{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T02:25:46Z","timestamp":1785551146045,"version":"3.56.0"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T00:00:00Z","timestamp":1525737600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009465","name":"Corporaci\u00f3n de Fomento de la Producci\u00f3n","doi-asserted-by":"publisher","award":["14IDL2-30005"],"award-info":[{"award-number":["14IDL2-30005"]}],"id":[{"id":"10.13039\/100009465","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2018.00923","type":"journal-article","created":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T07:14:16Z","timestamp":1525763656000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":26,"title":["Isolation, Functional Characterization and Transmissibility of p3PS10, a Multidrug Resistance Plasmid of the Fish Pathogen Piscirickettsia salmonis"],"prefix":"10.3389","volume":"9","author":[{"given":"Jos\u00e9","family":"Saavedra","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maritza","family":"Grand\u00f3n","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Villalobos-Gonz\u00e1lez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry","family":"Bohle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricio","family":"Bustos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos","family":"Mancilla","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,5,8]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"310","DOI":"10.1038\/nrmicro3439","article-title":"Tackling antibiotic resistance: the environmental framework","volume":"13","author":"Berendonk","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"B2","doi-asserted-by":"publisher","first-page":"e01571","DOI":"10.1128\/genomeA.01571-16","article-title":"The Genome sequence of an oxytetracycline-resistant isolate of the fish pathogen Piscirickettsia salmonis harbors a multidrug resistance plasmid","volume":"5","author":"Bohle","year":"2017","journal-title":"Genome Announc."},{"key":"B3","doi-asserted-by":"publisher","first-page":"e01219-14","DOI":"10.1128\/genomeA.01219-14","article-title":"Comparative genome analysis of two isolates of the fish pathogen Piscirickettsia salmonis from different hosts reveals major differences in virulence-associated secretion systems","volume":"2","author":"Bohle","year":"2014","journal-title":"Genome Announc."},{"key":"B4","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1016\/j.vetmic.2010.11.015","article-title":"The rarely reported tet(31) tetracycline resistance determinant is common in Gallibacterium anatis","volume":"149","author":"Bojesen","year":"2011","journal-title":"Vet. Microbiol."},{"key":"B5","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/j.vetmic.2016.10.015","article-title":"Whole-genome comparative analysis of the pathogen Piscirickettsia salmonis","volume":"196","author":"Bravo","year":"2016","journal-title":"Vet. Microbiol."},{"key":"B6","doi-asserted-by":"publisher","first-page":"e42724","DOI":"10.1371\/journal.pone.0042724","article-title":"Salmon aquaculture and antimicrobial resistance in the marine environment","volume":"7","author":"Buschmann","year":"2012","journal-title":"PLoS ONE"},{"key":"B7","doi-asserted-by":"publisher","first-page":"e127","DOI":"10.1016\/S1473-3099(16)00100-6","article-title":"Aquaculture as yet another environmental gateway to the development and globalisation of antimicrobial resistance","volume":"7","author":"Cabello","year":"2016","journal-title":"Lancet Infect. Dis"},{"key":"B8","doi-asserted-by":"publisher","first-page":"1025","DOI":"10.1111\/jfd.12579","article-title":"Search and analysis of genes involved in antibiotic resistance in Chilean strains of Piscirickettsia salmonis","volume":"40","author":"Cartes","year":"2016","journal-title":"J. Fish Dis"},{"key":"B9","volume-title":"Clinical and Laboratory Standards Institute. Methods for Broth Dilution Susceptibility Testing of Bacteria Isolated From Aquatic Animals","year":""},{"key":"B10","volume-title":"Clinical and Laboratory Standards Institute. Performance Standards for Antimicrobial Susceptibility Testing of Bacteria Isolated From Aquatic Animals, Second Informational Supplement","year":""},{"key":"B11","doi-asserted-by":"publisher","first-page":"1255","DOI":"10.3389\/fmicb.2017.01255","article-title":"A novel and validated protocol for performing MIC tests to determine the susceptibility of Piscirickettsia salmonis isolates to florfenicol and oxytetracycline","volume":"8","author":"Contreras-Lynch","year":"2017","journal-title":"Front. Microbiol."},{"key":"B12","doi-asserted-by":"publisher","first-page":"797","DOI":"10.1093\/jac\/dkr011","article-title":"Pc promoter from class 2 integrons and the cassette transcription pattern it evokes","volume":"66","author":"da Fonseca","year":"2011","journal-title":"J. Antimicrob. Chemother."