{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T10:47:28Z","timestamp":1789555648227,"version":"build-2803163510"},"reference-count":51,"publisher":"Frontiers Media SA","funder":[{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["(PS194\/2009C) and (UM.C\/HIR\/MOHE\/MED\/16)"],"award-info":[{"award-number":["(PS194\/2009C) and (UM.C\/HIR\/MOHE\/MED\/16)"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2016.00808","type":"journal-article","created":{"date-parts":[[2016,5,27]],"date-time":"2016-05-27T16:00:58Z","timestamp":1464364858000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":44,"title":["Induction of Protective Immunity against Toxoplasmosis in BALB\/c Mice Vaccinated with Toxoplasma gondii Rhoptry-1"],"prefix":"10.3389","volume":"7","author":[{"given":"Parthasarathy","family":"Sonaimuthu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao T.","family":"Ching","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mun Y.","family":"Fong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramaswamy","family":"Kalyanasundaram","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yee L.","family":"Lau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2016,5,27]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1038\/383787a0","article-title":"Functional diversity of helper T lymphocytes.","volume":"383","author":"Abbas","year":"1996","journal-title":"Nature"},{"key":"B2","doi-asserted-by":"crossref","first-page":"2082","DOI":"10.1128\/IAI.61.5.2082-2088.1993","article-title":"Oral immunization with Toxoplasma gondii antigens in association with cholera toxin induces enhanced protective and cell-mediated immunity in C57BL\/6 mice.","volume":"61","author":"Bourguin","year":"1993","journal-title":"Infect. Immun."},{"key":"B3","first-page":"289","article-title":"Protozoan infections (Toxoplasma gondii, Neospora caninum and Sarcocystis spp.) in sheep and goats: recent advances.","volume":"29","author":"Buxton","year":"1998","journal-title":"Vet. Res."},{"key":"B4","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1136\/vr.129.5.89","article-title":"Vaccination of sheep with a live incomplete strain (S48) of Toxoplasma gondii and their immunity to challenge when pregnant.","volume":"129","author":"Buxton","year":"1991","journal-title":"Vet. Rec."},{"key":"B5","doi-asserted-by":"publisher","first-page":"1320","DOI":"10.1128\/EC.3.5.1320-1330.2004","article-title":"The Toxoplasma gondii rhoptry protein ROP4 is secreted into the parasitophorous vacuole and becomes phosphorylated in infected cells.","volume":"3","author":"Carey","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"B6","first-page":"837","article-title":"Construction of a recombinant plasmid harbouring the rhoptry protein 1 gene of Toxoplasma gondii and preliminary observations on DNA immunity.","volume":"114","author":"Chen","year":"2001","journal-title":"Chin. Med. J. (Engl.)"},{"key":"B7","first-page":"1561","article-title":"Induction of immune responses in mice by vaccination with Liposome-entrapped DNA complexes encoding Toxoplasma gondii SAG1 and ROP1 genes.","volume":"116","author":"Chen","year":"2003","journal-title":"Chin. Med. J. (Engl.)"},{"issue":"609","key":"B8","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00609","article-title":"Evaluation of immunoprotection conferred by the subunit vaccines of GRA2 and GRA5 against acute toxoplasmosis in BALB\/c mice.","volume":"7","author":"Ching","year":"2016","journal-title":"Front. Microbiol."},{"key":"B9","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1128\/CMR.11.4.569","article-title":"Regulation and function of T-cell-mediated immunity during Toxoplasma gondii infection.","volume":"11","author":"Denkers","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"B10","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.micinf.2003.11.009","article-title":"The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection.","volume":"6","author":"Di Cristina","year":"2004","journal-title":"Microbes Infect."