{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T18:06:09Z","timestamp":1787508369136,"version":"build-2736575974"},"reference-count":44,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T00:00:00Z","timestamp":1534896000000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2014\/13412-8"],"award-info":[{"award-number":["2014\/13412-8"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["476661\/2013-7"],"award-info":[{"award-number":["476661\/2013-7"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["308955\/2012-9"],"award-info":[{"award-number":["308955\/2012-9"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["frontiersin.org"],"crossmark-restriction":true},"short-container-title":["Front. Microbiol."],"DOI":"10.3389\/fmicb.2018.01780","type":"journal-article","created":{"date-parts":[[2018,8,22]],"date-time":"2018-08-22T00:18:06Z","timestamp":1534897086000},"update-policy":"https:\/\/doi.org\/10.3389\/crossmark-policy","source":"Crossref","is-referenced-by-count":6,"title":["HU-Lacking Mutants of Salmonella enterica Enteritidis Are Highly Attenuated and Can Induce Protection in Murine Model of Infection"],"prefix":"10.3389","volume":"9","author":[{"given":"Guilherme P.","family":"Milanez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catierine H.","family":"Werle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariene R.","family":"Amorim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael A.","family":"Ribeiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz H. S.","family":"Tibo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Cristina","family":"Roque-Barreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aline F.","family":"Oliveira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcelo","family":"Brocchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2018,8,22]]},"reference":[{"key":"B1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004500","article-title":"Silencing by H-NS potentiated the evolution of Salmonella.","volume":"10","author":"Ali","year":"2014","journal-title":"PLoS Pathog."},{"key":"B2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0016667","article-title":"Salmonella enterica serovar Typhimurium lacking hfq gene confers protective immunity against murine typhoid.","volume":"6","author":"Allam","year":"2011","journal-title":"PLoS One"},{"key":"B3","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00491","article-title":"Macrophage cytokines: involvement in immunity and infectious diseases.","volume":"5","author":"Arango Duque","year":"2014","journal-title":"Front. Immunol."},{"key":"B4","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1038\/embor.2009.232","article-title":"Coordination of genomic structure and transcription by the main bacterial nucleoid-associated protein HU.","volume":"11","author":"Berger","year":"2010","journal-title":"EMBO Rep."},{"key":"B5","doi-asserted-by":"publisher","DOI":"10.1038\/srep31512","article-title":"Genes on a wire: the nucleoid- associated protein HU insulates transcription units in Escherichia coli.","volume":"6","author":"Berger","year":"2016","journal-title":"Sci. Rep."},{"key":"B6","doi-asserted-by":"publisher","first-page":"1403","DOI":"10.1017\/S0950268814000491","article-title":"Characterization of Salmonella Enteritidis strains isolated from poultry and farm environments in Brazil.","volume":"142","author":"Campioni","year":"2014","journal-title":"Epidemiol. Infect."},{"key":"B7","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1006\/jmbi.1997.1310","article-title":"Variation in HU composition during growth of Escherichia coli: the heterodimer is required for long term survival.","volume":"273","author":"Claret","year":"1997","journal-title":"J. Mol. Biol."},{"key":"B8","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1111\/j.1365-2958.1996.tb02664.x","article-title":"Virulence and vaccine potential of Salmonella typhimurium mutants deficient in the expression of the RpoS (\u03c3(S)) regulon.","volume":"22","author":"Coynault","year":"1996","journal-title":"Mol. Microbiol."},{"key":"B9","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1128\/CMR.00002-15","article-title":"Epidemiology, clinical presentation, laboratory diagnosis, antimicrobial resistance, and antimicrobial management of invasive Salmonella infections.","volume":"28","author":"Crump","year":"2015","journal-title":"Clin. Microbiol. Rev."},{"key":"B10","doi-asserted-by":"crossref","first-page":"3035","DOI":"10.1128\/IAI.55.12.3035-3043.1987","article-title":"Salmonella typhimurium deletion mutants lacking adenylate cyclase and cyclic AMP receptor protein are avirulent and immunogenic.","volume":"55","author":"Curtiss","year":"1987","journal-title":"Infect. Immun."},{"key":"B11","doi-asserted-by":"publisher","first-page":"4523","DOI":"10.1016\/j.vaccine.2017.07.035","article-title":"A recombinant protein of Salmonella Typhi induces humoral and cell-mediated immune responses including memory responses.","volume":"35","author":"Das","year":"2017","journal-title":"Vaccine"},{"key":"B12","doi-asserted-by":"publisher","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B13","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002933","article-title":"Host-Pathogen interaction in invasive Salmonellosis.","volume":"8","author":"de Jong","year":"2012","journal-title":"PLoS Pathog."