{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T19:43:18Z","timestamp":1773171798682,"version":"3.50.1"},"reference-count":69,"publisher":"Frontiers Media SA","content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2017.01663","type":"journal-article","created":{"date-parts":[[2017,8,31]],"date-time":"2017-08-31T04:00:56Z","timestamp":1504152056000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":20,"title":["Infectious Agents As Markers of Human Migration toward the Amazon Region of Brazil"],"prefix":"10.3389","volume":"8","author":[{"given":"Ricardo","family":"Ishak","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz F. A.","family":"Machado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Izaura","family":"Cayres-Vallinoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marlu\u00edsa de O.","family":"Guimar\u00e3es Ishak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio C. R.","family":"Vallinoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2017,8,31]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"14542","DOI":"10.1073\/pnas.94.26.14542","article-title":"Asian genotypes of JC virus in Native Americans and in a Pacific Island population: markers of viral evolution and human migration.","volume":"94","author":"Agostini","year":"1997","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B2","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1089\/AID.2013.0181","article-title":"Molecular characterization of the human T cell lymphotropic virus type 2 long terminal repeat region: a discussion about possible influences at viral gene expression.","volume":"30","author":"Barreto","year":"2014","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"B3","doi-asserted-by":"publisher","first-page":"1562","DOI":"10.1086\/315456","article-title":"Human herpesvirus 8 in Brazilian Amerindians: a hyperendemic population with a new subtype.","volume":"181","author":"Biggar","year":"2000","journal-title":"J. Infect. Dis."},{"key":"B4","first-page":"631","article-title":"Endemic HTLV-II infection among Tobas and Mataco Amerindians from North Argentina.","volume":"6","author":"Biglione","year":"1993","journal-title":"J. Acquir. Immune Defic. Syndr."},{"key":"B5","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1128\/IAI.58.1.93-97.1990","article-title":"Structural and antigenic analysis of Chlamydia pneumoniae.","volume":"58","author":"Campbell","year":"1990","journal-title":"Infect. Immun."},{"key":"B6","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1089\/aid.2005.21.521","article-title":"HTLV-2 horizontal and vertical transmission in a family from a Brazilian urban area: seroepidemiological, clinical and molecular study.","volume":"21","author":"Catalan-Soares","year":"2005","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0046523","article-title":"Human JCV infections as a bio-anthropological marker of the formation of Brazilian Amazonian populations.","volume":"7","author":"Cayres-Vallinoto","year":"2012","journal-title":"PLoS ONE"},{"key":"B8","doi-asserted-by":"publisher","first-page":"360","DOI":"10.1016\/j.bjid.2016.05.005","article-title":"JC virus\/human immunodeficiency virus 1 co-infection in the Brazilian Amazonian region.","volume":"20","author":"Cayres-Vallinoto","year":"2016","journal-title":"Braz. J. Infect. Dis."},{"key":"B9","doi-asserted-by":"publisher","first-page":"1865","DOI":"10.1126\/science.7997879","article-title":"Identification of herpesvirus-like DNA sequences in AIDS-associated Kaposi\u2019s sarcoma.","volume":"266","author":"Chang","year":"1994","journal-title":"Science"},{"key":"B10","doi-asserted-by":"publisher","first-page":"615","DOI":"10.1590\/S0074-02761998000500010","article-title":"Molecular epidemiology of human polyomavirus JC in the Biaka Pygmies and Bantu of Central Africa.","volume":"93","author":"Chima","year":"1998","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"B11","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/S0140-6736(84)92113-5","article-title":"Chlamydial serology in fertile and infertile women.","volume":"1","author":"Conway","year":"1984","journal-title":"Lancet"},{"key":"B12","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.1097\/00002030-199907090-00004","article-title":"Variability