","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine","place":["Lanzhou, Gansu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhui","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, Gansu Provincial Hospital of Traditional Chinese Medicine","place":["Lanzhou, Gansu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaichun","family":"Liu","sequence":"additional","affiliation":[{"name":"Respiratory Department, Wuwei Liangzhou Hospital","place":["Wuwei, Gansu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanwen","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Integrated Traditional Chinese and Western Medicine, Gansu University of Chinese Medicine","place":["Lanzhou, Gansu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xingfang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pulmonology, Gansu Provincial Hospital of Traditional Chinese Medicine","place":["Lanzhou, Gansu, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"e0006019","DOI":"10.1128\/cmr.00060-19","article-title":"Human listeriosis","volume":"36","author":"Koopmans","year":"2023","journal-title":"Clin Microbiol 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