{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T20:48:42Z","timestamp":1790628522220,"version":"4.1.0"},"reference-count":49,"publisher":"Frontiers Media SA","funder":[{"DOI":"10.13039\/501100001054","name":"Meat and Livestock Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003526","name":"Department of Agriculture, Australian Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003526","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2017.01871","type":"journal-article","created":{"date-parts":[[2017,10,5]],"date-time":"2017-10-05T06:51:19Z","timestamp":1507186279000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":58,"title":["Phloroglucinol Degradation in the Rumen Promotes the Capture of Excess Hydrogen Generated from Methanogenesis Inhibition"],"prefix":"10.3389","volume":"8","author":[{"given":"Gonzalo","family":"Martinez-Fernandez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart E.","family":"Denman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane","family":"Cheung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher S.","family":"McSweeney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2017,10,5]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1016\/S0960-8524(03)00086-5","article-title":"Effect of select nitrocompounds on ruminal fermentation: an initial look at their potential to reduce economic and environmental costs associated with ruminal methanogenesis.","volume":"90","author":"Anderson","year":"2003","journal-title":"Bioresour. Technol."},{"key":"B2","year":"2005","journal-title":"Official Methods of Analysis"},{"key":"B3","doi-asserted-by":"publisher","first-page":"576","DOI":"10.2508\/chikusan.69.576","article-title":"Formate metabolism by ruminal microorganisms in relation to methanogenesis.","volume":"69","author":"Asanuma","year":"1998","journal-title":"Anim. Sci. Technol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1007\/BF00173998","article-title":"Formate production and utilization by methanogens and by sewage sludge consortia-interference with the concept of interspecies formate transfer.","volume":"40","author":"Bleicher","year":"1994","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"B5","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/1-4020-3791-0_11","article-title":"\u201cAnaerobic fungal populations,\u201d in","author":"Brookman","year":"2005","journal-title":"Methods in Gut Microbial Ecology for Ruminants"},{"key":"B6","doi-asserted-by":"publisher","first-page":"585","DOI":"10.2527\/jas1977.453585x","article-title":"Manipulating rumen fermentation.","volume":"45","author":"Chalupa","year":"1977","journal-title":"J. Anim. Sci."},{"key":"B7","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1093\/clinchem\/8.2.130","article-title":"Modified reagents for determination of urea and ammonia.","volume":"8","author":"Chaney","year":"1962","journal-title":"Clin. Chem."},{"key":"B8","doi-asserted-by":"publisher","first-page":"15760","DOI":"10.1002\/anie.201607494","article-title":"Biocatalytic properties and structural analysis of phloroglucinol reductases.","volume":"128","author":"Conradt","year":"2016","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"B9","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01087","article-title":"Metagenomic analysis of the rumen microbial community following inhibition of methane formation by a halogenated methane analogue.","volume":"6","author":"Denman","year":"2015","journal-title":"Front. Microbiol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"2460","DOI":"10.1093\/bioinformatics\/btq461","article-title":"Search and clustering orders of magnitude faster than BLAST.","volume":"26","author":"Edgar","year":"2010","journal-title":"Bioinformatics"},{"key":"B11","doi-asserted-by":"publisher","first-page":"6391","DOI":"10.1128\/AEM.00181-07","article-title":"Establishment and development of ruminal hydrogenotrophs in methanogen-free lambs.","volume":"73","author":"Fonty","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"B12","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1079\/9781780644325.0292","article-title":"\u201cAcetogenesis as an alternative to methanogenesis in the rumen,\u201d in","author":"Gagen","year":"2015","journal-title":"Livestock Production and Climate Change"},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-014-0314-3","article-title":"Investigation of a new acetogen isolated from an enrichment of the tammar wallaby forestomach.","volume":"14","author":"Gagen","year":"2014","journal-title":"BMC Microbiol."