{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T14:41:22Z","timestamp":1790520082931,"version":"4.1.0"},"reference-count":50,"publisher":"Frontiers Media SA","funder":[{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["AGL2015-65010-C3-1-R"],"award-info":[{"award-number":["AGL2015-65010-C3-1-R"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministerio de Econom\u00eda y Competitividad","doi-asserted-by":"publisher","award":["BIO2015-69085-REDC"],"award-info":[{"award-number":["BIO2015-69085-REDC"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2017.02367","type":"journal-article","created":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T04:23:25Z","timestamp":1512102205000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":8,"title":["Successful Establishment of Plasmids R1 and pMV158 in a New Host Requires the Relief of the Transcriptional Repression of Their Essential rep Genes"],"prefix":"10.3389","volume":"8","author":[{"given":"Jos\u00e9 \u00c1.","family":"Ruiz-Mas\u00f3","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis M.","family":"Luengo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Inmaculada","family":"Moreno-C\u00f3rdoba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ram\u00f3n","family":"D\u00edaz-Orejas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gloria","family":"del Solar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2017,12,1]]},"reference":[{"key":"B1","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1093\/genetics\/39.4.440","article-title":"Segregation of new lysogenic types during growth of a doubly lysogenic strain derived from Escherichia coli K12","volume":"39","author":"Appleyard","year":"1954","journal-title":"Genetics"},{"key":"B2","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1006\/plas.1998.1386","article-title":"Role of the rom protein in copy number control of plasmid pBR322 at different growth rates in Escherichia coli K-12","volume":"41","author":"Atlung","year":"1999","journal-title":"Plasmid"},{"key":"B3","doi-asserted-by":"publisher","first-page":"e00662","DOI":"10.1128\/mBio.00662-15","article-title":"The ParB-parS chromosome segregation system modulates competence development in Streptococcus pneumoniae","volume":"6","author":"Attaiech","year":"2015","journal-title":"MBio"},{"key":"B4","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1111\/j.1574-6968.1990.tb03997.x","article-title":"Transformation of Staphylococcus epidermidis and other staphylococcal species with plasmid DNA by electroporation","volume":"54","author":"Augustin","year":"1990","journal-title":"FEMS Microbiol. Lett."},{"key":"B5","doi-asserted-by":"publisher","first-page":"20915","DOI":"10.1038\/srep20915","article-title":"Conformational plasticity of RepB, the replication initiator protein of promiscuous streptococcal plasmid pMV158","volume":"6","author":"Boer","year":"2016","journal-title":"Sci. Rep."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1666","DOI":"10.1038\/emboj.2009.125","article-title":"Plasmid replication initiator RepB forms a hexamer reminiscent of ring helicases and has mobile nuclease domains","volume":"28","author":"Boer","year":"2009","journal-title":"EMBO J."},{"key":"B7","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.PLAS-0001-2013","article-title":"Plasmid replication control by antisense RNAs","author":"Brantl","year":"2014","journal-title":"Microbiol. Spectr."},{"key":"B8","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1128\/AAC.18.5.753","article-title":"Identification of tetracycline-resistant R-plasmids in Streptococcus agalactiae (group B)","volume":"18","author":"Burdett","year":"1980","journal-title":"Antimicrob. Agents Chemother."},{"key":"B9","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.1128\/JB.134.3.1141-1156.1978","article-title":"Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid","volume":"134","author":"Chang","year":"1978","journal-title":"J. Bacteriol."},{"key":"B10","doi-asserted-by":"publisher","first-page":"2856","DOI":"10.1073\/pnas.0409790102","article-title":"Multiple homeostatic mechanisms in the control of P1 plasmid replication","volume":"102","author":"Das","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B11","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1111\/j.1365-2958.1995.18050913.x","article-title":"Replication control of plasmid pLS1: efficient regulation of plasmid copy number is exerted by the combined action of two plasmid components, CopG and RNA II","volume":"18","author":"del Solar","year":"1995","journal-title":"Mol. Microbiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1046\/j.1365-2958.2000.02005.x","article-title":"Plasmid copy number control: an ever-growing story","volume":"37","author":"del Solar","year":"2000","journal-title":"Mol. Microbiol."},{"key":"B13","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/BF00282785","article-title":"In vivo definition of the functional origin of replication (ori(+)) of the promiscuous plasmid pLS1","volume":"237","author":"del Solar","year":"1993","journal-title":"Mol. Gen. Genet."},{"key":"B14","doi-asserted-by":"crossref","first-page":"12569","DOI":"10.1016\/S0021-9258(19)38382-6","article-title":"Plasmid pLS1-encoded RepA protein regulates transcription from repAB promoter by binding to a DNA sequence containing a 13-base pair symmetric element","volume":"265","author":"del Solar","year":"1990","journal-title":"J. Biol. Chem."