},{"key":"B14","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1586\/erv.12.138","article-title":"Salmonella vaccines in poultry: past, present and future.","volume":"12","author":"Desin","year":"2013","journal-title":"Expert Rev. Vaccines"},{"key":"B15","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1038\/nrmicro2261","article-title":"Bacterial nucleoid-associated proteins, nucleoid structure and gene expression.","volume":"8","author":"Dillon","year":"2010","journal-title":"Nat. Rev. Microbiol."},{"key":"B16","doi-asserted-by":"crossref","first-page":"732","DOI":"10.1128\/IAI.66.2.732-740.1998","article-title":"Comparison of the abilities of different attenuated Salmonella typhimurium strains to elicit humoral immune responses against a heterologous antigen.","volume":"66","author":"Dunstan","year":"1998","journal-title":"Infect. Immun."},{"key":"B17","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1016\/0076-6879(94)36030-8","article-title":"Measurement of invasion by gentamicin resistance.","volume":"236","author":"Elsinghorst","year":"1994","journal-title":"Methods Enzymol."},{"key":"B18","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2016.00148","article-title":"Protective immunity elicited by oral immunization of mice with Salmonella enterica Serovar Typhimurium Braun Lipoprotein (Lpp) and Acetyltransferase (MsbB) Mutants.","volume":"6","author":"Erova","year":"2016","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"B19","doi-asserted-by":"publisher","first-page":"2489","DOI":"10.1016\/S0140-6736(11)61752-2","article-title":"Invasive non-typhoidal salmonella disease: an emerging and neglected tropical disease in Africa.","volume":"379","author":"Feasey","year":"2012","journal-title":"Lancet"},{"key":"B20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003979","article-title":"Modelling the contributions of Malaria, HIV, malnutrition and rainfall to the decline in paediatric invasive non-typhoidal Salmonella disease in malawi.","volume":"9","author":"Feasey","year":"2015","journal-title":"PLoS Negl. Trop. Dis."},{"key":"B21","doi-asserted-by":"publisher","first-page":"4273","DOI":"10.1128\/AEM.00598-11","article-title":"Population dynamics of Salmonella enterica serotypes in commercial egg and poultry production.","volume":"77","author":"Foley","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"B22","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/0882-4010(89)90085-5","article-title":"Virulence and vaccine potential of phoP mutants of Salmonella typhimurium.","volume":"6","author":"Gal\u00e1n","year":"1989","journal-title":"Microb. Pathog."},{"key":"B23","doi-asserted-by":"publisher","first-page":"4376","DOI":"10.1016\/j.vaccine.2013.12.026","article-title":"The delicate balance in genetically engineering live vaccines.","volume":"32","author":"Galen","year":"2014","journal-title":"Vaccine"},{"key":"B24","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/j.cimid.2011.07.001","article-title":"Evaluation of Salmonella enterica serovar Typhimurium and Choleraesuis slyA mutant strains for use in live attenuated oral vaccines.","volume":"34","author":"Haneda","year":"2011","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"B25","doi-asserted-by":"publisher","first-page":"3457","DOI":"10.1128\/IAI.70.7.3457-3467.2002","article-title":"Characterization of Salmonella enterica derivatives harboring defined aroC and Salmonella pathogenicity Island 2 Type III secretion system (ssaV) mutations by immunization of healthy volunteers.","volume":"70","author":"Hindle","year":"2002","journal-title":"Infect. Immun."},{"key":"B26","doi-asserted-by":"publisher","first-page":"251","DOI":"10.1016\/0264-410X(95)00249-Z","article-title":"Protection against oral challenge three months after i.v. immunization of BALBc mice with live Aro Salmonella typhimurium and Salmonella enteritidis vaccines is serotype (species)-dependent and only partially determined by the main LPS O antigen.","volume":"14","author":"Hormaeche","year":"1996","journal-title":"Vaccine"},{"key":"B27","doi-asserted-by":"publisher","first-page":"16397","DOI":"10.1073\/pnas.0508032102","article-title":"Nucleoid remodeling by an altered HU protein: reorganization of the transcription program.","volume":"102","author":"Kar","year":"2005","journal-title":"Proc. Natl. Acad. Sci. U.S.A."},{"key":"B28","doi-asserted-by":"publisher","first-page":"206","DOI":"10.1038\/nrmicro3428","article-title":"Now you see me, now you don\u2019t: the interaction of Salmonella with innate immune receptors.","volume":"13","author":"Keestra-Gounder","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"B29","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1016\/S0140-6736(13)60844-2","article-title":"Burden and aetiology of diarrhoeal disease in infants and young children in developing countries (the Global Enteric Multicenter Study, GEMS): a prospective, case-control study.","volume":"382","author":"Kotloff","year":"2013","journal-title":"Lancet"},{"key":"B30","doi-asserted-by":"publisher","first-page":"4997","DOI":"10.1128\/IAI.02192-r14","article-title":"Cellular requirements for systemic control of Salmonella enterica serovar Typhimurium infections in mice.","volume":"82","author":"Kupz","year":"2014","journal-title":"Infect. Immun."