{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T21:30:47Z","timestamp":1792445447549,"version":"4.5.0"},"reference-count":70,"publisher":"Frontiers Media SA","funder":[{"DOI":"10.13039\/501100001054","name":"Meat and Livestock Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000933","name":"Department of Agriculture, Fisheries and Forestry, Australian Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000933","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2016.01122","type":"journal-article","created":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T03:05:02Z","timestamp":1468897502000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":121,"title":["Methane Inhibition Alters the Microbial Community, Hydrogen Flow, and Fermentation Response in the Rumen of Cattle"],"prefix":"10.3389","volume":"7","author":[{"given":"Gonzalo","family":"Martinez-Fernandez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart E.","family":"Denman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Mitsumori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher S.","family":"McSweeney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2016,7,19]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"2027","DOI":"10.3168\/jds.2011-4831","article-title":"Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats.","volume":"95","author":"Abecia","year":"2012","journal-title":"J. Dairy Sci."},{"key":"B2","first-page":"576","article-title":"Formate metabolism by ruminal microorganisms in relation to methanogenesis.","volume":"69","author":"Asanuma","year":"1998","journal-title":"Anim. Sci. Technol."},{"key":"B3","author":"Association of Official Analytical Chemists [AOAC]","year":"2005","journal-title":"Official Methods of Analysis"},{"key":"B4","doi-asserted-by":"crossref","first-page":"1346","DOI":"10.1128\/JB.85.6.1346-1349.1963","article-title":"Conversion of glucose-C14 to propionate by the rumen microbiota.","volume":"85","author":"Baldwin","year":"1963","journal-title":"J. Bacteriol."},{"key":"B5","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1128\/AEM.42.6.1103-1110.1981","article-title":"Cellulose fermentation by a rumen anaerobic fungus in both the absence and the presence of rumen methanogens.","volume":"42","author":"Bauchop","year":"1981","journal-title":"Appl. Environ. Microbiol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1007\/BF00173998","article-title":"Formate production and utilisation by methanogens and by sludge consortia: interference with the concept of interspecies formate transfer.","volume":"40","author":"Bleicher","year":"1994","journal-title":"Appl. Microbiol. Biot."},{"key":"B7","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.1128\/AEM.55.7.1735-1741.1989","article-title":"Diffusion of the interspecies electron carriers H2 and formate in methanogenic ecosystems and its implications in the measurement of Km for H2 or formate uptake.","volume":"55","author":"Boone","year":"1989","journal-title":"Appl. Environ. Microbiol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"7523","DOI":"10.1021\/ac201610x","article-title":"Comprehensive profiling and quantitation of amine group containing metabolites.","volume":"83","author":"Boughton","year":"2011","journal-title":"Anal. Chem."},{"key":"B9","first-page":"139","article-title":"\u201cAnaerobic fungal populations,\u201d in Methods in Gut Microbial Ecology for Ruminants,","author":"Brookman","year":"2005"},{"key":"B10","doi-asserted-by":"publisher","first-page":"335","DOI":"10.1038\/nmeth.f.303","article-title":"QIIME allows analysis of high-throughput community sequencing data.","volume":"7","author":"Caporaso","year":"2010","journal-title":"Nat. Methods"},{"key":"B11","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1093\/clinchem\/8.2.130","article-title":"Modified reagents for determination of urea and ammonia.","volume":"8","author":"Chaney","year":"1962","journal-title":"Clin. Chem."},{"key":"B12","first-page":"5","article-title":"The ade4 package. I. One-table methods.","volume":"4","author":"Chessel","year":"2004","journal-title":"R. News"},{"issue":"1087","key":"B13","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01087","article-title":"Metagenomic analysis of the rumen microbial community following inhibition of methane formation by a halogenated methane analog.","volume":"6","author":"Denman","year":"2015","journal-title":"Front. Microbiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"572","DOI":"10.1111\/j.1574-6941.2006.00190.x","article-title":"Development of a real-time PCR assay for monitoring anaerobic fungal and cellulolytic bacterial populations within the rumen.","volume":"58","author":"Denman","year":"2006","journal-title":"FEMS Microbiol. Ecol."