{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:55:33Z","timestamp":1790772933309,"version":"4.1.0"},"reference-count":47,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T00:00:00Z","timestamp":1530230400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2018.01422","type":"journal-article","created":{"date-parts":[[2018,6,29]],"date-time":"2018-06-29T13:30:44Z","timestamp":1530279044000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":68,"title":["Interplay Between Membrane Permeability and Enzymatic Barrier Leads to Antibiotic-Dependent Resistance in Klebsiella Pneumoniae"],"prefix":"10.3389","volume":"9","author":[{"given":"Marie-Helene","family":"Nicolas-Chanoine","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00e9mie","family":"Mayer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathleen","family":"Guyot","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Dumont","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Marie","family":"Pag\u00e8s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,6,29]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1016\/j.ijantimicag.2011.09.012","article-title":"In vitro activity of avibactam (NXL104) in combination with \u03b2-lactams against Gram-negative bacteria, including OXA-48 \u03b2-lactamase-producing Klebsiella pneumoniae","volume":"39","author":"Akta\u015f","year":"2012","journal-title":"Int. J. Antimicrob. Agents"},{"key":"B2","doi-asserted-by":"publisher","first-page":"1992","DOI":"10.1128\/AAC.02571-12","article-title":"Comparative population analysis of Klebsiella pneumoniae strains with extended-spectrum \u03b2-lactamases colonizing patients in rehabilitation centers in four countries","volume":"57","author":"Baraniak","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"B3","doi-asserted-by":"publisher","first-page":"4373","DOI":"10.1128\/AAC.01607-09","article-title":"Membrane efflux and influx modulate both multidrug resistance and virulence of Klebsiella pneumoniae in a Caenorhabditis elegans model","volume":"54","author":"Bialek","year":"2010","journal-title":"Antimicrob. Agents Chemother."},{"key":"B4","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1093\/jac\/dku340","article-title":"Differential contribution of AcrAB and OqxAB efflux pumps to multidrug resistance and virulence in Klebsiella pneumoniae","volume":"70","author":"Bialek-Davenet","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"B5","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1128\/AAC.01410-12","article-title":"Complete deletion of the ramR gene in an in vitro-selected mutant of Klebsiella pneumoniae overexpressing the AcrAB efflux pump","volume":"57","author":"Bialek-Davenet","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"B6","doi-asserted-by":"publisher","first-page":"2795","DOI":"10.1128\/AAC.00156-11","article-title":"In vitro selection of ramR and soxR mutants overexpressing efflux systems by fluoroquinolones as well as cefoxitin in Klebsiella pneumoniae","volume":"55","author":"Bialek-Davenet","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"B7","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/j.diagmicrobio.2007.09.017","article-title":"Use of tigecycline for the treatment of prolonged bacteremia due to a multiresistant VIM-1 and SHV-12 \u03b2-lactamase-producing Klebsiella pneumoniae epidemic clone","volume":"60","author":"Cobo","year":"2008","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"B8","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.ijantimicag.2009.10.021","article-title":"In vivo selection of imipenem-resistant Klebsiella pneumoniae producing extended-spectrum \u03b2-lactamase CTX-M-15 and plasmid-encoded DHA-1 cephalosporinase","volume":"35","author":"Cuzon","year":"2010","journal-title":"Int. J. Antimicrob. Agents"},{"key":"B9","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1089\/mdr.2011.0214","article-title":"Imipenem resistance in Klebsiella pneumoniae is associated to the combination of plasmid-mediated CMY-4 AmpC \u03b2-lactamase and loss of an outer membrane protein","volume":"18","author":"Dahmen","year":"2012","journal-title":"Microb. Drug Resist."