{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:20:26Z","timestamp":1789507226360,"version":"build-2803163510"},"reference-count":54,"publisher":"Frontiers Media SA","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC81330035, NSFC31470193"],"award-info":[{"award-number":["NSFC81330035, NSFC31470193"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2016.00716","type":"journal-article","created":{"date-parts":[[2016,5,12]],"date-time":"2016-05-12T02:57:34Z","timestamp":1463021854000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":36,"title":["A Putative Mitochondrial Iron Transporter MrsA in Aspergillus fumigatus Plays Important Roles in Azole-, Oxidative Stress Responses and Virulence"],"prefix":"10.3389","volume":"7","author":[{"given":"Nanbiao","family":"Long","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Qian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizhu","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ling","family":"Lu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2016,5,12]]},"reference":[{"issue":"439","key":"B1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00439","article-title":"Ergosterol biosynthesis in Aspergillus fumigatus: its relevance as an antifungal target and role in antifungal drug resistance.","volume":"3","author":"Alcazar-Fuoli","year":"2012","journal-title":"Front. Microbiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.steroids.2007.11.005","article-title":"Ergosterol biosynthesis pathway in Aspergillus fumigatus.","volume":"73","author":"Alcazar-Fuoli","year":"2008","journal-title":"Steroids"},{"key":"B3","doi-asserted-by":"publisher","first-page":"350","DOI":"10.1016\/j.celrep.2012.12.021","article-title":"Fungicidal drugs induce a common oxidative-damage cellular death pathway.","volume":"3","author":"Belenky","year":"2013","journal-title":"Cell Rep."},{"key":"B4","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1042\/BJ20140107","article-title":"Three conserved histidine residues contribute to mitochondrial iron transport through mitoferrins.","volume":"460","author":"Brazzolotto","year":"2014","journal-title":"Biochem. J."},{"key":"B5","doi-asserted-by":"publisher","first-page":"847","DOI":"10.1128\/AAC.47.3.847-853.2003","article-title":"Relationships between respiration and susceptibility to azole antifungals in Candida glabrata.","volume":"47","author":"Brun","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"B6","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.fgb.2015.04.007","article-title":"The G beta-like protein CpcB is required for hyphal growth, conidiophore morphology and pathogenicity in Aspergillus fumigatus.","volume":"81","author":"Cai","year":"2015","journal-title":"Fungal Genet. Biol."},{"key":"B7","doi-asserted-by":"publisher","first-page":"118","DOI":"10.1016\/j.chom.2011.07.005","article-title":"An iron homeostasis regulatory circuit with reciprocal roles in Candida albicans commensalism and pathogenesis.","volume":"10","author":"Chen","year":"2011","journal-title":"Cell Host Microbe"},{"key":"B8","doi-asserted-by":"publisher","first-page":"1459","DOI":"10.1016\/j.bbamcr.2012.01.003","article-title":"Cellular and mitochondrial iron homeostasis in vertebrates.","volume":"1823","author":"Chen","year":"2012","journal-title":"Biochim. Biophys. Acta"},{"key":"B9","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1128\/AAC.40.2.419","article-title":"Correlation between rhodamine 123 accumulation and azole sensitivity in Candida species: possible role for drug e\ufb04ux in drug resistance.","volume":"40","author":"Clark","year":"1996","journal-title":"Antimicrob. Agents Chemother."},{"key":"B10","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1046\/j.1365-2958.2003.03586.x","article-title":"The siderophore system is essential for viability of Aspergillus nidulans: functional analysis of two genes encoding l-ornithine N 5-monooxygenase (sidA) and a non-ribosomal peptide synthetase (sidC).","volume":"49","author":"Eisendle","year":"2003","journal-title":"Mol. Microbiol."},{"key":"B11","doi-asserted-by":"publisher","first-page":"24475","DOI":"10.1074\/jbc.M111789200","article-title":"Deletion of the mitochondrial carrier genes MRS3 and MRS4 suppresses mitochondrial iron accumulation in a yeast frataxin-deficient strain.","volume":"277","author":"Foury","year":"2002","journal-title":"J. Biol. Chem."