},{"key":"B15","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1111\/j.1574-6941.2007.00394.x","article-title":"Quantitation and diversity analysis of ruminal methanogenic populations in response to the antimethanogenic compound bromochloromethane.","volume":"62","author":"Denman","year":"2007","journal-title":"FEMS Microbiol. Ecol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jchromb.2015.07.002","article-title":"Quantitative profiling of polar primary metabolites of two chickpea cultivars with contrasting responses to salinity.","volume":"1000","author":"Dias","year":"2015","journal-title":"J. Chromatogr. B"},{"key":"B17","doi-asserted-by":"publisher","first-page":"491","DOI":"10.4141\/A99-024","article-title":"Effects of exogenous fibrolytic enzymes, \u03b1-bromoethanesulfonate and monensin on fermentation in a rumen simulation (RUSITEC) system.","volume":"79","author":"Dong","year":"1999","journal-title":"Can. J. Anim. Sci."},{"key":"B18","doi-asserted-by":"publisher","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST.","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"B19","doi-asserted-by":"publisher","first-page":"6391","DOI":"10.1128\/AEM.00181-07","article-title":"Establishment and development of ruminal hydrogenotrophs in methanogen-free lambs.","volume":"73","author":"Fonty","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"issue":"314","key":"B20","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-014-0314-3","article-title":"Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach.","volume":"14","author":"Gagen","year":"2014","journal-title":"BMC Microbiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"1484","DOI":"10.1017\/S0007114508076198","article-title":"Inhibition of methanogens by bromochloromethane: effects on microbial communities and rumen fermentation using batch and continuous fermentations.","volume":"101","author":"Goel","year":"2009","journal-title":"Br. J. Nutr."},{"key":"B22","doi-asserted-by":"publisher","first-page":"133","DOI":"10.1039\/b103714p","article-title":"Elucidation of methanogenic coenzyme biosyntheses: from spectroscopy to genomics.","volume":"19","author":"Graham","year":"2002","journal-title":"Nat. Prod. Rep."},{"key":"B23","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1128\/JB.135.3.851-857.1978","article-title":"ATP activation and properties of the methyl coenzyme M reductase system in Methanobacterium thermoautotrophicum.","volume":"135","author":"Gunsalus","year":"1978","journal-title":"J. Bacteriol."},{"key":"B24","doi-asserted-by":"publisher","DOI":"10.1071\/AN15219","article-title":"The effects of feeding 3-nitrooxypropanol at two doses on milk production, rumen fermentation, plasma metabolites, nutrient digestibility, and methane emissions in lactating Holstein cows.","author":"Haisan","year":"2016","journal-title":"Anim. Prod. Sci."},{"key":"B25","doi-asserted-by":"crossref","first-page":"1368","DOI":"10.1128\/AEM.50.6.1368-1374.1985","article-title":"Effect of reducing-equivalent disposal and NADH\/NAD on deamination of amino acids by intact rumen microorganisms and their cell extracts.","volume":"50","author":"Hino","year":"1985","journal-title":"Appl. Environ. Microbiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"5045","DOI":"10.2527\/jas.2013-6583","article-title":"Special Topics\u2014mitigation of methane and nitrous oxide emissions from animal operations: I. A review of enteric methane mitigation options.","volume":"91","author":"Hristov","year":"2013","journal-title":"J. Anim. Sci."},{"key":"B27","doi-asserted-by":"publisher","first-page":"10663","DOI":"10.1073\/pnas.1504124112","article-title":"An inhibitor persistently decreased enteric methane emission from dairy cows with no negative effect on milk production.","volume":"112","author":"Hristov","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B28","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1128\/JB.102.2.389-397.1970","article-title":"Formate as an intermediate in the bovine rumen fermentation.","volume":"102","author":"Hungate","year":"1970","journal-title":"J. Bacteriol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"1187","DOI":"10.5352\/JLS.2012.22.9.1187","article-title":"Effects of halogenated compounds on in vitro fermentation characteristics in the rumen and methane emissions.","volume":"22","author":"Hwang","year":"2012","journal-title":"J. Life Sci."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.anifeedsci.2010.07.002","article-title":"Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics.","volume":"160","author":"Janssen","year":"2010","journal-title":"Anim. Feed Sci. Technol."},{"key":"B31","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1071\/AR99004","article-title":"Ruminal acetogens and their potential to lower ruminant methane emissions.","volume":"50","author":"Joblin","year":"1999","journal-title":"Austr. J. Agr. Res."},{"key":"B32","doi-asserted-by":"crossref","first-page":"2483","DOI":"10.2527\/1995.7382483x","article-title":"Methane emissions from cattle.","volume":"73","author":"Johnson","year":"1995","journal-title":"J. Anim. Sci."},{"key":"B33","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1128\/jb.92.5.1321-1330.1966","article-title":"Enzymatic studies of pure cultures of rumen microorganisms.","volume":"92","author":"Joyner","year":"1966","journal-title":"J. Bacteriol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1016\/j.anifeedsci.2011.04.059","article-title":"Chloroform decreases rumen methanogenesis and methanogen populations without altering rumen function in cattle.","volume":"16","author":"Knight","year":"2011","journal-title":"Anim. Feed Sci. Technol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform.","volume":"79","author":"Kozich","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1071\/AN13381","article-title":"Interactions between microbial consortia in biofilms: a paradigm shift in rumen microbial ecology and enteric methane mitigation.","volume":"54","author":"Leng","year":"2014","journal-title":"Anim. Prod. Sci."