{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:22:48Z","timestamp":1775064168909,"version":"3.50.1"},"reference-count":32,"publisher":"Frontiers Media SA","funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81371855"],"award-info":[{"award-number":["81371855"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2016.01304","type":"journal-article","created":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T23:04:25Z","timestamp":1471561465000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":17,"title":["Tigecycline-Amikacin Combination Effectively Suppresses the Selection of Resistance in Clinical Isolates of KPC-Producing Klebsiella pneumoniae"],"prefix":"10.3389","volume":"7","author":[{"given":"Wentao","family":"Ni","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanqi","family":"Wei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chufei","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beibei","family":"Liang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junchang","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Youning","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2016,8,19]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/jac\/22.Supplement_A.1","article-title":"General mechanisms of resistance to antibiotics.","volume":"22(Suppl. A)","author":"Bryan","year":"1988","journal-title":"J. Antimicrob. Chemother."},{"key":"B2","doi-asserted-by":"publisher","first-page":"477","DOI":"10.1016\/j.ijantimicag.2012.06.018","article-title":"Mutant prevention concentration of colistin alone and in combination with levofloxacin or tobramycin against multidrug-resistant Acinetobacter baumannii.","volume":"40","author":"Cai","year":"2012","journal-title":"Int. J. Antimicrob. Agents"},{"key":"B3","doi-asserted-by":"publisher","first-page":"5696","DOI":"10.1128\/aac.03110-14","article-title":"MgrB inactivation is a common mechanism of colistin resistance in KPC-producing Klebsiella pneumoniae of clinical origin.","volume":"58","author":"Cannatelli","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B4","doi-asserted-by":"publisher","first-page":"E23","DOI":"10.1111\/1469-0691.12070","article-title":"High rate of colistin resistance among patients with carbapenem-resistant Klebsiella pneumoniae infection accounts for an excess of mortality.","volume":"19","author":"Capone","year":"2013","journal-title":"Clin. Microbiol. Infect."},{"key":"B5","doi-asserted-by":"publisher","first-page":"4994","DOI":"10.1128\/aac.01009-12","article-title":"Breakthrough bacteremia due to extended-spectrum-beta-lactamase-producing Klebsiella pneumoniae during combination therapy with colistin and tigecycline.","volume":"56","author":"Cho","year":"2012","journal-title":"Antimicrob. Agents Chemother."},{"key":"B6","doi-asserted-by":"publisher","first-page":"275","DOI":"10.1093\/jac\/dkt315","article-title":"Mutant prevention concentrations of colistin for Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae clinical isolates.","volume":"69","author":"Choi","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"B7","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1093\/jac\/dku406","article-title":"Mutant prevention concentration of tigecycline for Acinetobacter baumannii and Klebsiella pneumoniae clinical isolates.","volume":"70","author":"Choi","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"B8","author":"CLSI","year":"2014","journal-title":"Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement (M100-S24)."},{"key":"B9","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1086\/600042","article-title":"Infection with panresistant Klebsiella pneumoniae: a report of 2 cases and a brief review of the literature.","volume":"49","author":"Elemam","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"B10","author":"EUCAST","year":"2015","journal-title":"The European Committee on Antimicrobial Susceptibility Testing. Breakpoint Tables for Interpretation of MICs and Zone Diameters, Version 5.0 EUCAST."},{"key":"B11","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1128\/aac.01222-13","article-title":"Antibiotic treatment of infections due to carbapenem-resistant Enterobacteriaceae: systematic evaluation of the available evidence.","volume":"58","author":"Falagas","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B12","doi-asserted-by":"publisher","first-page":"899","DOI":"10.1016\/j.cmi.2015.05.022","article-title":"Contemporary resistance trends and mechanisms for the old antibiotics colistin, temocillin, fosfomycin, mecillinam and nitrofurantoin.","volume":"21","author":"Giske","year":"2015","journal-title":"Clin. Microbiol. Infect."},{"issue":"e0119064","key":"B13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0119064","article-title":"Tigecycline susceptibility and the role of efflux pumps in tigecycline resistance in KPC-producing Klebsiella pneumoniae.","volume":"10","author":"He","year":"2015","journal-title":"PLoS ONE"},{"key":"B14","doi-asserted-by":"publisher","first-page":"1119","DOI":"10.1093\/jac\/dkq108","article-title":"Detection and treatment options for Klebsiella pneumoniae carbapenemases (KPCs): an emerging cause of multidrug-resistant infection.","volume":"65","author":"Hirsch","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"B15","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1016\/j.mib.2012.07.005","article-title":"Selection of resistance at lethal and non-lethal antibiotic