},{"key":"B10","doi-asserted-by":"publisher","first-page":"1603","DOI":"10.1128\/AAC.01998-12","article-title":"Elucidating the regulon of multidrug resistance regulator RarA in Klebsiella pneumoniae","volume":"57","author":"De Majumdar","year":"2013","journal-title":"Antimicrob. Agents Chemother."},{"key":"B11","doi-asserted-by":"publisher","first-page":"e1004627","DOI":"10.1371\/journal.ppat.1004627","article-title":"Elucidation of the RamA regulon in Klebsiella pneumoniae reveals a role in LPS regulation","volume":"11","author":"De Majumdar","year":"2015","journal-title":"PLOS Pathog."},{"key":"B12","doi-asserted-by":"publisher","first-page":"e95","DOI":"10.1086\/503264","article-title":"In vivo development of ertapenem resistance in a patient with pneumonia caused by Klebsiella pneumoniae with an extended-spectrum \u03b2-lactamase","volume":"42","author":"Elliott","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"B13","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1046\/j.1469-0691.2003.00790","article-title":"Determination of minimum inhibitory concentrations (MICs) of antibacterial agents by broth dilution","volume":"9","year":"2003","journal-title":"Clin. Microbiol. Infect."},{"key":"B14","doi-asserted-by":"publisher","first-page":"e0165019","DOI":"10.1371\/journal.pone.0165019","article-title":"Step-wise increase in tigecycline resistance in Klebsiella pneumoniae associated with mutations in ramR, lon and rpsJ","volume":"11","author":"Fang","year":"2016","journal-title":"PLoS ONE"},{"key":"B15","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1007\/s10096-013-1983-9","article-title":"Long-term dissemination of acquired AmpC \u03b2-lactamases among Klebsiella spp. and Escherichia coli in Portuguese clinical settings","volume":"33","author":"Freitas","year":"2014","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"B16","doi-asserted-by":"publisher","first-page":"1879","DOI":"10.2165\/11315690-000000000-00000","article-title":"Multidrug-resistant Gram-negative infections: what are the treatment options?","volume":"69","author":"Giamarellou","year":"2009","journal-title":"Drugs"},{"key":"B17","doi-asserted-by":"publisher","first-page":"2701","DOI":"10.1128\/JCM.42.6.2701-2706.2004","article-title":"Detection and prevalence of active drug efflux mechanism in various multidrug-resistant Klebsiella pneumoniae strains from Turkey","volume":"42","author":"Hasdemir","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"B18","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1128\/AAC.43.4.937","article-title":"Development of resistance during antimicrobial therapy caused by insertion sequence interruption of porin genes","volume":"43","author":"Hern\u00e1ndez-All\u00e9s","year":"1999","journal-title":"Antimicrob. Agents Chemother."},{"key":"B19","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1093\/clinids\/10.4.867","article-title":"Extended broad-spectrum \u03b2-lactamases conferring transferable resistance to newer \u03b2-lactam agents in Enterobacteriaceae: hospital prevalence and susceptibility patterns","volume":"10","author":"Jarlier","year":"1988","journal-title":"Rev. Infect. Dis."},{"key":"B20","doi-asserted-by":"publisher","first-page":"2278","DOI":"10.1093\/jac\/dkx136","article-title":"Activity of ceftolozane\/tazobactam against surveillance and \u201cproblem\u201d Enterobacteriaceae, Pseudomonas aeruginosa and non-fermenters from the British Isles","volume":"72","author":"Livermore","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"B21","doi-asserted-by":"publisher","first-page":"632","DOI":"10.1093\/jac\/dkt419","article-title":"Genomic analysis of the emergence and evolution of multidrug resistance during a Klebsiella pneumoniae outbreak including carbapenem and colistin resistance","volume":"69","author":"L\u00f3pez-Camacho","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"B22","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1016\/j.diagmicrobio.2016.03.009","article-title":"High-level carbapenem-resistant OXA-48-producing Klebsiella pneumoniae with a novel OmpK36 variant and low-level, carbapenem-resistant, non-porin-deficient, OXA-181-producing Escherichia coli from Thailand","volume":"85","author":"Lunha","year":"2016","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"B23","doi-asserted-by":"publisher","first-page":"1387","DOI":"10.1128\/AAC.44.5.1387-1390.2000","article-title":"Contributions of the AmpC \u03b2-lactamase and the AcrAB multidrug efflux system in intrinsic resistance of Escherichia coli K-12 to \u03b2-lactams","volume":"44","author":"Mazzariol","year":"2000","journal-title":"Antimicrob. Agents Chemother."