},{"key":"B37","doi-asserted-by":"publisher","first-page":"494","DOI":"10.1007\/s00248-015-0641-4","article-title":"Amino acid and peptide utilization profiles of the fluoroacetate-degrading bacterium Synergistetes Strain MFA1 under varying conditions.","volume":"71","author":"Leong","year":"2016","journal-title":"Microb. Ecol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1099","DOI":"10.1017\/S1751731113000116","article-title":"Effects of nitrate adaptation by rumen inocula donors and substrate fiber proportion on in vitro nitrate disappearance, methanogenesis, and rumen fermentation acid.","volume":"7","author":"Lin","year":"2013","journal-title":"Animal"},{"key":"B39","doi-asserted-by":"publisher","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2-\u0394\u0394CT method.","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"issue":"550","key":"B40","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"B41","doi-asserted-by":"publisher","first-page":"3790","DOI":"10.3168\/jds.2013-7398","article-title":"Effects of ethyl-3-nitrooxy propionate and 3-nitrooxypropanol on ruminal fermentation, microbial abundance, and methane emissions in sheep.","volume":"97","author":"Martinez-Fernandez","year":"2014","journal-title":"J. Dairy Sci."},{"key":"B42","doi-asserted-by":"publisher","DOI":"10.1093\/femsec\/fiv079","article-title":"Response of the rumen archaeal and bacterial populations to anti-methanogenic organosulphur compounds in continuous-culture fermenters.","volume":"91","author":"Martinez-Fernandez","year":"2015","journal-title":"FEMS Microbiol. Ecol."},{"key":"B43","author":"May","year":"1995","journal-title":"A Delivery System for Agents. AU International Patent PCT\/AU95\/00733"},{"key":"B44","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1079\/BJN19730107","article-title":"Degradation of nucleic acids in the rumen.","volume":"29","author":"McAllan","year":"1973","journal-title":"Br. J. Nutr."},{"key":"B45","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1071\/EA07218","article-title":"Redirecting rumen fermentation to reduce methanogenesis.","volume":"48","author":"McAllister","year":"2008","journal-title":"Aust. J. Exp.Agr."},{"issue":"e91864","key":"B46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0091864","article-title":"Relationship between the rumen microbiome and residual feed intake-efficiency of Brahman bulls stocked on bermudagrass pastures.","volume":"9","author":"McCann","year":"2014","journal-title":"PLoS ONE"},{"key":"B47","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1038\/ismej.2011.139","article-title":"An improved greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea.","volume":"6","author":"McDonald","year":"2012","journal-title":"ISME J."},{"issue":"e61217","key":"B48","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217","article-title":"phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data.","volume":"8","author":"McMurdie","year":"2013","journal-title":"PLoS ONE"},{"key":"B49","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1017\/S0007114511005794","article-title":"Responses in digestion, rumen fermentation and microbial populations to inhibition of methane formation by a halogenated methane analogue.","volume":"108","author":"Mitsumori","year":"2012","journal-title":"Br. J. Nutr."},{"key":"B50","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1079\/BJN20051445","article-title":"Proprionate precursors and other metabolic intermediates as possible alternative electron acceptors to methanogenesis in ruminal fermentation in vitro.","volume":"94","author":"Newbold","year":"2005","journal-title":"Br. J. Nutr."},{"key":"B51","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1071\/AN12204","article-title":"Reducing in vitro rumen methanogenesis for two contrasting diets using a series of inclusion rates of different additives.","volume":"54","author":"O\u2019Brien","year":"2013","journal-title":"Anim. Prod. Sci."},{"key":"B52","doi-asserted-by":"publisher","first-page":"1429","DOI":"10.3168\/jds.S0022-0302(80)83100-6","article-title":"Effect of nicotinic acid on rumen fermentation in vitro and in vivo.","volume":"63","author":"Riddell","year":"1980","journal-title":"J. Dairy Sci."},{"issue":"e1005846","key":"B53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1005846","article-title":"Bovine host genetic variation influences rumen microbial methane production with best selection criterion for low methane emitting and efficiently feed converting hosts based on metagenomic gene abundance.","volume":"12","author":"Roehe","year":"2016","journal-title":"PLoS Genet."},{"key":"B54","doi-asserted-by":"publisher","first-page":"4682","DOI":"10.2527\/jas.2014-7573","article-title":"The potential of 3-nitrooxypropanol to lower enteric methane emissions from beef cattle1.","volume":"92","author":"Romero-Perez","year":"2014","journal-title":"J. Anim. Sci."