},{"issue":"632424","key":"B11","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/632424","article-title":"Toxoplasma rhoptries: unique secretory organelles and source of promising vaccine proteins for immunoprevention of toxoplasmosis.","volume":"2008","author":"Dlugonska","year":"2008","journal-title":"J. Biomed. Biotechnol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1016\/j.exppara.2012.02.026","article-title":"Toxoplasma gondii: the vaccine potential of three trivalent antigen-cocktails composed of recombinant ROP2, ROP4, GRA4 and SAG1 proteins against chronic toxoplasmosis in BALB\/c mice.","volume":"131","author":"Dziadek","year":"2012","journal-title":"Exp. Parasitol."},{"key":"B13","doi-asserted-by":"crossref","first-page":"5449","DOI":"10.4049\/jimmunol.162.9.5449","article-title":"Augmentation of the CD8+ T cell response by IFN-gamma in IL-12-deficient mice during Toxoplasma gondii infection.","volume":"162","author":"Ely","year":"1999","journal-title":"J. Immunol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"8735","DOI":"10.5897\/AJB10.1860","article-title":"Induction of protective immunity against toxoplasmosis in mice by immunization with a plasmid encoding Toxoplama gondii ROP1 gene.","volume":"11","author":"Eslamirad","year":"2012","journal-title":"Afr. J. Biotechnol."},{"key":"B15","first-page":"71","article-title":"Immune response to Toxoplasma gondii.","volume":"40","author":"Filisetti","year":"2004","journal-title":"Ann. Ist. Super. Sanita"},{"key":"B16","doi-asserted-by":"crossref","first-page":"286","DOI":"10.4049\/jimmunol.146.1.286","article-title":"Synergistic role of CD4+ and CD8+ T lymphocytes in IFN-gamma production and protective immunity induced by an attenuated Toxoplasma gondii vaccine.","volume":"146","author":"Gazzinelli","year":"1991","journal-title":"J. Immunol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"4301","DOI":"10.1016\/j.vaccine.2007.02.057","article-title":"Evaluation of protective effect of recombinant dense granule antigens GRA2 and GRA6 formulated in monophosphoryl lipid A (MPL) adjuvant against Toxoplasma chronic infection in mice.","volume":"25","author":"Golkar","year":"2007","journal-title":"Vaccine"},{"key":"B18","first-page":"317","article-title":"Immunity induced by DNA vaccine of plasmid encoding the rhoptry protein 1 gene combined with the genetic adjuvant of pcIFN-gamma against Toxoplasma gondii in mice.","volume":"114","author":"Guo","year":"2001","journal-title":"Chin. Med. J. (Engl.)"},{"key":"B19","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1146\/annurev.immunol.18.1.927","article-title":"DNA vaccines: immunology, application, and optimization*.","volume":"18","author":"Gurunathan","year":"2000","journal-title":"Annu. Rev. Immunol."},{"key":"B20","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/S0968-0004(00)89045-3","article-title":"Purification of his-tag fusion proteins from Escherichia coli.","volume":"20","author":"Hengen","year":"1995","journal-title":"Trends Biochem. Sci."},{"key":"B21","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/j.vetpar.2011.06.010","article-title":"Modulation of immune response to Toxoplasma gondii in sheep by immunization with a DNA vaccine encoding ROP1 antigen as a fusion protein with ovine CD154.","volume":"183","author":"Hiszczynska-Sawicka","year":"2011a","journal-title":"Vet. Parasitol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.vetpar.2010.11.047","article-title":"Evaluation of immune responses in sheep induced by DNA immunization with genes encoding GRA1, GRA4, GRA6 and GRA7 antigens of Toxoplasma gondii.","volume":"177","author":"Hiszczynska-Sawicka","year":"2011b","journal-title":"Vet. Parasitol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"6222","DOI":"10.1128\/IAI.71.11.6222-6228.2003","article-title":"The MIC3 gene of Toxoplasma gondii is a novel potent vaccine candidate against toxoplasmosis.","volume":"71","author":"Ismael","year":"2003","journal-title":"Infect. Immun."