},{"key":"B12","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1042\/BJ20130144","article-title":"The mitochondrial carrier Rim2 co-imports pyrimidine nucleotides and iron.","volume":"455","author":"Froschauer","year":"2013","journal-title":"Biochem. J."},{"key":"B13","doi-asserted-by":"publisher","first-page":"2261","DOI":"10.15252\/embj.201489468","article-title":"The Janus transcription factor HapX controls fungal adaptation to both iron starvation and iron excess.","volume":"33","author":"Gsaller","year":"2014","journal-title":"EMBO J."},{"issue":"28","key":"B14","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2012.00028","article-title":"Iron - a key nexus in the virulence of Aspergillus fumigatus.","volume":"3","author":"Haas","year":"2012","journal-title":"Front. Microbiol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1042\/bj2190001","article-title":"Oxygen toxicity, oxygen radicals, transition metals and disease.","volume":"219","author":"Halliwell","year":"1984","journal-title":"Biochem. J."},{"key":"B16","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/0014-5793(92)80911-Y","article-title":"Biologically relevant metal ion-dependent hydroxyl radical generation, An update.","volume":"307","author":"Halliwell","year":"1992","journal-title":"FEBS Lett."},{"key":"B17","doi-asserted-by":"publisher","first-page":"5493","DOI":"10.1128\/IAI.73.9.5493-5503.2005","article-title":"The Aspergillus fumigatus siderophore biosynthetic gene sidA, encoding L-ornithine N5-oxygenase, is required for virulence.","volume":"73","author":"Hissen","year":"2005","journal-title":"Infect. Immun."},{"key":"B18","doi-asserted-by":"publisher","first-page":"62","DOI":"10.1016\/j.fgb.2013.11.003","article-title":"Deletion of the putative stretch-activated ion channel Mid1 is hypervirulent in Aspergillus fumigatus.","volume":"62","author":"Jiang","year":"2014","journal-title":"Fungal Genet. Biol."},{"issue":"e1001209","key":"B19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001209","article-title":"HapX positively and negatively regulates the transcriptional response to iron deprivation in Cryptococcus neoformans.","volume":"6","author":"Jung","year":"2010","journal-title":"PLoS Pathog."},{"issue":"e410","key":"B20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040410","article-title":"Iron regulation of the major virulence factors in the AIDS-associated pathogen Cryptococcus neoformans.","volume":"4","author":"Jung","year":"2006","journal-title":"PLoS Biol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/ics.12287","article-title":"Oxidative stress - a key emerging impact factor in health, ageing, lifestyle and aesthetics.","volume":"37","author":"Kandola","year":"2015","journal-title":"Int. J. Cosmetic Sci."},{"key":"B22","doi-asserted-by":"publisher","first-page":"3113","DOI":"10.1128\/AAC.46.10.3113-3117.2002","article-title":"Endogenous reactive oxygen species is an important mediator of miconazole antifungal effect.","volume":"46","author":"Kobayashi","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"B23","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1159\/000358901","article-title":"Oxidative stress, mitochondrial dysfunction and the mitochondria theory of aging.","volume":"39","author":"Kong","year":"2014","journal-title":"Interdiscip. Top. Gerontol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1007\/s00216-015-9197-x","article-title":"Measuring internal azole and pyrethroid pesticide concentrations in Daphnia magna using QuEChERS and GC-ECD-method development with a focus on matrix effects.","volume":"408","author":"Kretschmann","year":"2016","journal-title":"Anal. Bioanal. Chem."},{"key":"B25","doi-asserted-by":"publisher","first-page":"33653","DOI":"10.1074\/jbc.M403146200","article-title":"A mitochondrial-vacuolar signaling pathway in yeast that affects iron and copper metabolism.","volume":"279","author":"Li","year":"2004","journal-title":"J. Biol. Chem."},{"key":"B26","doi-asserted-by":"publisher","first-page":"10232","DOI":"10.1074\/jbc.M109.096859","article-title":"Genetic dissection of a mitochondria-vacuole signaling pathway in yeast reveals a link between chronic oxidative stress and vacuolar iron transport.","volume":"285","author":"Li","year":"2010","journal-title":"J. Biol. Chem."