concentrations.","volume":"15","author":"Hughes","year":"2012","journal-title":"Curr. Opin. Microbiol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1016\/j.ijantimicag.2013.08.008","article-title":"Rapid emergence of secondary resistance to gentamicin and colistin following selective digestive decontamination in patients with KPC-2-producing Klebsiella pneumoniae: a single-centre experience.","volume":"42","author":"L\u00fcbbert","year":"2013","journal-title":"Int. J. Antimicrob. Agents"},{"issue":"20939","key":"B17","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES2014.19.42.20939","article-title":"Colistin resistance superimposed to endemic carbapenem-resistant Klebsiella pneumoniae: a rapidly evolving problem in Italy, November 2013 to April 2014.","volume":"19","author":"Monaco","year":"2014","journal-title":"Euro. Surveill"},{"key":"B18","doi-asserted-by":"publisher","first-page":"785","DOI":"10.1016\/s1473-3099(13)70190-7","article-title":"Clinical epidemiology of the global expansion of Klebsiella pneumoniae carbapenemases.","volume":"13","author":"Munoz-Price","year":"2013","journal-title":"Lancet Infect. Dis."},{"key":"B19","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1016\/j.bjid.2014.12.004","article-title":"Efficacy of polymyxins in the treatment of carbapenem-resistant Enterobacteriaceae infections: a systematic review and meta-analysis.","volume":"19","author":"Ni","year":"2015","journal-title":"Braz. J. Infect. Dis."},{"key":"B20","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1038\/ja.2013.84","article-title":"In vitro effects of tigecycline in combination with colistin (polymyxin E) and sulbactam against multidrug-resistant Acinetobacter baumannii.","volume":"66","author":"Ni","year":"2013","journal-title":"J. Antibiot. (Tokyo)"},{"key":"B21","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1093\/jac\/dku323","article-title":"The mgrB gene as a key target for acquired resistance to colistin in Klebsiella pneumoniae.","volume":"70","author":"Poirel","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"B22","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1097\/QCO.0000000000000109","article-title":"Options for treating carbapenem-resistant Enterobacteriaceae.","volume":"27","author":"Rafailidis","year":"2014","journal-title":"Curr. Opin. Infect. Dis."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1754","DOI":"10.1128\/JB.182.6.1754-1756.2000","article-title":"AcrD of Escherichia coli is an aminoglycoside efflux pump.","volume":"182","author":"Rosenberg","year":"2000","journal-title":"J. Bacteriol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1093\/jac\/dkg255","article-title":"Stretching the mutant prevention concentration (MPC) beyond its limits.","volume":"51","author":"Smith","year":"2003","journal-title":"J. Antimicrob. Chemother."},{"key":"B25","doi-asserted-by":"publisher","first-page":"1404","DOI":"10.1128\/aem.01193-06","article-title":"Impact of antimicrobial usage on antimicrobial resistance in commensal Escherichia coli strains colonizing broiler chickens.","volume":"73","author":"Smith","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1016\/j.ijantimicag.2014.12.022","article-title":"Genetic characterisation of clinical Klebsiella pneumoniae isolates with reduced susceptibility to tigecycline: role of the global regulator RamA and its local repressor RamR.","volume":"45","author":"Wang","year":"2015","journal-title":"Int. J. Antimicrob. Agents"},{"key":"B27","author":"WHO","year":"2004","journal-title":"Laboratory Biosafety Manual-Third Edition. CLSI, Geneva."},{"key":"B28","doi-asserted-by":"publisher","first-page":"562","DOI":"10.1038\/ja.2015.30","article-title":"Investigation of the molecular epidemiology of Acinetobacter baumannii isolated from patients and environmental contamination.","volume":"68","author":"Ying","year":"2015","journal-title":"J. Antibiot. (Tokyo)"},{"key":"B29","doi-asserted-by":"publisher","first-page":"2228","DOI":"10.1128\/aac.01620-05","article-title":"Mutant prevention concentrations of levofloxacin alone and in combination with azithromycin, ceftazidime, colistin (Polymyxin E), meropenem, piperacillin-tazobactam, and tobramycin against Pseudomonas aeruginosa.","volume":"50","author":"Zhanel","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"B30","doi-asserted-by":"publisher","first-page":"S147","DOI":"10.1086\/321841","article-title":"Restricting the selection of antibiotic-resistant mutants: a general strategy derived from fluoroquinolone studies.","volume":"33(Suppl. 3)","author":"Zhao","year":"2001","journal-title":"Clin. Infect. Dis."},{"key":"B31","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1086\/338571","article-title":"Restricting the selection of antibiotic-resistant mutant bacteria: measurement and potential use of the mutant selection window.","volume":"185","author":"Zhao","year":"2002","journal-title":"J. Infect. Dis."},{"key":"B32","doi-asserted-by":"publisher","first-page":"434","DOI":"10.1093\/jac\/dkn229","article-title":"A unified anti-mutant dosing strategy.","volume":"62","author":"Zhao","year":"2008","journal-title":"J. Antimicrob. Chemother."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"deposited":{"date-parts":[[2017,6,24]],"date-time":"2017-06-24T16:59:14Z","timestamp":1498323554000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/Article\/10.3389\/fmicb.2016.01304\/abstract"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,19]]},"references-count":32,"URL":"https:\/\/doi.org\/10.3389\/fmicb.2016.01304","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,19]]}}}