},{"key":"B24","doi-asserted-by":"publisher","first-page":"1791","DOI":"10.3201\/eid1710.110655","article-title":"Global spread of carbapenemase-producing Enterobacteriaceae","volume":"17","author":"Nordmann","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"B25","doi-asserted-by":"publisher","first-page":"722","DOI":"10.1128\/AAC.01866-13","article-title":"Characterization of a novel pyranopyridine inhibitor of the AcrAB efflux pump of Escherichia coli","volume":"58","author":"Opperman","year":"2014","journal-title":"Antimicrob. Agents Chemother."},{"key":"B26","doi-asserted-by":"publisher","first-page":"e4817","DOI":"10.1371\/journal.pone.0004817","article-title":"Efflux pump, the masked side of \u03b2-lactam resistance in Klebsiella pneumoniae clinical isolates","volume":"4","author":"Pag\u00e8s","year":"2009","journal-title":"PLoS ONE"},{"key":"B27","doi-asserted-by":"publisher","first-page":"1005","DOI":"10.1093\/infdis\/159.5.1005","article-title":"In vivo selection of a cephamycin-resistant, porin-deficient mutant of Klebsiella pneumoniae producing a TEM-3 \u03b2-lactamase","volume":"159","author":"Pangon","year":"1989","journal-title":"J. Infect. Dis."},{"key":"B28","doi-asserted-by":"publisher","first-page":"353","DOI":"10.3343\/alm.2013.33.5.353","article-title":"An increase in the clinical isolation of acquired AmpC \u03b2-lactamase-producing Klebsiella pneumoniae in Korea from 2007 to 2010","volume":"33","author":"Park","year":"2013","journal-title":"Ann. Lab. Med."},{"key":"B29","doi-asserted-by":"publisher","first-page":"297","DOI":"10.1586\/eri.13.12","article-title":"Clinical relevance of the ESKAPE pathogens","volume":"11","author":"Pendleton","year":"2013","journal-title":"Expert Rev. Anti Infect. Ther."},{"key":"B30","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1517\/14656566.2016.1145658","article-title":"Treatment options for infections caused by carbapenem-resistant Enterobacteriaceae: can we apply \u201cprecision medicine\u201d to antimicrobial chemotherapy?","volume":"17","author":"Perez","year":"2016","journal-title":"Expert Opin. Pharmacother."},{"key":"B31","doi-asserted-by":"publisher","first-page":"e0138828","DOI":"10.1371\/journal.pone.0138828","article-title":"In vivo evolution of bacterial resistance in two cases of Enterobacter aerogenes infections during treatment with imipenem","volume":"10","author":"Philippe","year":"2015","journal-title":"PLoS ONE"},{"key":"B32","doi-asserted-by":"crossref","first-page":"589","DOI":"10.1128\/CMR.11.4.589","article-title":"Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors","volume":"11","author":"Podschun","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"B33","doi-asserted-by":"publisher","first-page":"20549","DOI":"10.2807\/1560-7917.ES2013.18.31.20549","article-title":"Intercontinental spread of OXA-48 \u03b2-lactamase-producing Enterobacteriaceae over a 11-year period, 2001 to 2011","volume":"18","author":"Potron","year":"2013","journal-title":"Euro Surveill."},{"key":"B34","first-page":"11","article-title":"\u03b2-lactamase insensitive or inhibitory beta-lactams: two approaches to the challenge of ampicillin-resistant E. coli","volume":"13","author":"Richmond","year":"1978","journal-title":"Scand. J. Infect. Dis. Suppl."},{"key":"B35","doi-asserted-by":"publisher","first-page":"2706","DOI":"10.1093\/jac\/dku208","article-title":"Incidence rates of carbapenemase-producing Enterobacteriaceae clinical isolates in France: a prospective nationwide study in 2011-12","volume":"69","author":"Robert","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"B36","doi-asserted-by":"publisher","first-page":"1100","DOI":"10.1016\/j.ijmm.2014.08.003","article-title":"Expansion of ESBL-producing Klebsiella pneumoniae in hospitalized patients: a successful story of international clones (ST15, ST147, ST336) and epidemic plasmids (IncR, IncFIIK)","volume":"304","author":"Rodrigues","year":"2014","journal-title":"Int. J. Med. Microbiol."