},{"key":"B14","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1017\/S1751731113000876","article-title":"Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: a review.","volume":"7","author":"Gerber","year":"2013","journal-title":"Animal"},{"key":"B15","doi-asserted-by":"publisher","first-page":"10663","DOI":"10.1073\/pnas.1504124112","article-title":"An inhibitor persistently decreased enteric methane emission from dairy cows with no negative effect on milk production.","volume":"112","author":"Hristov","year":"2015","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B16","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1128\/JB.102.2.389-397.1970","article-title":"Formate as an intermediate in the bovine rumen fermentation.","volume":"102","author":"Hungate","year":"1970","journal-title":"J. Bacteriol."},{"key":"B17","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.anifeedsci.2010.07.002","article-title":"Influence of hydrogen on rumen methane formation and fermentation balances through microbial growth kinetics and fermentation thermodynamics.","volume":"160","author":"Janssen","year":"2010","journal-title":"Anim. Feed Sci. Technol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1071\/AR99004","article-title":"Ruminal acetogens and their potential to lower ruminant methane emissions.","volume":"50","author":"Joblin","year":"1999","journal-title":"Aust. J. Agric. Res."},{"key":"B19","doi-asserted-by":"publisher","first-page":"2483","DOI":"10.2527\/1995.7382483x","article-title":"Methane emissions from cattle.","volume":"73","author":"Johnson","year":"1995","journal-title":"J. Anim. Sci."},{"key":"B20","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1007\/BF00414999","article-title":"Fermentative metabolism of substituted monoaromatic compounds by a bacterial community from anaerobic sediments.","volume":"133","author":"Kaiser","year":"1982","journal-title":"Arch. Microbiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform.","volume":"79","author":"Kozich","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1007\/BF00454948","article-title":"Eubacterium-Oxidoreducens Sp-Nov requiring H2 or formate to degrade gallate, pyrogallol, phloroglucinol and quercetin.","volume":"144","author":"Krumholz","year":"1986","journal-title":"Arch. Microbiol."},{"key":"B23","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00228","article-title":"Insights on alterations to the rumen ecosystem by nitrate and nitrocompounds.","volume":"7","author":"Latham","year":"2016","journal-title":"Front. Microbiol."},{"key":"B24","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1071\/AN13381","article-title":"Interactions between microbial consortia in biofilms: a paradigm shift in rumen microbial ecology and enteric methane mitigation.","volume":"54","author":"Leng","year":"2014","journal-title":"Anim. Prod. Sci."},{"key":"B25","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"B26","doi-asserted-by":"publisher","first-page":"646","DOI":"10.1139\/m81-099","article-title":"Identification of rumen bacteria that anaerobically degrade aliphatic nitrotoxins.","volume":"27","author":"Majak","year":"1981","journal-title":"Can. J. Microbiol."},{"key":"B27","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01122","article-title":"Methane inhibition alters the microbial community, hydrogen flow, and fermentation response in the rumen of cattle.","volume":"7","author":"Martinez-Fernandez","year":"2016","journal-title":"Front. Microbiol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1038\/ismej.2011.139","article-title":"An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea.","volume":"6","author":"McDonald","year":"2012","journal-title":"ISME J."},{"key":"B29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061217","article-title":"phyloseq: an R package for reproducible interactive analysis and graphics of microbiome census data.","volume":"8","author":"McMurdie","year":"2013","journal-title":"PLOS ONE"},{"key":"B30","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1016\/S0377-8401(01)00232-2","article-title":"Microbial interactions with tannins: nutritional consequences for ruminants.","volume":"91","author":"McSweeney","year":"2001","journal-title":"Anim. Feed Sci. Technol."},{"key":"B31","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1128\/AEM.27.5.985-987.1974","article-title":"A serum bottle modification of the Hungate technique for cultivating obligate anaerobes.","volume":"27","author":"Miller","year":"1974","journal-title":"Appl. Microbiol."},{"key":"B32","doi-asserted-by":"publisher","first-page":"482","DOI":"10.1017\/S0007114511005794","article-title":"Responses in digestion, rumen fermentation and microbial populations to inhibition of methane formation by a halogenated methane analogue.","volume":"108","author":"Mitsumori","year":"2012","journal-title":"Br. J. Nutr."},{"key":"B33","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1006\/anae.1996.0023","article-title":"In vitro interactions between rumen H2-producing cellulolytic microorganisms and H2-utilizing acetogenic and sulfate-reducing bacteria.","volume":"2","author":"Morvan","year":"1996","journal-title":"Anaerobe"},{"key":"B34","doi-asserted-by":"publisher","first-page":"1307","DOI":"10.1071\/AR9921307","article-title":"Metabolism in sheep of gallic acid, tannic-acid and hydrolyzable tannin from Terminalia oblongata.","volume":"43","author":"Murdiati","year":"1992","journal-title":"Aust. J. Agric. Res."