},{"issue":"e99805","key":"B27","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099805","article-title":"Vitamin D deficiency causes defective resistance to Aspergillus fumigatus in mice via aggravated and sustained inflammation.","volume":"9","author":"Li","year":"2014","journal-title":"PLoS ONE"},{"key":"B28","doi-asserted-by":"publisher","first-page":"3851","DOI":"10.1074\/jbc.M110.190959","article-title":"Genetic and biochemical analysis of high iron toxicity in yeast: iron toxicity is due to the accumulation of cytosolic iron and occurs under both aerobic and anaerobic conditions.","volume":"286","author":"Lin","year":"2011","journal-title":"J. Biol. Chem."},{"key":"B29","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1016\/j.fgb.2014.12.005","article-title":"Calcium signaling mediates antifungal activity of triazole drugs in the Aspergilli.","volume":"81","author":"Liu","year":"2015","journal-title":"Fungal Genet. Biol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"3805","DOI":"10.1105\/tpc.112.098624","article-title":"HapX-mediated iron homeostasis is essential for rhizosphere competence and virulence of the soilborne pathogen Fusarium oxysporum.","volume":"24","author":"Lopez-Berges","year":"2012","journal-title":"Plant Cell"},{"key":"B31","doi-asserted-by":"publisher","first-page":"3335","DOI":"10.1099\/00221287-147-12-3335","article-title":"Assessment of mitochondrial membrane potential in yeast cell populations by flow cytometry.","volume":"147","author":"Ludovico","year":"2001","journal-title":"Microbiology"},{"issue":"e1005340","key":"B32","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005340","article-title":"A trypanosomatid iron transporter that regulates mitochondrial function is required for Leishmania amazonensis virulence.","volume":"12","author":"Mittra","year":"2016","journal-title":"PLoS Pathog."},{"key":"B33","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1016\/j.mib.2013.07.012","article-title":"The crucial role of iron uptake in Aspergillus fumigatus virulence.","volume":"16","author":"Moore","year":"2013","journal-title":"Curr. Opin. Microbiol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"40612","DOI":"10.1074\/jbc.M307847200","article-title":"A specific role of the yeast mitochondrial carriers Mrs3\/4p in mitochondrial iron acquisition under iron-limiting conditions.","volume":"278","author":"Muhlenhoff","year":"2003","journal-title":"J. Biol. Chem."},{"key":"B35","doi-asserted-by":"publisher","first-page":"4333","DOI":"10.1128\/IAI.71.8.4333-4340.2003","article-title":"Mechanism of fluconazole resistance in Candida albicans biofilms: phase-specific role of e\ufb04ux pumps and membrane sterols.","volume":"71","author":"Mukherjee","year":"2003","journal-title":"Infect. Immun."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1077","DOI":"10.1046\/j.1365-2958.2001.02586.x","article-title":"SREA is involved in regulation of siderophore biosynthesis, utilization and uptake in Aspergillus nidulans.","volume":"41","author":"Oberegger","year":"2001","journal-title":"Mol. Microbiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1128\/MCB.01685-08","article-title":"Regulation of mitochondrial iron import through differential turnover of mitoferrin 1 and Mitoferrin 2.","volume":"29","author":"Paradkar","year":"2009","journal-title":"Mol. Cell. Biol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1333","DOI":"10.1128\/EC.00159-12","article-title":"Structural requirements for the activity of the MirB ferrisiderophore transporter of Aspergillus fumigatus.","volume":"11","author":"Raymond-Bouchard","year":"2012","journal-title":"Eukaryot. Cell"},{"issue":"e1001124","key":"B39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001124","article-title":"HapX-mediated adaption to iron starvation is crucial for virulence of Aspergillus fumigatus.","volume":"6","author":"Schrettl","year":"2010","journal-title":"PLoS Pathog."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1213","DOI":"10.1084\/jem.20041242","article-title":"Siderophore biosynthesis but not reductive iron assimilation is essential for Aspergillus fumigatus virulence.","volume":"200","author":"Schrettl","year":"2004","journal-title":"J. Exp. Med."