},{"key":"B37","doi-asserted-by":"publisher","first-page":"348","DOI":"10.1093\/jac\/dkv355","article-title":"A single-day point-prevalence study of faecal carriers in long-term care hospitals in Madrid (Spain) depicts a complex clonal and polyclonal dissemination of carbapenemase-producing Enterobacteriaceae","volume":"71","author":"Ruiz-Garbajosa","year":"2016","journal-title":"J. Antimicrob. Chemother."},{"key":"B38","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1590\/S1517-83822013000200015","article-title":"Carbapenem and cefoxitin resistance of Klebsiella pneumoniae strains associated with porin OmpK36 loss and DHA-1 \u03b2-lactamase production","volume":"44","author":"Shi","year":"2013","journal-title":"Braz. J. Microbiol."},{"key":"B39","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1099\/jmm.0.012468-0","article-title":"Development of ertapenem resistance in a patient with mediastinitis caused by Klebsiella pneumoniae producing an extended-spectrum beta-lactamase","volume":"59","author":"Skurnik","year":"2010","journal-title":"J. Med. Microbiol."},{"key":"B40","doi-asserted-by":"publisher","first-page":"1126","DOI":"10.1128\/AAC.45.4.1126-1136.2001","article-title":"Antibiotic susceptibility profiles of Escherichia coli strains lacking multidrug efflux pump genes","volume":"45","author":"Sulavik","year":"2001","journal-title":"Antimicrob. Agents Chemother."},{"key":"B41","doi-asserted-by":"publisher","first-page":"972","DOI":"10.1093\/cid\/cix034","article-title":"The use of non-carbapenem \u03b2-Lactams for the treatment of extended-spectrum \u03b2-Lactamase infections","volume":"64","author":"Tamma","year":"2017","journal-title":"Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am."},{"key":"B42","doi-asserted-by":"publisher","first-page":"137","DOI":"10.1016\/j.diagmicrobio.2011.02.004","article-title":"In vitro activity of CXA-101 plus tazobactam (CXA-201) against CTX-M-14- and CTX-M-15-producing Escherichia coli and Klebsiella pneumoniae","volume":"70","author":"Titelman","year":"2011","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"B43","doi-asserted-by":"publisher","first-page":"7842","DOI":"10.1128\/AAC.02019-15","article-title":"In vitro activities of ceftazidime-avibactam, aztreonam -avibactam, and a panel of older and contemporary antimicrobial agents against carbapenemase-producing Gram-negative bacilli","volume":"59","author":"Vasoo","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"B44","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1093\/jac\/dks530","article-title":"Genetic characterization of tigecycline resistance in clinical isolates of Enterobacter cloacae and Enterobacter aerogenes","volume":"68","author":"Veleba","year":"2013","journal-title":"J. Antimicrob. Chemother."},{"key":"B45","doi-asserted-by":"publisher","first-page":"e365","DOI":"10.1371\/journal.pone.0000365","article-title":"Antibiotic stress, genetic response and altered permeability of E. coli","volume":"2","author":"Viveiros","year":"2007","journal-title":"PLoS ONE"},{"key":"B46","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1093\/jac\/dkt383","article-title":"In vitro activity of temocillin against multidrug-resistant clinical isolates of Escherichia coli, Klebsiella spp. and Enterobacter spp., and evaluation of high-level temocillin resistance as a diagnostic marker for OXA-48 carbapenemase","volume":"69","author":"Woodford","year":"2014","journal-title":"J. Antimicrob. Chemother."},{"key":"B47","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1016\/j.tim.2016.09.007","article-title":"Klebsiella pneumoniae population genomics and antimicrobial-resistant clones","volume":"24","author":"Wyres","year":"2016","journal-title":"Trends Microbiol."}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2018.01422\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,10,20]],"date-time":"2019-10-20T05:06:44Z","timestamp":1571548004000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2018.01422\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,29]]},"references-count":47,"alternative-id":["10.3389\/fmicb.2018.01422"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2018.01422","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,6,29]]},"article-number":"1422"}}