and evolution of Kaposi\u2019s sarcoma-associated herpesvirus in Europe and Africa.","volume":"13","author":"Cook","year":"1999","journal-title":"AIDS"},{"key":"B13","doi-asserted-by":"publisher","first-page":"2433","DOI":"10.1099\/vir.0.81087-0","article-title":"Increasing seroprevalence of human herpesvirus 8 (HHV-8) with age confirms HHV-8 endemicity in Amazon Amerindians from Brazil.","volume":"86","author":"Cunha","year":"2005","journal-title":"J. Gen. Virol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"641","DOI":"10.1016\/S1473-3099(11)70093-7","article-title":"Palaeogenomics of Mycobacterium tuberculosis: epidemic bursts with a degrading genome.","volume":"11","author":"Djelouadji","year":"2011","journal-title":"Lancet Infect. Dis."},{"key":"B15","doi-asserted-by":"publisher","first-page":"12637","DOI":"10.1073\/pnas.95.21.12637","article-title":"Detection of 400-year-old Yersinia pestis DNA in human dental pulp: an approach to the diagnosis of ancient septicemia.","volume":"95","author":"Drancourt","year":"1998","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B16","doi-asserted-by":"crossref","first-page":"1481","DOI":"10.1128\/JVI.70.3.1481-1492.1996","article-title":"Identification and characterization of a new and distinct molecular subtype of human T-cell lymphotropic virus type 2.","volume":"70","author":"Eiraku","year":"1996","journal-title":"J. Virol."},{"key":"B17","author":"Emmi","year":"2013","journal-title":"Um S\u00e9culo de Imigra\u00e7\u00f5es Internacionais na Amaz\u00f4nia Brasileira (1850-1950)."},{"key":"B18","doi-asserted-by":"publisher","first-page":"576","DOI":"10.1006\/viro.1993.1631","article-title":"High rate infection with the human T-leukemia retrovirus type II in four Indian populations of Argentina.","volume":"197","author":"Ferrer","year":"1993","journal-title":"Virology"},{"key":"B19","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)14487-X","article-title":"Human papillomavirus in a 16th century mummy.","volume":"362","author":"Fornaciari","year":"2003","journal-title":"Lancet"},{"key":"B20","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1590\/S0036-46652002000600003","article-title":"Prevalence of human herpesvirus 8 antibodies in the population of Belem, Para, Brazil.","volume":"44","author":"Freitas","year":"2002","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"B21","doi-asserted-by":"publisher","first-page":"733","DOI":"10.1016\/0030-4220(91)90284-J","article-title":"Human immunodeficiency virus infection of fibroblasts of dental pulp in seropositive patients.","volume":"71","author":"Glick","year":"1991","journal-title":"Oral Surg. Oral Med. Oral Pathol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1093\/infdis\/132.1.87","article-title":"New knowledge of chlamydiae and the diseases they cause.","volume":"132","author":"Grayston","year":"1975","journal-title":"J. Infect. Dis."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199102213240815","article-title":"HTLV-II endemicity among Guaymi Indians in Panama.","volume":"324","author":"Heneine","year":"1991","journal-title":"N. Engl. J. Med."},{"key":"B24","doi-asserted-by":"publisher","first-page":"170","DOI":"10.1590\/0037-8682-0038-2015","volume":"48","author":"Ishak","year":"2015","journal-title":"Chlamydia trachomatis"},{"key":"B25","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1590\/S0102-311X2001000200013","article-title":"Chlamydia infection impact among native Indian groups of the Brazilian Amazon region.","volume":"17","author":"Ishak","year":"2001","journal-title":"Cad. Saude Publica"},{"key":"B26","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/0035-9203(93)90421-L","article-title":"Chlamydial infection in the Amazon region of Brazil.","volume":"87","author":"Ishak","year":"1993","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"B27","doi-asserted-by":"publisher","first-page":"1537","DOI":"10.1002\/jmv.20995","article-title":"High diversity of HHV-8 molecular subtypes in the Amazon region of Brazil: evidence of an ancient human infection.","volume":"79","author":"Ishak","year":"2007","journal-title":"J. Med. Virol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"40","DOI":"10.1590\/S0036-46651988000100007","article-title":"Prevalence of