},{"key":"B24","article-title":"\u201cThe destruction of antibody-coated pathogens via Fc receptors,\u201d in","author":"Janeway","year":"2001","journal-title":"Immunobiology: The Immune System in Health and Disease"},{"key":"B25","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1016\/j.vaccine.2007.11.058","article-title":"An enhanced GRA1-GRA7 cocktail DNA vaccine primes anti-Toxoplasma immune responses in pigs.","volume":"26","author":"Jongert","year":"2008","journal-title":"Vaccine"},{"key":"B26","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1590\/S0074-02762009000200019","article-title":"Vaccines against Toxoplasma gondii: challenges and opportunities.","volume":"104","author":"Jongert","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"B27","doi-asserted-by":"publisher","first-page":"318","DOI":"10.1016\/j.exppara.2010.05.008","article-title":"Regulation of CD8+ T cell responses to infection with parasitic protozoa.","volume":"126","author":"Jordan","year":"2010","journal-title":"Exp. Parasitol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1080\/01621459.1958.10501452","article-title":"Nonparametric estimation from incomplete observations.","volume":"53","author":"Kaplan","year":"1958","journal-title":"J. Am. Stat. Associ.n."},{"key":"B29","doi-asserted-by":"crossref","first-page":"3501","DOI":"10.4049\/jimmunol.147.10.3501","article-title":"A purified parasite antigen (p30) mediates CD8+ T cell immunity against fatal Toxoplasma gondii infection in mice.","volume":"147","author":"Khan","year":"1991","journal-title":"J. Immunol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/j.ijpara.2003.12.009","article-title":"Toxoplasma gondii: the model apicomplexan.","volume":"34","author":"Kim","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1007\/s00436-011-2315-6","article-title":"Immunogenic characterization of the chimeric surface antigen 1 and 2 (SAG1\/2) of Toxoplasma gondii expressed in the yeast Pichia pastoris.","volume":"109","author":"Lau","year":"2011","journal-title":"Parasitol. Res."},{"key":"B32","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1007\/s004360000296","article-title":"Genetic immunization with plasmid DNA coding for the ROP2 protein of Toxoplasma gondii.","volume":"87","author":"Leyva","year":"2001","journal-title":"Parasitol. Res."},{"key":"B33","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1111\/j.1365-3024.2010.01228.x","article-title":"Immunological response of sheep to injections of plasmids encoding Toxoplasma gondii SAG1 and ROP1 genes.","volume":"32","author":"Li","year":"2010","journal-title":"Parasite Immunol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1305","DOI":"10.4161\/hv.21006","article-title":"Vaccines against Toxoplasma gondii: status, challenges and future directions.","volume":"8","author":"Liu","year":"2012","journal-title":"Hum. Vaccin. Immunother."},{"key":"B35","doi-asserted-by":"publisher","first-page":"1244","DOI":"10.1016\/j.micinf.2005.11.013","article-title":"Immunization with MIC1 and MIC4 induces protective immunity against Toxoplasma gondii.","volume":"8","author":"Lourenco","year":"2006","journal-title":"Microbes Infect."},{"key":"B36","doi-asserted-by":"crossref","first-page":"704","DOI":"10.1128\/CDLI.11.4.704-710.2004","article-title":"Recombinant GRA4 or ROP2 protein combined with alum or the gra4 gene provides partial protection in chronic murine models of toxoplasmosis.","volume":"11","author":"Martin","year":"2004","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"4489","DOI":"10.1016\/j.vaccine.2005.04.025","article-title":"Evaluation of protective effect of DNA vaccination with genes encoding antigens GRA4 and SAG1 associated with GM-CSF plasmid, against acute, chronical and congenital toxoplasmosis in mice.","volume":"23","author":"Mevelec","year":"2005","journal-title":"Vaccine"},{"key":"B38","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1292\/jvms.63.433","article-title":"Modified protection against Toxoplasma gondii lethal infection and brain cyst formation by vaccination with SAG2 and SRS1.","volume":"63","author":"Mishima","year":"2001","journal-title":"J. Vet. Med. Sci."