},{"key":"B31","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1586\/eri.13.27","article-title":"Invasive nontyphoidal Salmonella disease in Africa: current status.","volume":"11","author":"MacLennan","year":"2013","journal-title":"Expert Rev. Anti. Infect. Ther."},{"key":"B32","doi-asserted-by":"publisher","first-page":"1478","DOI":"10.4161\/hv.29054","article-title":"Vaccines against invasive Salmonella disease current status and future directions.","volume":"10","author":"Maclennan","year":"2014","journal-title":"Hum. Vaccines Immunother."},{"key":"B33","doi-asserted-by":"publisher","first-page":"1075","DOI":"10.1099\/mic.0.046359-0","article-title":"Nucleoid-associated protein HU controls three regulons that coordinate virulence, response to stress and general physiology in Salmonella enterica serovar typhimurium.","volume":"157","author":"Mangan","year":"2011","journal-title":"Microbiology"},{"key":"B34","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1053\/tvjl.2000.0502","article-title":"Salmonella: immune responses and vaccines.","volume":"161","author":"Mastroeni","year":"2001","journal-title":"Vet. J."},{"key":"B35","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00340-16","article-title":"Whole-genome sequence of Salmonella enterica serovar Enteritidis phage Type 4, Isolated from a Brazilian poultry farm.","volume":"4","author":"Milanez","year":"2016","journal-title":"Genome Annoucements"},{"key":"B36","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1016\/j.micinf.2006.12.019","article-title":"Oral administration of a live attenuated Salmonella vaccine strain expressing the VapA protein induces protection against infection by Rhodococcus equi.","volume":"9","author":"Oliveira","year":"2007","journal-title":"Microbes Infect."},{"key":"B37","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1016\/S1286-4579(01)01486-1","article-title":"Bacterial virulence, proinflammatory cytokines and host immunity: how to choose the appropriate Salmonella vaccine strain?","volume":"3","author":"Raupach","year":"2001","journal-title":"Microbes Infect."},{"key":"B38","doi-asserted-by":"publisher","first-page":"26177","DOI":"10.1074\/jbc.M502838200","article-title":"A superhelical spiral in the Escherichia coli DNA gyrase A C-terminal domain imparts unidirectional supercoiling bias.","volume":"280","author":"Ruthenburg","year":"2005","journal-title":"J. Biol. Chem."},{"key":"B39","author":"Sambrook","year":"2003","journal-title":"Molecular Cloning - a Laboratory Manual"},{"key":"B40","doi-asserted-by":"publisher","first-page":"5432","DOI":"10.1128\/IAI.71.9.5432-5435.2003","article-title":"The small nucleoid-binding proteins H-NS, HU, and Fis affect hilA expression in Salmonella enterica serovar typhimurium.","volume":"71","author":"Schechter","year":"2003","journal-title":"Infect. Immun."},{"key":"B41","doi-asserted-by":"publisher","first-page":"3987","DOI":"10.1128\/IAI.72.7.3987-4003.2004","article-title":"The two murein lipoproteins of Salmonella enterica serovar typhimurium contribute to the virulence of the organism.","volume":"72","author":"Sha","year":"2004","journal-title":"Infect. Immun."},{"key":"B42","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1080\/00365540410026068","article-title":"Serum levels of interferon-\u03b3, interleukin-12, tumour necrosis factor-\u03b1, and interleukin-10, and bacterial clearance in patients with gastroenteric Salmonella infection.","volume":"37","author":"Stoycheva","year":"2005","journal-title":"Scand. J. Infect. Dis."},{"key":"B43","doi-asserted-by":"publisher","first-page":"C36","DOI":"10.1016\/j.vaccine.2015.04.029","article-title":"Live attenuated vaccines for invasive Salmonella infections.","volume":"33","author":"Tennant","year":"2015","journal-title":"Vaccine"},{"key":"B44","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1128\/IAI.66.5.2099-2106.1998","article-title":"Identification of Salmonella typhimurium genes required for colonization of the chicken alimentary tract and for virulence in newly hatched chicks.","volume":"66","author":"Turner","year":"1998","journal-title":"Infect. Immun"}],"container-title":["Frontiers in Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2018.01780\/full","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,11,7]],"date-time":"2020-11-07T21:40:49Z","timestamp":1604785249000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontiersin.org\/article\/10.3389\/fmicb.2018.01780\/full"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,22]]},"references-count":44,"alternative-id":["10.3389\/fmicb.2018.01780"],"URL":"https:\/\/doi.org\/10.3389\/fmicb.2018.01780","relation":{},"ISSN":["1664-302X"],"issn-type":[{"value":"1664-302X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,22]]},"article-number":"1780"}}