antibodies to Chlamydia trachomatis in population groups of Brazil, England and Portugal.","volume":"30","author":"Ishak","year":"1988","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"B29","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1089\/aid.1995.11.813","article-title":"Identification of human T cell lymphotropic virus type IIa infection in the Kayapo, an indigenous population of Brazil.","volume":"11","author":"Ishak","year":"1995","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"B30","doi-asserted-by":"publisher","first-page":"80","DOI":"10.1590\/S0036-46651989000200003","article-title":"Soroepidemiologia retrospectiva do HIV-1.","volume":"31","author":"Ishak","year":"1989","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"B31","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1590\/S0037-86822001000600004","article-title":"Molecular evidence of mother-to-child transmission of HTLV-IIc in the Kararao Village (Kayapo) in the Amazon region of Brazil.","volume":"34","author":"Ishak","year":"2001","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"B32","doi-asserted-by":"publisher","first-page":"3478","DOI":"10.1128\/JVI.74.8.3478-3485.2000","article-title":"Identification of antigenic proteins encoded by human herpesvirus 8 and seroprevalence in the general population and among patients with and without Kaposi\u2019s sarcoma.","volume":"74","author":"Katano","year":"2000","journal-title":"J. Virol."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1525","DOI":"10.1086\/491744","article-title":"Serological and molecular evidence that human herpesvirus 8 is endemic among Amerindians in French Guiana.","volume":"192","author":"Kazanji","year":"2005","journal-title":"J. Infect. Dis."},{"key":"B34","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1128\/JCM.33.6.1448-1451.1995","article-title":"Parent-to-children transmission is relatively common in the spread of the human polyomavirus JC virus.","volume":"33","author":"Kunitake","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"B35","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1089\/aid.1993.9.123","article-title":"HTLV-II infection in Florida Indians.","volume":"9","author":"Levine","year":"1993","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"B36","doi-asserted-by":"publisher","first-page":"1428","DOI":"10.1038\/71006","article-title":"The presence of ancient human T-cell lymphotropic virus type I provirus DNA in an Andean mummy.","volume":"5","author":"Li","year":"1999","journal-title":"Nat. Med."},{"key":"B37","first-page":"145","article-title":"Seroprevalencia de infecci\u00f3n por Chlamydia pneumoniae en poblaci\u00f3n sana de Santiago de Chile.","volume":"12","author":"Lobos","year":"1996","journal-title":"Rev. Chil. Enfermid. Resp."},{"key":"B38","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1089\/aid.2006.22.1267","article-title":"Serological and molecular typing of HIV type 1 infection in the Tiriyo tribe, a native Indian community of the Amazon region of Brazil.","volume":"22","author":"Machado","year":"2006","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"B39","doi-asserted-by":"publisher","first-page":"100","DOI":"10.1093\/infdis\/166.1.100","article-title":"Endemic human T cell lymphotropic virus type II infection among isolated Brazilian Amerindians.","volume":"166","author":"Maloney","year":"1992","journal-title":"J. Infect. Dis."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1947","DOI":"10.1590\/S0102-311X2005000600045","article-title":"Human T-cell lymphotropic virus type II in Guarani Indians, Southern Brazil.","volume":"21","author":"Menna-Barreto","year":"2005","journal-title":"Cad. Saude Publica"},{"key":"B41","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1590\/S0036-46652012000400004","article-title":"High prevalence of the simultaneous excretion of polyomaviruses JC and BK in the urine of HIV-infected patients without neurological symptoms in S\u00e3o Paulo, Brazil.","volume":"54","author":"Nali","year":"2012","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"B42","doi-asserted-by":"publisher","first-page":"2756","DOI":"10.1590\/S0102-311X2007001100023","article-title":"Preval\u00eancia dos marcadores sorol\u00f3gicos dos v\u00edrus das hepatites