},{"key":"B39","doi-asserted-by":"publisher","first-page":"883","DOI":"10.4269\/ajtmh.12-0727","article-title":"Protective immune response in BALB\/c mice induced by DNA vaccine of the ROP8 gene of Toxoplasma gondii.","volume":"88","author":"Parthasarathy","year":"2013","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"B40","doi-asserted-by":"publisher","first-page":"6570","DOI":"10.1016\/j.vaccine.2009.08.043","article-title":"Toxoplasma gondii microneme protein 6 (MIC6) is a potential vaccine candidate against toxoplasmosis in mice.","volume":"27","author":"Peng","year":"2009","journal-title":"Vaccine"},{"key":"B41","doi-asserted-by":"publisher","first-page":"666","DOI":"10.1128\/CVI.05385-11","article-title":"Induction of protective immune responses by a multiantigenic DNA vaccine encoding GRA7 and ROP1 of Toxoplasma gondii.","volume":"19","author":"Quan","year":"2012","journal-title":"Clin. Vacci. Immunol."},{"key":"B42","first-page":"549","article-title":"DNA vaccines: basic mechanism and immune responses (Review).","volume":"4","author":"Robinson","year":"1999","journal-title":"Int. J. Mol. Med."},{"key":"B43","doi-asserted-by":"publisher","first-page":"227","DOI":"10.1146\/annurev.iy.12.040194.001303","article-title":"Lymphokine production by human T cells in disease states.","volume":"12","author":"Romagnani","year":"1994","journal-title":"Annu. Rev. Immunol."},{"key":"B44","doi-asserted-by":"publisher","first-page":"1780","DOI":"10.1126\/science.1133690","article-title":"Polymorphic secreted kinases are key virulence factors in toxoplasmosis.","volume":"314","author":"Saeij","year":"2006","journal-title":"Science"},{"issue":"213","key":"B45","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-4-213","article-title":"DNA vaccination with a gene encoding Toxoplasma gondii GRA6 induces partial protection against toxoplasmosis in BALB\/c mice.","volume":"4","author":"Sun","year":"2011","journal-title":"Parasit. Vectors"},{"key":"B46","doi-asserted-by":"crossref","first-page":"1954","DOI":"10.4049\/jimmunol.144.5.1954","article-title":"The effect of anti-IFN-gamma antibody on the protective effect of Lyt-2+ immune T cells against toxoplasmosis in mice.","volume":"144","author":"Suzuki","year":"1990","journal-title":"J. Immunol."},{"key":"B47","doi-asserted-by":"publisher","first-page":"934","DOI":"10.1016\/0042-6822(91)90576-W","article-title":"Identification and characterization of a new base substitution in the vaccine strain of Sabin 3 poliovirus.","volume":"185","author":"Weeks-Levy","year":"1991","journal-title":"Virology"},{"key":"B48","doi-asserted-by":"publisher","first-page":"684","DOI":"10.1128\/CVI.05578-11","article-title":"Vaccination with a DNA vaccine coding for perforin-like protein 1 and MIC6 induces significant protective immunity against Toxoplasma gondii.","volume":"19","author":"Yan","year":"2012","journal-title":"Clin. Vacci. Immunol."},{"key":"B49","doi-asserted-by":"publisher","first-page":"58","DOI":"10.1007\/s00436-003-0992-5","article-title":"Protective immunity against Toxoplasma gondii in mice induced by a chimeric protein rSAG1\/2.","volume":"92","author":"Yang","year":"2004","journal-title":"Parasitol. Res."},{"key":"B50","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1128\/CVI.00312-10","article-title":"Protective immunity induced by Toxoplasma gondii rhoptry protein 16 against toxoplasmosis in mice.","volume":"18","author":"Yuan","year":"2011a","journal-title":"Clin. Vacci. Immunol."},{"key":"B51","doi-asserted-by":"publisher","first-page":"6614","DOI":"10.1016\/j.vaccine.2011.06.110","article-title":"Protective effect against toxoplasmosis in mice induced by DNA immunization with gene encoding Toxoplasma gondii ROP18.","volume":"29","author":"Yuan","year":"2011b","journal-title":"Vaccine"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"deposited":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T17:16:53Z","timestamp":1692379013000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/Article\/10.3389\/fmicb.2016.00808\/abstract"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,27]]},"references-count":51,"URL":"https:\/\/doi.org\/10.3389\/fmicb.2016.00808","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,27]]}}}