},{"key":"B35","doi-asserted-by":"publisher","first-page":"27","DOI":"10.1079\/BJN20051445","article-title":"Propionate precursors and other metabolic intermediates as possible alternative electron acceptors to methanogenesis in ruminal fermentation in vitro.","volume":"94","author":"Newbold","year":"2005","journal-title":"Br. J. Nutr."},{"key":"B36","author":"Oksanen","year":"2013","journal-title":"Package \u2018vegan\u2019. Community Ecology Package, Version 2"},{"key":"B37","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/418912","article-title":"Polyphenol-rich fraction of brown alga Ecklonia cava collected from Gijang, Korea, reduces obesity and glucose levels in high-fat diet-induced obese mice.","volume":"2012","author":"Park","year":"2012","journal-title":"Evid. Based Complement. Alternat. Med."},{"key":"B38","doi-asserted-by":"crossref","first-page":"1010","DOI":"10.1128\/AEM.42.6.1010-1017.1981","article-title":"Catabolism of phloroglucinol by the rumen anaerobe Coprococcus.","volume":"42","author":"Patel","year":"1981","journal-title":"Appl. Environ. Microbiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"2958","DOI":"10.1038\/ismej.2016.62","article-title":"Specific microbiome-dependent mechanisms underlie the energy harvest efficiency of ruminants.","volume":"10","author":"Shabat","year":"2016","journal-title":"ISME J."},{"key":"B40","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1128\/AEM.63.2.734-742.1997","article-title":"Competition for cellulose among three predominant ruminal cellulolytic bacteria under substrate-excess and substrate-limited conditions.","volume":"63","author":"Shi","year":"1997","journal-title":"Appl. Environ. Microbiol."},{"key":"B41","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1128\/AEM.54.1.20-29.1988","article-title":"Control of interspecies electron flow during anaerobic digestion: significance of formate transfer versus hydrogen transfer during syntrophic methanogenesis in flocs.","volume":"54","author":"Thiele","year":"1988","journal-title":"Appl. Environ. Microbiol."},{"key":"B42","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1139\/m76-022","article-title":"Products of anaerobic phloroglucinol degradation by Coprococcus Sp Pe15.","volume":"22","author":"Tsai","year":"1976","journal-title":"Can. J. Microbiol."},{"key":"B43","doi-asserted-by":"publisher","first-page":"794","DOI":"10.1139\/m75-117","article-title":"Isolation and identification of rumen bacteria capable of anaerobic phloroglucinol degradation.","volume":"21","author":"Tsai","year":"1975","journal-title":"Can. J. Microbiol."},{"key":"B44","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.01272","article-title":"Limits to dihydrogen incorporation into electron sinks alternative to methanogenesis in ruminal fermentation.","volume":"6","author":"Ungerfeld","year":"","journal-title":"Front. Microbiol."},{"key":"B45","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2015.00037","article-title":"Shifts in metabolic hydrogen sinks in the methanogenesis-inhibited ruminal fermentation: a meta-analysis.","volume":"6","author":"Ungerfeld","year":"","journal-title":"Front. Microbiol."},{"key":"B46","doi-asserted-by":"publisher","first-page":"5856","DOI":"10.3168\/jds.2010-3281","article-title":"Nitrate and sulfate: effective alternative hydrogen sinks for mitigation of ruminal methane production in sheep.","volume":"93","author":"van Zijderveld","year":"2010","journal-title":"J. Dairy Sci."},{"key":"B47","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-2032-0","article-title":"The rumen microbial metagenome associated with high methane production in cattle.","volume":"16","author":"Wallace","year":"2015","journal-title":"BMC Genomics"},{"key":"B48","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-319-24277-4","author":"Wickham","year":"2016","journal-title":"ggplot2: Elegant Graphics for Data Analysis"},{"key":"B49","doi-asserted-by":"publisher","first-page":"467","DOI":"10.1007\/978-94-009-1453-7_11","article-title":"\u201cMicrobe-microbe interactions,\u201d in","author":"Wolin","year":"1997","journal-title":"The Rumen Microbial Ecosystem"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fmicb.2017.01871\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,10,18]],"date-time":"2020-10-18T19:22:04Z","timestamp":1603048924000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fmicb.2017.01871\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,5]]},"references-count":49,"alternative-id":["10.3389\/fmicb.2017.01871"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2017.01871","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,10,5]]},"article-number":"1871"}}