B e D na \u00e1rea ind\u00edgena Apyterewa, do grupo Parakan\u00e3, Par\u00e1, Brasil.","volume":"23","author":"Nunes","year":"2007","journal-title":"Cad. Sa\u00fade P\u00fablica"},{"key":"B43","doi-asserted-by":"publisher","first-page":"12800","DOI":"10.1073\/pnas.220225197","article-title":"Molecular identification by \u201csuicide PCR\u201d of Yersinia pestis as the agent of medieval black death.","volume":"97","author":"Raoult","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B44","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-540-75855-6","author":"Raoult","year":"2008","journal-title":"Paleomicrobiology - Past Human Infections."},{"key":"B45","doi-asserted-by":"publisher","first-page":"1651","DOI":"10.1073\/pnas.96.4.1651","article-title":"Origin and evolution of the 1918 Spanish influenza virus hemagglutinin gene.","volume":"96","author":"Reid","year":"1999","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B46","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1089\/aid.2006.22.301","article-title":"Molecular evidence of HTLV-II subtype B among an urban population living in South Brazil.","volume":"22","author":"Renner","year":"2006","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"B47","author":"Rodrigues","year":"1999","journal-title":"An\u00e1lise"},{"key":"B48","author":"Salles","year":"1998","journal-title":"O Negro no Par\u00e1 sob o Regime de Escravid\u00e3o."},{"key":"B49","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1590\/S0074-02761995000400001","article-title":"A possible correlation between the host genetic background in the epidemiology of hepatitis B virus in the Amazon region of Brazil.","volume":"90","author":"Santos","year":"1995","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"B50","first-page":"311","article-title":"The indigenous contribution to the formation of the population of the Brazilian Amazon Region.","volume":"18","author":"Santos","year":"1995","journal-title":"Braz. J. Genet."},{"key":"B51","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1002\/jmv.2138","article-title":"HTLV-II infection associated with a chronic neurodegenerative disease: clinical and molecular analysis.","volume":"66","author":"Silva","year":"2002","journal-title":"J. Med. Virol."},{"key":"B52","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165272.","article-title":"Detection of HCV persistent infections in the dental pulp: a novel approach for the detection of past and ancient infections.","volume":"11","author":"Siravenha","year":"2016","journal-title":"PLoS ONE"},{"key":"B53","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1002\/oa.1390030211","article-title":"The use of the polymerase chain reaction (PCR) to detect Mycobacterium tuberculosis in ancient skeletons.","volume":"3","author":"Spigelman","year":"1993","journal-title":"Int. J. Osteoarchaeol."},{"key":"B54","doi-asserted-by":"publisher","first-page":"1905","DOI":"10.1016\/S1286-4579(00)01339-3","article-title":"JC virus as a marker of human migration to the Americas.","volume":"2","author":"Stoner","year":"2000","journal-title":"Microbes Infect."},{"key":"B55","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1007\/s00239-001-2329-2","article-title":"JC virus strains Indigenous to Northeastern Siberians and Canadian Inuits are unique but evolutionally related to those distributed throughout Europe and Mediterranean areas.","volume":"55","author":"Sugimoto","year":"2002","journal-title":"J. Mol. Evol."},{"key":"B56","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1089\/aid.1996.12.635","article-title":"Endemicity and phylogeny of the human T cell lymphotropic virus type II subtype A from the Kayapo Indians of Brazil: evidence for limited regional dissemination.","volume":"12","author":"Switzer","year":"1996","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"B57","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1007\/BF01702596","article-title":"Molecular analysis of human T-cell lymphotropic virus subtype II from Wayu Indians of Colombia demonstrates two subtypes of HTLV-IIb.","volume":"10","author":"Switzer","year":"1995","journal-title":"Virus Gene"},{"key":"B58","doi-asserted-by":"publisher","first-page":"465","DOI":"10.1002\/ajpa.20208","article-title":"Phylogenetic analysis of major African genotype (Af2) of JC virus: implications for origin and dispersals of modern Africans.","volume":"129","author":"Takasaka","year":"2006","journal-title":"Am. J. Phys. Anthropol."