},{"key":"B41","doi-asserted-by":"publisher","first-page":"400","DOI":"10.1016\/j.mib.2011.06.002","article-title":"Iron homeostasis\u2013Achilles\u2019 heel of Aspergillus fumigatus?","volume":"14","author":"Schrettl","year":"2011","journal-title":"Curr. Opin. Microbiol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1111\/j.1365-2958.2008.06376.x","article-title":"SreA-mediated iron regulation in Aspergillus fumigatus.","volume":"70","author":"Schrettl","year":"2008","journal-title":"Mol. Microbiol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"1376","DOI":"10.1128\/EC.05184-11","article-title":"Mitochondria and fungal pathogenesis: drug tolerance, virulence, and potential for antifungal therapy.","volume":"10","author":"Shingu-Vazquez","year":"2011","journal-title":"Eukaryot. Cell"},{"issue":"e1919-15","key":"B44","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01919-15","article-title":"The Aspergillus fumigatus damage resistance protein family coordinately regulates ergosterol biosynthesis and azole susceptibility.","volume":"7","author":"Song","year":"2015","journal-title":"mBio"},{"key":"B45","doi-asserted-by":"publisher","first-page":"3111","DOI":"10.1038\/nprot.2006.405","article-title":"Fusion PCR and gene targeting in Aspergillus nidulans.","volume":"1","author":"Szewczyk","year":"2006","journal-title":"Nat. Protoc."},{"key":"B46","doi-asserted-by":"publisher","first-page":"5580","DOI":"10.1128\/AAC.00889-13","article-title":"Mitochondria influence CDR1 e\ufb04ux pump activity, Hog1-mediated oxidative stress pathway, iron homeostasis, and ergosterol levels in Candida albicans.","volume":"57","author":"Thomas","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"issue":"150223","key":"B47","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.150223","article-title":"Overlap of copper and iron uptake systems in mitochondria in Saccharomyces cerevisiae.","volume":"6","author":"Vest","year":"2016","journal-title":"Open Biol."},{"key":"B48","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1007\/s12275-015-5014-7","article-title":"The molecular mechanism of azole resistance in Aspergillus fumigatus: from bedside to bench and back.","volume":"53","author":"Wei","year":"2015","journal-title":"J. Microbiol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"844","DOI":"10.1111\/j.1567-1364.2012.00835.x","article-title":"Identification and functional characterization of mitochondrial carrier Mrs4 in Candida albicans.","volume":"12","author":"Xu","year":"2012","journal-title":"FEMS Yeast Res."},{"key":"B50","doi-asserted-by":"publisher","first-page":"629","DOI":"10.1016\/j.bbamcr.2013.12.009","article-title":"Cellular iron homeostasis mediated by the Mrs4-Ccc1-Smf3 pathway is essential for mitochondrial function, morphogenesis and virulence in Candida albicans.","volume":"1843","author":"Xu","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"B51","doi-asserted-by":"publisher","first-page":"E497","DOI":"10.1073\/pnas.1106399108","article-title":"Mevalonate governs interdependency of ergosterol and siderophore biosyntheses in the fungal pathogen Aspergillus fumigatus.","volume":"109","author":"Yasmin","year":"2012","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B52","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1042\/BJ20111036","article-title":"Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria.","volume":"440","author":"Yoon","year":"2011","journal-title":"Biochem. J."},{"key":"B53","doi-asserted-by":"publisher","first-page":"250","DOI":"10.1016\/j.fgb.2015.03.001","article-title":"The newly nonsporulated characterization of an Aspergillus fumigatus isolate from an immunocompetent patient and its clinic indication.","volume":"81","author":"Zhang","year":"2015","journal-title":"Fungal Genet. Biol."},{"key":"B54","first-page":"652","article-title":"Rapid determination of thiabendazole and carbendazim in concentrated fruit juices by ultra-high performance liquid chromatography-tandem mass spectrometry.","volume":"33","author":"Zheng","year":"2015","journal-title":"Se Pu"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"deposited":{"date-parts":[[2019,9,7]],"date-time":"2019-09-07T11:16:54Z","timestamp":1567855014000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/Article\/10.3389\/fmicb.2016.00716\/abstract"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,12]]},"references-count":54,"URL":"https:\/\/doi.org\/10.3389\/fmicb.2016.00716","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,12]]}}}