},{"key":"B15","doi-asserted-by":"publisher","first-page":"5561","DOI":"10.1093\/nar\/15.14.5561","article-title":"Initiation signals for the conversion of single stranded to double stranded DNA forms in the streptococcal plasmid pLS1","volume":"15","author":"del Solar","year":"1987","journal-title":"Nucleic Acids Res."},{"key":"B16","doi-asserted-by":"publisher","first-page":"6127","DOI":"10.1093\/nar\/16.13.6127","article-title":"High efficiency transformation of E. coli by high voltage electroporation","volume":"16","author":"Dower","year":"1988","journal-title":"Nucleic Acids Res."},{"key":"B17","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1128\/JB.161.1.292-298.1985","article-title":"Stable inheritance of plasmid R1 requires two different loci","volume":"161","author":"Gerdes","year":"1985","journal-title":"J. Bacteriol."},{"key":"B18","doi-asserted-by":"publisher","first-page":"7404","DOI":"10.1093\/emboj\/17.24.7404","article-title":"The structure of plasmid-encoded transcriptional repressor CopG unliganded and bound to its operator","volume":"17","author":"Gomis-R\u00fcth","year":"1998","journal-title":"EMBO J."},{"key":"B19","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1016\/j.plasmid.2012.01.005","article-title":"Fitness of the pMV158 replicon in Streptococcus pneumoniae","volume":"67","author":"Hern\u00e1ndez-Arriaga","year":"2012","journal-title":"Plasmid"},{"key":"B20","doi-asserted-by":"publisher","first-page":"4799","DOI":"10.1093\/nar\/gkp503","article-title":"Repressor CopG prevents access of RNA polymerase to promoter and actively dissociates open complexes","volume":"37","author":"Hern\u00e1ndez-Arriaga","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"B21","doi-asserted-by":"publisher","first-page":"210","DOI":"10.1016\/0147-619X(87)90029-1","article-title":"Plasmid repopulation kinetics in Staphylococcus aureus","volume":"17","author":"Highlander","year":"1987","journal-title":"Plasmid"},{"key":"B22","doi-asserted-by":"publisher","first-page":"5709","DOI":"10.1128\/JB.183.19.5709-5717.2001","article-title":"Genome of the bacterium Streptococcus pneumoniae strain R6","volume":"183","author":"Hoskins","year":"2001","journal-title":"J. Bacteriol."},{"key":"B23","doi-asserted-by":"publisher","first-page":"238","DOI":"10.1038\/291238a0","article-title":"Aromatic-dependent Salmonella typhimurium are non-virulent and effective as live vaccines","volume":"291","author":"Hoiseth","year":"1981","journal-title":"Nature"},{"key":"B24","doi-asserted-by":"publisher","first-page":"1289","DOI":"10.1093\/bioinformatics\/btm091","article-title":"Enhancements and modifications of primer design program Primer3","volume":"23","author":"Koressaar","year":"2007","journal-title":"Bioinformatics"},{"key":"B25","doi-asserted-by":"publisher","first-page":"709","DOI":"10.1038\/305709a0","article-title":"The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage","volume":"305","author":"Kreiswirth","year":"1983","journal-title":"Nature"},{"key":"B26","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/0022-2836(77)90289-3","article-title":"Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation","volume":"114","author":"Lacks","year":"1977","journal-title":"J. Mol. Biol."},{"key":"B27","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/0022-2836(86)90026-4","article-title":"Identification and analysis of genes for tetracycline resistance and replication functions in the broad-host-range plasmid pLS1","volume":"192","author":"Lacks","year":"1986","journal-title":"J. Mol. Biol."},{"key":"B28","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1128\/JB.157.2.445-453.1984","article-title":"Physical and genetic analyses of streptococcal plasmid pAM beta 1 and cloning of its replication region","volume":"157","author":"Leblanc","year":"1984","journal-title":"J. Bacteriol."},{"key":"B29","doi-asserted-by":"crossref","first-page":"692","DOI":"10.1128\/JB.150.2.692-701.1982","article-title":"Facilitation of plasmid transfer in Streptococcus pneumoniae by chromosomal homology","volume":"150","author":"L\u00f3pez","year":"1982","journal-title":"J. Bacteriol."},{"key":"B30","doi-asserted-by":"publisher","first-page":"225","DOI":"10.3389\/fgene.2015.00225","article-title":"The 5\u2032-tail of antisense RNAII of pMV158 plays a critical role in binding to the target mRNA and in translation inhibition of repB","volume":"6","author":"L\u00f3pez-Aguilar","year":"2015","journal-title":"Front. Genet."},{"key":"B31","doi-asserted-by":"publisher","first-page":"478","DOI":"10.3390\/toxins7020478","article-title":"Coupling between the basic replicon and the kis-kid maintenance system of plasmid R1: modulation by Kis antitoxin levels and involvement in control of plasmid replication","volume":"7","author":"L\u00f3pez-Villarejo","year":"2015","journal-title":"Toxins"},{"key":"B32","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1006\/jmbi.1993.1131","article-title":"Analysis of establishment phase replication of the plasmid ColE1","volume":"230","author":"Merlin","year":"1993","journal-title":"J. Mol. Biol."