},{"key":"B59","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1093\/oxfordjournals.bmb.a071815","article-title":"Chlamydial serology.","volume":"39","author":"Treharne","year":"1983","journal-title":"Br. Med. Bull."},{"key":"B60","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1353\/hub.2002.0059","article-title":"Molecular epidemiology of human T-lymphotropic virus type II infection in Amerindian and urban populations of the Amazon region of Brazil.","volume":"74","author":"Vallinoto","year":"2002","journal-title":"Hum. Biol."},{"key":"B61","doi-asserted-by":"crossref","first-page":"156","DOI":"10.7883\/yoken.JJID.2004.156","article-title":"Serological and molecular evidence of HTLV-I infection among japanese immigrants living in the Amazon region of Brazil.","volume":"57","author":"Vallinoto","year":"2004","journal-title":"Jpn. J. Infect. Dis."},{"key":"B62","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1093\/infdis\/130.4.388","article-title":"Human serology in Chlamydia trachomatis infection with microimmunofluorescence.","volume":"130","author":"Wang","year":"1974","journal-title":"J. Infect. Dis."},{"key":"B63","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1002\/jmv.20005","article-title":"Simultaneous detection and differentiation of human polyomaviruses JC and BK by a rapid and sensitive PCR-ELAHA assay and a survey of the JCV subtypes within an Australian population.","volume":"72","author":"Whiley","year":"2004","journal-title":"J. Med. Virol."},{"key":"B64","doi-asserted-by":"publisher","first-page":"4357","DOI":"10.1128\/JCM.39.12.4357-4361.2001","article-title":"Detection and differentiation of human polyomaviruses JC and BK by LightCycler PCR.","volume":"39","author":"Whiley","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"B65","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1099\/vir.0.19465-0","article-title":"Genotypic characterization of Kaposi\u2019s sarcoma-associated herpesvirus in asymptomatic infected subjects from isolated populations.","volume":"85","author":"Whitby","year":"2004","journal-title":"J. Gen. Virol."},{"key":"B66","first-page":"59","article-title":"Molecular epidemiology of human T-cell leukemia virus type I (HTLV-1) Brazil: the predominant HTLV-1s in South America differ from HTLV-ls of Japan and Africa, as well as those of Japanese immigrants and their relatives in Brazil.","volume":"261","author":"Yamashita","year":"1999","journal-title":"Virology"},{"key":"B67","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1089\/aid.1994.10.97","article-title":"Geographic independence of HTLV-I and HTLV- II foci in the Andes Highland, the Atlantic Coast, and the Orinoco of Columbia.","volume":"10","author":"Zaninovic","year":"1994","journal-title":"AIDS Res. Hum. Retroviruses"},{"key":"B68","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1007\/s00239-002-2376-3","article-title":"Phylogenetic relationships among JC virus strains in Japanese\/Koreans and Native Americans speaking Amerind or Na-Dene.","volume":"56","author":"Zheng","year":"2003","journal-title":"J. Mol. Evol."},{"key":"B69","doi-asserted-by":"crossref","first-page":"4156","DOI":"10.1128\/JVI.73.5.4156-4170.1999","article-title":"High-level variability in the ORF-K1 membrane protein gene at the left end of the Kaposi\u2019s sarcoma-associated herpesvirus genome defines four major virus subtypes and multiple variants or clades in different human populations.","volume":"73","author":"Zong","year":"1999","journal-title":"J. Virol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fmicb.2017.01663\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,25]],"date-time":"2025-06-25T09:38:44Z","timestamp":1750844324000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fmicb.2017.01663\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,31]]},"references-count":69,"alternative-id":["10.3389\/fmicb.2017.01663"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2017.01663","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,8,31]]},"article-number":"1663"}}