},{"key":"B13","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.ijantimicag.2013.02.027","article-title":"Comparative analysis and distribution of pP9014, a novel drug resistance IncP-1 plasmid from Photobacterium damselae subsp. piscicida","volume":"42","author":"del Castillo","year":"2013","journal-title":"Int. J. Antimicrob. Agents"},{"key":"B14","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1016\/0044-8486(96)01253-7","article-title":"Bioavailability of oxytetracycline from medicated feed administered to Atlantic salmon (Salmo salar L.) in seawater","volume":"143","author":"Elema","year":"1996","journal-title":"Aquaculture"},{"key":"B15","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1099\/00207713-42-1-120","article-title":"Piscirickettsia salmonis gen. nov., sp. nov., the causative agent of an epizootic disease in salmonid fishes","volume":"42","author":"Fryer","year":"1992","journal-title":"Int. J. Syst. Bacteriol."},{"key":"B16","doi-asserted-by":"publisher","first-page":"83","DOI":"10.1111\/j.1574-6968.2011.02218.x","article-title":"Characterization of a functional toxin-antitoxin module in the genome of the fish pathogen Piscirickettsia salmonis","volume":"317","author":"G\u00f3mez","year":"2011","journal-title":"FEMS Microbiol. Lett."},{"key":"B17","doi-asserted-by":"publisher","first-page":"Unit1","DOI":"10.1002\/0471142727.mb0107s41","article-title":"Large-scale preparation of plasmid DNA","volume":"1","author":"Heilig","year":"2001","journal-title":"Curr. Protoc. Mol. Biol. Chapter"},{"key":"B18","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1111\/jfd.12427","article-title":"Comprehensive antibiotic susceptibility profiling of Chilean Piscirickettsia salmonis field isolates","volume":"39","author":"Henr\u00edquez","year":"2015","journal-title":"J. Fish Dis."},{"key":"B19","doi-asserted-by":"publisher","first-page":"e00810","DOI":"10.1128\/genomeA.00810-13","article-title":"Draft genome sequence of Gallibacterium anatis bv. haemolytica 12656-12 Liver, an isolate obtained from the liver of a septicemic chicken","volume":"1","author":"Kudirkiene","year":"2013","journal-title":"Genome Announc."},{"key":"B20","doi-asserted-by":"publisher","first-page":"5533","DOI":"10.1128\/AEM.66.12.5533-5535.2000","article-title":"Functional Tn5393-like transposon in the R plasmid pRAS2 from the fish pathogen Aeromonas salmonicida subspecies salmonicida isolated in Norway","volume":"66","author":"L'Abee-Lund","year":"2000","journal-title":"Appl. Environ. Microbiol."},{"key":"B21","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1080\/14760584.2017.1244483","article-title":"Vaccines for piscirickettsiosis (salmonid rickettsial septicaemia, SRS): the Chile perspective","volume":"16","author":"Maisey","year":"2016","journal-title":"Expert. Rev. Vaccines"},{"key":"B22","doi-asserted-by":"publisher","first-page":"3348","DOI":"10.1128\/AAC.00419-13","article-title":"The comprehensive antibiotic resistance database","volume":"57","author":"McArthur","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"B23","doi-asserted-by":"publisher","first-page":"883","DOI":"10.1128\/AAC.47.3.883-888.2003","article-title":"Diversity of tetracycline resistance genes in bacteria from Chilean salmon farms","volume":"47","author":"Miranda","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"B24","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1016\/j.aquaculture.2007.02.007","article-title":"Occurrence of florfenicol resistance in bacteria associated with two Chilean salmon farms with different history of antibacterial usage","volume":"266","author":"Miranda","year":"2007","journal-title":"Aquaculture"},{"key":"B25","doi-asserted-by":"publisher","first-page":"31","DOI":"10.1016\/S0044-8486(02)00124-2","article-title":"Bacterial resistance to oxytetracycline in Chilean salmon farming","volume":"212","author":"Miranda","year":"2002","journal-title":"Aquaculture"},{"key":"B26","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1186\/s12917-016-0681-0","article-title":"Phenotypic and genetic characterization of Piscirickettsia salmonis from Chilean and Canadian salmonids","volume":"12","author":"Otterlei","year":"2016","journal-title":"BMC Vet. Res"},{"key":"B27","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.jbiotec.2015.07.017","article-title":"Complete genome sequence of Piscirickettsia salmonis LF-89 (ATCC VR-1361) a major pathogen of farmed salmonid fish","volume":"212","author":"Pulgar","year":"2015","journal-title":"J. Biotechnol."