},{"key":"B33","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1128\/JB.138.1.70-79.1979","article-title":"Clustering of genes involved in replication, copy number control, incompatibility, and stable maintenance of the resistance plasmid R1drd-19","volume":"138","author":"Molin","year":"1979","journal-title":"J. Bacteriol."},{"key":"B34","doi-asserted-by":"publisher","first-page":"1121","DOI":"10.1016\/0092-8674(90)90405-4","article-title":"Control of plasmid replication - how do DNA iterons set the replication frequency?","volume":"63","author":"Nordstr\u00f6m","year":"1990","journal-title":"Cell"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.plasmid.2005.07.002","article-title":"Plasmid R1-Replication and its control","volume":"55","author":"Nordstr\u00f6m","year":"2006","journal-title":"Plasmid"},{"key":"B36","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/0147-619X(84)90054-4","article-title":"Control of replication of bacterial plasmids: genetics, molecular biology, and physiology of the plasmid R1 system","volume":"12","author":"Nordstr\u00f6m","year":"1984","journal-title":"Plasmid"},{"key":"B37","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1016\/0147-619X(85)90060-5","article-title":"Control of replication of FII plasmids: comparison of the basic replicons and of the copB systems of plasmids R100 and R1","volume":"13","author":"Nordstr\u00f6m","year":"1985","journal-title":"Plasmid"},{"key":"B38","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1128\/JB.186.1.207-211.2004","article-title":"Effect of the CopB auxiliary replication control system on stability of maintenance of Par+ plasmid R1","volume":"186","author":"Olsson","year":"2004","journal-title":"J. Bacteriol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"3459","DOI":"10.1038\/sj.emboj.7600815","article-title":"Kid cleaves specific mRNAs at UUACU sites to rescue the copy number of plasmid R1","volume":"24","author":"Pimentel","year":"2005","journal-title":"EMBO J."},{"key":"B40","doi-asserted-by":"publisher","first-page":"476","DOI":"10.1016\/j.mimet.2005.09.007","article-title":"The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative real-time PCR","volume":"65","author":"Providenti","year":"2006","journal-title":"J. Microbiol. Methods"},{"key":"B41","doi-asserted-by":"crossref","first-page":"5245","DOI":"10.1002\/j.1460-2075.1994.tb06856.x","article-title":"Replication-specific conversion of the Staphylococcus aureus pT181 initiator protein from an active homodimer to an inactive heterodimer","volume":"13","author":"Rasooly","year":"1994","journal-title":"EMBO J."},{"key":"B42","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1016\/0147-619X(86)90034-X","article-title":"Purification and characterization of the CopB replication control protein, and precise mapping of its target site in the R1 plasmid","volume":"15","author":"Riise","year":"1986","journal-title":"Plasmid"},{"key":"B43","doi-asserted-by":"publisher","first-page":"94","DOI":"10.1006\/plas.1993.1012","article-title":"Control of mini-R1 plasmid replication: a computer simulation","volume":"29","author":"Rosenfeld","year":"1993","journal-title":"Plasmid"},{"key":"B44","doi-asserted-by":"publisher","first-page":"113","DOI":"10.1016\/j.bbapap.2003.09.010","article-title":"Structural features of the initiator of replication protein RepB encoded by the promiscuous plasmid pMV158","volume":"1696","author":"Ruiz-Mas\u00f3","year":"2004","journal-title":"Biochim. Biophys. Acta Proteins Proteomics"},{"key":"B45","doi-asserted-by":"publisher","first-page":"1230","DOI":"10.1093\/nar\/gkl1099","article-title":"Interactions between the RepB initiator protein of plasmid pMV158 and two distant DNA regions within the origin of replication","volume":"35","author":"Ruiz-Mas\u00f3","year":"2007","journal-title":"Nucleic Acids Res."},{"key":"B46","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.PLAS-0035-2014","article-title":"Plasmid rolling-circle replication","author":"Ruiz-Mas\u00f3","year":"2015","journal-title":"Microbiol Spectr."},{"key":"B47","doi-asserted-by":"publisher","first-page":"7028","DOI":"10.1073\/pnas.78.11.7028","article-title":"Cloning of chromosomal genes in Streptococcus pneumoniae","volume":"78","author":"Stassi","year":"1981","journal-title":"Proc. Natl. Acad. Sci. U.S.A"},{"key":"B48","volume-title":"The Biology of Plasmids","author":"Summers","year":"2009"},{"key":"B49","doi-asserted-by":"publisher","first-page":"e115","DOI":"10.1093\/nar\/gks596","article-title":"Primer3\u2013new capabilities and interfaces","volume":"40","author":"Untergasser","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"B50","doi-asserted-by":"publisher","first-page":"361","DOI":"10.1016\/S0065-2660(02)46013-0","article-title":"Antisense RNAs in bacteria and their genetic elements","volume":"46","author":"Wagner","year":"2002","journal-title":"Adv. Genet."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fmicb.2017.02367\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,8,9]],"date-time":"2022-08-09T19:33:40Z","timestamp":1660073620000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fmicb.2017.02367\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,1]]},"references-count":50,"alternative-id":["10.3389\/fmicb.2017.02367"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2017.02367","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,1]]},"article-number":"2367"}}