},{"key":"B55","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1017\/S0007114514000932","article-title":"Hydrogen and methane emissions from beef cattle and their rumen microbial community vary with diet, time after feeding and genotype.","volume":"112","author":"Rooke","year":"2014","journal-title":"Br. J. Nutr."},{"key":"B56","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1128\/aem.48.1.211-217.1984","article-title":"Effect of carbon monoxide on fermentation of fiber, starch, and amino acids by mixed rumen microorganisms in vitro.","volume":"48","author":"Russell","year":"1984","journal-title":"Appl. Environ. Microbiol."},{"key":"B57","doi-asserted-by":"crossref","first-page":"1329","DOI":"10.2527\/jas1984.5951329x","article-title":"Effects of various methane inhibitors on the fementation of amino acids by mixed rumen microorganisms in vitro.","volume":"59","author":"Russell","year":"1984","journal-title":"J. Anim. Sci."},{"key":"B58","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1007\/978-94-009-1453-7_2","article-title":"The rumen bacteria","author":"Stewart","year":"1997","journal-title":"The Rumen Microbial Ecosystem"},{"key":"B59","doi-asserted-by":"crossref","first-page":"3378","DOI":"10.1093\/jn\/134.12.3378","article-title":"Development of an assay to quantify rumen ciliate protozoal biomass in cows using real-time PCR.","volume":"134","author":"Sylvester","year":"2004","journal-title":"J. Nutr."},{"key":"B60","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S0921-4488(98)00163-1","article-title":"An open-circuit hood system for gaseous exchange measurements in small ruminants.","volume":"32","author":"Takahashi","year":"1999","journal-title":"Small Rumin. Res."},{"key":"B61","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1128\/aem.54.1.20-29.1988","article-title":"Control of interspecies electron flow during anaerobic digestion: significance of formate transfer versus hydrogen transfer during syntrophic methanogenesis in flocs.","volume":"54","author":"Thiele","year":"1988","journal-title":"Appl. Environ. Microbiol."},{"key":"B62","doi-asserted-by":"crossref","first-page":"55","DOI":"10.3920\/9789086865666_003","article-title":"The role of thermodynamics in the control of ruminal fermentation","author":"Ungerfeld","year":"2006","journal-title":"Ruminant Physiology: Digestion, Metabolism and Impact of Nutrition on Gene Expression, Immunology and Stress"},{"key":"B63","doi-asserted-by":"publisher","first-page":"520","DOI":"10.1111\/j.1365-2672.2004.02330.x","article-title":"Effects of several inhibitors on pure cultures of ruminal methanogens.","volume":"97","author":"Ungerfeld","year":"2004","journal-title":"J. Appl. Microbiol."},{"issue":"319","key":"B64","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2013.00319","article-title":"A theoretical comparison between two ruminal electron sinks.","volume":"4","author":"Ungerfeld","year":"2013","journal-title":"Front. Microbiol."},{"issue":"37","key":"B65","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00037","article-title":"Shifts in metabolic hydrogen sinks in the methanogenesis-inhibited ruminal fermentation: a meta-analysis.","volume":"6","author":"Ungerfeld","year":"2015","journal-title":"Front. Microbiol."},{"issue":"e0140282","key":"B66","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140282","article-title":"Does dietary mitigation of enteric methane production affect rumen function and animal productivity in dairy cows?","volume":"10","author":"Veneman","year":"2015","journal-title":"PloS ONE"},{"key":"B67","doi-asserted-by":"publisher","DOI":"10.1071\/AN15705","article-title":"Optimal dose of 3-nitrooxypropanol for decreasing enteric methane emissions from beef cattle fed high-forage and high-grain diets.","author":"Vyas","year":"2016","journal-title":"Anim. Prod. Sci."},{"issue":"839","key":"B68","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-2032-0","article-title":"The rumen microbial metagenome associated with high methane production in cattle.","volume":"16","author":"Wallace","year":"2015","journal-title":"BMC Genomics"},{"key":"B69","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1007\/978-1-4020-6133-2_7","article-title":"A protocol for the operation of open-circuit chambers for measuring methane output in sheep","author":"Williams","year":"2007","journal-title":"Measuring Methane Production from Ruminants"},{"key":"B70","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1007\/978-94-009-1453-7_11","article-title":"Microbe-microbe interactions","author":"Wolin","year":"1997","journal-title":"The Rumen Microbial Ecosystem"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"deposited":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T08:47:09Z","timestamp":1718700429000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/Article\/10.3389\/fmicb.2016.01122\/abstract"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,7,19]]},"references-count":70,"URL":"https:\/\/doi.org\/10.3389\/fmicb.2016.01122","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,7,19]]}}}