},{"key":"B28","doi-asserted-by":"publisher","first-page":"699","DOI":"10.1128\/AAC.01392-08","article-title":"Novel insights about class 2 integrons from experimental and genomic epidemiology","volume":"54","author":"Ram\u00edrez","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"B29","doi-asserted-by":"publisher","first-page":"163","DOI":"10.1111\/jfd.12211","article-title":"Piscirickettsiosis and Piscirickettsia salmonis in fish: a review","volume":"37","author":"Rozas","year":"2014","journal-title":"J. Fish Dis."},{"key":"B30","doi-asserted-by":"publisher","first-page":"1055","DOI":"10.1111\/jfd.12581","article-title":"Prevalence, geographic distribution and phenotypic differences of Piscirickettsia salmonis EM-90-like isolates","volume":"40","author":"Saavedra","year":"2016","journal-title":"J. Fish Dis."},{"key":"B31","doi-asserted-by":"publisher","first-page":"519","DOI":"10.1016\/j.femsre.2004.04.001","article-title":"Molecular basis of bacterial resistance to chloramphenicol and florfenicol","volume":"28","author":"Schwarz","year":"2004","journal-title":"FEMS Microbiol. Rev"},{"key":"B32","volume-title":"Informe Sobre Uso de Antimicrobianos en la Salmonicultura Nacional 2014","year":"2015"},{"key":"B33","volume-title":"Informe Sobre Uso de Antimicrobianos en la Salmonicultura Nacional 2015","year":"2016"},{"key":"B34","volume-title":"Informe Sobre Uso de Antimicrobianos en la Salmonicultura Nacional 2016","year":"2017"},{"key":"B35","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.1111\/1462-2920.12421","article-title":"Antimicrobial resistance and antimicrobial resistance genes in marine bacteria from salmon aquaculture and non-aquaculture sites","volume":"16","author":"Shah","year":"2014","journal-title":"Environ. Microbiol."},{"key":"B36","doi-asserted-by":"publisher","first-page":"242","DOI":"10.3389\/fmicb.2015.00242","article-title":"Genomics of microbial plasmids: classification and identification based on replication and transfer systems and host taxonomy","volume":"6","author":"Shintani","year":"2015","journal-title":"Front. Microbiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1788","DOI":"10.1128\/JB.184.6.1788-1793.2002","article-title":"The active partition gene incC of IncP plasmids is required for stable maintenance in a broad range of hosts","volume":"184","author":"Siddique","year":"2002","journal-title":"J. Bacteriol."},{"key":"B38","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1054\/drup.2000.0146","article-title":"Sulfonamide resistance: mechanisms and trends","volume":"3","author":"Sk\u00f6ld","year":"2000","journal-title":"Drug Resist. Updat."},{"key":"B39","doi-asserted-by":"publisher","first-page":"6","DOI":"10.1186\/1475-2859-7-6","article-title":"Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes","volume":"7","author":"Skulj","year":"2008","journal-title":"Microb. Cell Fact."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.1128\/AAC.47.4.1285-1290.2003","article-title":"Integron-containing IncU R plasmids pRAS1 and pAr-32 from the fish pathogen Aeromonas salmonicida","volume":"47","author":"S\u00f8rum","year":"2003","journal-title":"Antimicrob. Agents Chemother."},{"key":"B41","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1007\/s00018-009-0172-6","article-title":"The tetracycline resistome","volume":"67","author":"Thaker","year":"2009","journal-title":"Cell. Mol. Life Sci."},{"key":"B42","doi-asserted-by":"publisher","first-page":"e26219","DOI":"10.4161\/mge.26219","article-title":"Toxin-antitoxin systems: biology, identification, and application","volume":"3","author":"Unterholzner","year":"2013","journal-title":"Mob. Genet. Elements"},{"key":"B43","doi-asserted-by":"publisher","first-page":"e7646","DOI":"10.1371\/journal.pone.0007646","article-title":"Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches","volume":"4","author":"Wang","year":"2009","journal-title":"PLoS ONE"},{"key":"B44","doi-asserted-by":"publisher","first-page":"197","DOI":"10.3354\/dao02403","article-title":"Broth medium for the successful culture of the fish pathogen Piscirickettsia salmonis","volume":"97","author":"Ya\u00f1ez","year":"2012","journal-title":"Dis. Aquat. Org."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fmicb.2018.00923\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T07:14:23Z","timestamp":1525763663000},"score":1,"resource":{"primary":{"URL":"http:\/\/journal.frontiersin.org\/article\/10.3389\/fmicb.2018.00923\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,8]]},"references-count":44,"alternative-id":["10.3389\/fmicb.2018.00923"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2018.00923","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,8]]},"article-number":"923"}}